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- W4332 abstract "Nucleic acid isolation from polyphenol rich plants fail to produce good quality DNA or RNA, as polyphenols adhere and interfere with DNA during isolation. To address this problem, we at our Institute are continuously trying to improve the method for genomic DNA isolation from these plants. Use of detergents and reducing agents has resulted in identification of ascorbate as the most potent reducing agent, avoiding oxidation of polyphenols and its interference with the genomic DNA. Ascorbic acid at 40 mM concentration decreased pH of extraction buffer thereby inhibiting activity of polyphenol oxidase which converts colourless polyphenols to coloured black-brown compounds tannins and melanins. Genomic DNA obtained is superior with respect to quality and quantity to be used for analytical applications. Concentration of ascorbate above 40 mM also decreased pH of extraction buffer in the range of 4-5 causing shearing of isolated DNA competing with polyphenols to adhere the DNA." @default.
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- W4332 date "2011-08-07" @default.
- W4332 modified "2023-09-24" @default.
- W4332 title "Biochemical Role of Ascorbic acid during the Extraction of Nucleic Acids in Polyphenol Rich Medicinal Plant Tissues" @default.
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