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- W433846304 abstract "Two-dimensional gel electrophoresis practitioners have long waited for a fully automated system. This article presents an integrated platform that is capable of complete automation from sample introduction to spots detection. The strip gel for the first dimensional separation is fixed on the edge of a discrete planar stage before separation. A pair of platinum pin electrodes for isoelectric focusing (IEF) makes contact from underneath the stage. IEF is performed directly after rehydration and protein loading. After the first dimensional separation, sodium dodecyl sulfate (SDS) equilibration is done on the same stage without moving the gel. The IEF stage is then moved horizontally to couple with a precast second dimensional gel. The <0.5 mm gap between the two gels is filled with poly (ethylene oxide) solution. After SDS-polyacrylamide gel electrophoresis separation, a charge-coupled device camera is used to detect spots via protein native fluorescence excited by a Hg (Xe) lamp with the gel inside the running cell. Potential for full automation is demonstrated with 0.5 μg of Escherichia coli proteins on this miniaturized platform. More than 240 spots are detected in a total experiment time of <2.5 h. * Adapted with permission from Journal of Chromatography A, 1087(2005) 177-182 Copyright © 2005 Elsevier B.V. 2005 Elsevier B.V." @default.
- W433846304 created "2016-06-24" @default.
- W433846304 creator A5057118031 @default.
- W433846304 date "2018-08-13" @default.
- W433846304 modified "2023-09-26" @default.
- W433846304 title "Development in electrophoresis: instrumentation for two-dimensional gel electrophoresis of protein separation and application of capillary electrophoresis in micro-bioanalysis" @default.
- W433846304 doi "https://doi.org/10.31274/rtd-180813-16899" @default.
- W433846304 hasPublicationYear "2018" @default.
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