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- W4328104235 abstract "A better understanding of wheat functional genomics can improve targeted breeding for better agronomic traits and environmental adaptation. However, the lack of gene-indexed mutants and the low transformation efficiency of wheat limit in-depth gene functional studies and genetic manipulation for breeding. In this study, we created a library for KN9204, a popular wheat variety in northern China, with a reference genome, transcriptome, and epigenome of different tissues, using ethyl methyl sulfonate (EMS) mutagenesis. This library contains a vast developmental diversity of critical tissues and transition stages. Exome capture sequencing of 2090 mutant lines using KN9204 genome-designed probes revealed that 98.79% of coding genes had mutations, and each line had an average of 1383 EMS-type SNPs. We identified new allelic variations for crucial agronomic trait-related genes such as Rht-D1, Q, TaTB1, and WFZP. We tested 100 lines with severe mutations in 80 NAC transcription factors (TFs) under drought and salinity stress and identified 13 lines with altered sensitivity. Further analysis of three lines using transcriptome and chromatin accessibility data revealed hundreds of direct NAC targets with altered transcription patterns under salt or drought stress, including SNAC1, DREB2B, CML16, and ZFP182, factors known to respond to abiotic stress. Thus, we have generated and indexed a KN9204 EMS mutant library that can facilitate functional genomics research and offer resources for genetic manipulation of wheat." @default.
- W4328104235 created "2023-03-22" @default.
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- W4328104235 date "2023-07-01" @default.
- W4328104235 modified "2023-10-16" @default.
- W4328104235 title "Boosting wheat functional genomics via an indexed EMS mutant library of KN9204" @default.
- W4328104235 cites W1734091752 @default.
- W4328104235 cites W1942036305 @default.
- W4328104235 cites W2013266273 @default.
- W4328104235 cites W2015587562 @default.
- W4328104235 cites W2032389129 @default.
- W4328104235 cites W2035618305 @default.
- W4328104235 cites W2037558363 @default.
- W4328104235 cites W2093067391 @default.
- W4328104235 cites W2106578986 @default.
- W4328104235 cites W2115450398 @default.
- W4328104235 cites W2118527625 @default.
- W4328104235 cites W2120249321 @default.
- W4328104235 cites W2125105412 @default.
- W4328104235 cites W2128215659 @default.
- W4328104235 cites W2136490527 @default.
- W4328104235 cites W2138207763 @default.
- W4328104235 cites W2148663332 @default.
- W4328104235 cites W2156217850 @default.
- W4328104235 cites W2179438025 @default.
- W4328104235 cites W2191758688 @default.
- W4328104235 cites W2460160187 @default.
- W4328104235 cites W2557156880 @default.
- W4328104235 cites W2572395344 @default.
- W4328104235 cites W2612155987 @default.
- W4328104235 cites W2699315399 @default.
- W4328104235 cites W2744750268 @default.
- W4328104235 cites W2767932404 @default.
- W4328104235 cites W2777173159 @default.
- W4328104235 cites W2787598078 @default.
- W4328104235 cites W2792340833 @default.
- W4328104235 cites W2883736746 @default.
- W4328104235 cites W2885319787 @default.
- W4328104235 cites W2886541918 @default.
- W4328104235 cites W2886785109 @default.
- W4328104235 cites W2893681791 @default.
- W4328104235 cites W2897889912 @default.
- W4328104235 cites W2903206977 @default.
- W4328104235 cites W2906337252 @default.
- W4328104235 cites W2917912610 @default.
- W4328104235 cites W2943135249 @default.
- W4328104235 cites W2943185083 @default.
- W4328104235 cites W2951532178 @default.
- W4328104235 cites W2951630487 @default.
- W4328104235 cites W2951912016 @default.
- W4328104235 cites W2964849163 @default.
- W4328104235 cites W2980778490 @default.
- W4328104235 cites W2990072908 @default.
- W4328104235 cites W2993820806 @default.
- W4328104235 cites W3059455360 @default.
- W4328104235 cites W3092353086 @default.
- W4328104235 cites W3109292917 @default.
- W4328104235 cites W3112765718 @default.
- W4328104235 cites W3123776742 @default.
- W4328104235 cites W3125801790 @default.
- W4328104235 cites W3129250973 @default.
- W4328104235 cites W3135055887 @default.
- W4328104235 cites W3137814608 @default.
- W4328104235 cites W3179936963 @default.
- W4328104235 cites W3183854076 @default.
- W4328104235 cites W3194665116 @default.
- W4328104235 cites W3211014030 @default.
- W4328104235 cites W3213052736 @default.
- W4328104235 cites W3215596355 @default.
- W4328104235 cites W4206722053 @default.
- W4328104235 cites W4224065213 @default.
- W4328104235 cites W4282947099 @default.
- W4328104235 cites W4283447267 @default.
- W4328104235 cites W4283587517 @default.
- W4328104235 cites W4286510410 @default.