Matches in SemOpenAlex for { <https://semopenalex.org/work/W4328114241> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W4328114241 endingPage "351" @default.
- W4328114241 startingPage "345" @default.
- W4328114241 abstract "Background: Botulinum neurotoxins (BoNTs) are among the most potent toxins known and are also used for therapeutic and aesthetic applications. Objective: An alternative in vitro cell culture bioassay based on the induction of apoptosis on T- 47D breast cancer cells, after exposure to BoNTA, was developed and validated. Methods: The T-47D cells (ATCC HTB-133) were seeded at a density of 3 × 105 cells mL-1, and the bioassay was performed with doses of BoNTA, between 3 and 81 U mL-1. The responses were assessed using 10 μL of Alamar Blue®. The absorbances were read at 570 and 600 nm. Results: The results were compared with those of the in vivo LD50 mouse bioassay, showing a non-significant 1.08% higher, mean difference of the estimated potencies (p>0.05). Besides, the biopharmaceutics is analyzed by the size exclusion and reversed-phase liquid chromatography methods, showing a significant correlation with values 1.15% higher and 0.85% lower, respectively, related to the cell culture bioassay. Conclusion: It is concluded that the validated T-47D cell culture assay represents an advancement toward the establishment of an alternative approach for the potency assessment, in the context of the 3 Rs. Besides, the employment of chromatographic methods in conjunction with the bioassays contributes to assessing the quality attributes of the biopharmaceutical formulations of BoNTA." @default.
- W4328114241 created "2023-03-22" @default.
- W4328114241 creator A5008930057 @default.
- W4328114241 creator A5020006110 @default.
- W4328114241 creator A5030608608 @default.
- W4328114241 creator A5033231303 @default.
- W4328114241 creator A5065792005 @default.
- W4328114241 date "2023-05-01" @default.
- W4328114241 modified "2023-09-26" @default.
- W4328114241 title "Validation of the T-47D Cell Culture Bioassay for the Potency Assessment of Botulinum Neurotoxin Type A" @default.
- W4328114241 cites W1975162852 @default.
- W4328114241 cites W2015795623 @default.
- W4328114241 cites W2094961834 @default.
- W4328114241 cites W2108349823 @default.
- W4328114241 cites W2110507756 @default.
- W4328114241 cites W2115295036 @default.
- W4328114241 cites W2189461777 @default.
- W4328114241 cites W2203719985 @default.
- W4328114241 cites W2416542268 @default.
- W4328114241 cites W2682693068 @default.
- W4328114241 cites W2739172437 @default.
- W4328114241 cites W2802653208 @default.
- W4328114241 cites W2804327673 @default.
- W4328114241 cites W2889977429 @default.
- W4328114241 cites W2896264863 @default.
- W4328114241 cites W2908550268 @default.
- W4328114241 cites W2992766138 @default.
- W4328114241 cites W3009632417 @default.
- W4328114241 cites W3118418919 @default.
- W4328114241 cites W3120325671 @default.
- W4328114241 cites W808994859 @default.
- W4328114241 doi "https://doi.org/10.2174/1573412919666230320155755" @default.
- W4328114241 hasPublicationYear "2023" @default.
- W4328114241 type Work @default.
- W4328114241 citedByCount "0" @default.
- W4328114241 crossrefType "journal-article" @default.
- W4328114241 hasAuthorship W4328114241A5008930057 @default.
- W4328114241 hasAuthorship W4328114241A5020006110 @default.
- W4328114241 hasAuthorship W4328114241A5030608608 @default.
- W4328114241 hasAuthorship W4328114241A5033231303 @default.
- W4328114241 hasAuthorship W4328114241A5065792005 @default.
- W4328114241 hasConcept C104488531 @default.
- W4328114241 hasConcept C150903083 @default.
- W4328114241 hasConcept C151730666 @default.
- W4328114241 hasConcept C185592680 @default.
- W4328114241 hasConcept C202751555 @default.
- W4328114241 hasConcept C207001950 @default.
- W4328114241 hasConcept C2779343474 @default.
- W4328114241 hasConcept C43617362 @default.
- W4328114241 hasConcept C54355233 @default.
- W4328114241 hasConcept C55493867 @default.
- W4328114241 hasConcept C57992300 @default.
- W4328114241 hasConcept C81885089 @default.
- W4328114241 hasConcept C86803240 @default.
- W4328114241 hasConcept C98274493 @default.
- W4328114241 hasConceptScore W4328114241C104488531 @default.
- W4328114241 hasConceptScore W4328114241C150903083 @default.
- W4328114241 hasConceptScore W4328114241C151730666 @default.
- W4328114241 hasConceptScore W4328114241C185592680 @default.
- W4328114241 hasConceptScore W4328114241C202751555 @default.
- W4328114241 hasConceptScore W4328114241C207001950 @default.
- W4328114241 hasConceptScore W4328114241C2779343474 @default.
- W4328114241 hasConceptScore W4328114241C43617362 @default.
- W4328114241 hasConceptScore W4328114241C54355233 @default.
- W4328114241 hasConceptScore W4328114241C55493867 @default.
- W4328114241 hasConceptScore W4328114241C57992300 @default.
- W4328114241 hasConceptScore W4328114241C81885089 @default.
- W4328114241 hasConceptScore W4328114241C86803240 @default.
- W4328114241 hasConceptScore W4328114241C98274493 @default.
- W4328114241 hasIssue "4" @default.
- W4328114241 hasLocation W43281142411 @default.
- W4328114241 hasOpenAccess W4328114241 @default.
- W4328114241 hasPrimaryLocation W43281142411 @default.
- W4328114241 hasRelatedWork W1982167400 @default.
- W4328114241 hasRelatedWork W2019700826 @default.
- W4328114241 hasRelatedWork W2067960249 @default.
- W4328114241 hasRelatedWork W2068050824 @default.
- W4328114241 hasRelatedWork W2119697230 @default.
- W4328114241 hasRelatedWork W2134417758 @default.
- W4328114241 hasRelatedWork W2145306431 @default.
- W4328114241 hasRelatedWork W2396932951 @default.
- W4328114241 hasRelatedWork W2800648910 @default.
- W4328114241 hasRelatedWork W2953758866 @default.
- W4328114241 hasVolume "19" @default.
- W4328114241 isParatext "false" @default.
- W4328114241 isRetracted "false" @default.
- W4328114241 workType "article" @default.