FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4353067647> ?p ?o ?g. }

• W4353067647 endingPage "251" @default.
• W4353067647 startingPage "251" @default.
• W4353067647 abstract "Marine gel particles (MGP) are amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans, but their biochemical composition and function are poorly understood. While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP). Previous studies targeted MGPs isolated by filtration. We developed a new way of isolating MGPs from seawater in liquid suspension and applied it to identify extracellular DNA (eDNA) in North Sea surface seawater. Seawater was filtered onto polycarbonate (PC) filters with gentle vacuum filtration, and then the filtered particles were gently resuspended in a smaller volume of sterile seawater. The resulting MGPs ranged in size from 0.4 to 100 µm in diameter. eDNA was detected by fluorescent microscopy using YOYO-1 (for eDNA), with Nile red (targeting cell membranes) as a counterstain. TOTO-3 was also used to stain eDNA, with ConA to localise glycoproteins and SYTO-9 for the live/dead staining of cells. Confocal laser scanning microscopy (CLSM) revealed the presence of proteins and polysaccharides. We found eDNA to be universally associated with MGPs. To further elucidate the role of eDNA, we established a model experimental MGP system using bacterial EPS from Pseudoalteromonas atlantica that also contained eDNA. Our results clearly demonstrate the occurrence of eDNA in MGPs, and should aid furthering our understanding of the micro-scale dynamics and fate of MGPs that underly the large-scale processes of carbon cycling and sedimentation in the ocean." @default.
• W4353067647 created "2023-03-23" @default.
• W4353067647 creator A5046093816 @default.
• W4353067647 creator A5073811999 @default.
• W4353067647 creator A5086327901 @default.
• W4353067647 date "2023-03-21" @default.
• W4353067647 modified "2023-09-27" @default.
• W4353067647 title "Isolation and Staining Reveal the Presence of Extracellular DNA in Marine Gel Particles" @default.
• W4353067647 cites W1481525432 @default.
• W4353067647 cites W1486037013 @default.
• W4353067647 cites W1492254333 @default.
• W4353067647 cites W1546703330 @default.
• W4353067647 cites W1572824337 @default.
• W4353067647 cites W1602765691 @default.
• W4353067647 cites W1761265770 @default.
• W4353067647 cites W1939674419 @default.
• W4353067647 cites W1963539735 @default.
• W4353067647 cites W1963790466 @default.
• W4353067647 cites W1965526506 @default.
• W4353067647 cites W1967301341 @default.
• W4353067647 cites W1968459201 @default.
• W4353067647 cites W1975243918 @default.
• W4353067647 cites W1977384597 @default.
• W4353067647 cites W1987588611 @default.
• W4353067647 cites W1991267907 @default.
• W4353067647 cites W1995692755 @default.
• W4353067647 cites W2001070837 @default.
• W4353067647 cites W2007215538 @default.
• W4353067647 cites W2016969072 @default.
• W4353067647 cites W2023981239 @default.
• W4353067647 cites W2026538249 @default.
• W4353067647 cites W2027438571 @default.
• W4353067647 cites W2029388535 @default.
• W4353067647 cites W2032593299 @default.
• W4353067647 cites W2036616320 @default.
• W4353067647 cites W2038070685 @default.
• W4353067647 cites W2047299075 @default.
• W4353067647 cites W2050814258 @default.
• W4353067647 cites W2052759556 @default.
• W4353067647 cites W2057137147 @default.
• W4353067647 cites W2057658553 @default.
• W4353067647 cites W2058486476 @default.
• W4353067647 cites W2061517309 @default.
• W4353067647 cites W2064612830 @default.
• W4353067647 cites W2071594970 @default.
• W4353067647 cites W2081160511 @default.
• W4353067647 cites W2084278313 @default.
• W4353067647 cites W2094026803 @default.
• W4353067647 cites W2095082323 @default.
• W4353067647 cites W2095940426 @default.
• W4353067647 cites W2105466401 @default.
• W4353067647 cites W2129622024 @default.
• W4353067647 cites W2131347959 @default.
• W4353067647 cites W2131511954 @default.
• W4353067647 cites W2133617587 @default.
• W4353067647 cites W2145810508 @default.
• W4353067647 cites W2145845793 @default.
• W4353067647 cites W2156382695 @default.
• W4353067647 cites W2160575983 @default.
• W4353067647 cites W2283208739 @default.
• W4353067647 cites W2296525274 @default.
• W4353067647 cites W2333059703 @default.
• W4353067647 cites W2340571531 @default.
• W4353067647 cites W2342598290 @default.
• W4353067647 cites W2534348335 @default.
• W4353067647 cites W2537496006 @default.
• W4353067647 cites W2552060483 @default.
• W4353067647 cites W2571248300 @default.
• W4353067647 cites W2589905973 @default.
• W4353067647 cites W2616935735 @default.
• W4353067647 cites W2623874444 @default.
• W4353067647 cites W2624222859 @default.
• W4353067647 cites W2784078401 @default.
• W4353067647 cites W2788450648 @default.
• W4353067647 cites W2802928286 @default.
• W4353067647 cites W2807272898 @default.
• W4353067647 cites W2887615907 @default.
• W4353067647 cites W2921331759 @default.
• W4353067647 cites W2932803502 @default.
• W4353067647 cites W2944501980 @default.
• W4353067647 cites W2949959694 @default.
• W4353067647 cites W2999421358 @default.
• W4353067647 cites W3022516180 @default.
• W4353067647 cites W3083657241 @default.
• W4353067647 cites W3121425301 @default.
• W4353067647 cites W3137896275 @default.
• W4353067647 cites W3155144384 @default.
• W4353067647 cites W3189984932 @default.
• W4353067647 cites W3193011163 @default.
• W4353067647 cites W3208518864 @default.
• W4353067647 cites W4224254881 @default.
• W4353067647 cites W4239687103 @default.
• W4353067647 cites W4281729401 @default.
• W4353067647 doi "https://doi.org/10.3390/gels9030251" @default.
• W4353067647 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36975700" @default.
• W4353067647 hasPublicationYear "2023" @default.
• W4353067647 type Work @default.
• W4353067647 citedByCount "0" @default.

• next