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- W434901022 abstract "An increased 2-hydroxy:16alpha-hydroxy estrogen ratio may be correlated with reduced breast cancer risk. Estrogens are hydroxylated at the C-2 position by cytochrome P450 1A (CYP1A). 2-Hydroxyestrogens can be methylated by catechol-O-methyltransferase to form antitumorigenic 2-methoxyestrogens, or can be oxidized to quinones that may react with DNA. Quinones may be reduced back to 2-hydroxyestrogens by NAD(P)H:quinone oxidoreductase (NQO1). Broccoli contains glucosinolates that, upon hydrolysis, form bioactive isothiocyanates, which are known inducers of CYP1A and NQO1. Aqueous extracts of freeze-dried broccoli powders were generated through overnight hydrolysis at room temperature (50 mg/mL water). Incubation of mouse hepatoma Hepa-1c1c7 cells with broccoli extracts for 24 h induced CYP1A and NQO1 activities up to 72 and 4.9-fold, respectively. When Hepa-1c1c7 cells were incubated with 10 μM estradiol in the absence of broccoli extracts, no 2-methoxyestradiol formation was observed by HPLC-MS/MS. In contrast, a 24 h exposure of cells to broccoli extracts, followed by 24 h simultaneous exposure to broccoli extracts and 10 μM estradiol lead to formation of measurable amounts of 2-methoxyestradiol in the culture medium. These results indicate that broccoli may redirect estrogen metabolism toward formation of anti-tumorigenic 2-methoxyestradiol, through induction of CYP1A and NQO1 activity. (supported by grants from USDA and NIH)" @default.
- W434901022 created "2016-06-24" @default.
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- W434901022 date "2010-04-01" @default.
- W434901022 modified "2023-09-27" @default.
- W434901022 title "Broccoli alters estrogen metabolism in favor of 2‐methoxyestradiol in vitro" @default.
- W434901022 doi "https://doi.org/10.1096/fasebj.24.1_supplement.720.5" @default.
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