Matches in SemOpenAlex for { <https://semopenalex.org/work/W4360603356> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W4360603356 endingPage "803" @default.
- W4360603356 startingPage "793" @default.
- W4360603356 abstract "Autoimmune factor XIII (FXIII) deficiency (AiF13D) is an acquired life-threatening bleeding disorder due to anti-FXIII autoantibodies (autoAbs). We previously established an immunochromatographic test (ICT) for detection of anti-FXIII-A subunit (FXIII-A) autoAbs. Conversely, the detection of anti-FXIII-B subunit (FXIII-B) autoAbs is currently performed in a limited number of medical facilities through time-consuming and expensive laboratory tests, such as dot-blotting analysis and enzyme-linked immunosorbent assay (ELISA). Accordingly, in this study, we generated eight rat monoclonal antibodies (mAbs) against human FXIII-B using the rat lymph node method. By employing an ELISA, two mAbs, 2G12B10 and 8H12B9, were selected considering the distance between the recognition regions of each mAb (the 6th and 9th-10th Sushi domain, respectively) and the strength of their reactivity. Using this mAb combination, we prototyped an ICT to detect anti-FXIII-B autoAbs and distinguish between AiF13D and nonimmune acquired FXIII deficiency (acF13D), and tested it with 22 healthy controls, 23 acF13D patients, 15 AiF13D patients without anti-FXIII-B autoAbs, and 8 AiF13D patients with anti-FXIII-B autoAbs. Receiver operating characteristic curve analyses of ICTs for anti-FXIII-B autoAbs were performed and revealed a precision similar to dot-blot analysis. Human anti-FXIII-A mAbs were also generated from a single patient with AiF13D using a new cDNA cloning method, and their binding properties were characterized. Consequently, anti-FXIII-A immunoglobulin G preparations were established as potentially permanent positive controls of ICT for anti-FXIII-A antibodies. Combining the previously developed ICT for anti-FXIII-A autoAbs and the novel ICT for anti-FXIII-B autoAbs may reduce false negatives and lead to appropriate diagnosis and treatment." @default.
- W4360603356 created "2023-03-24" @default.
- W4360603356 creator A5019792028 @default.
- W4360603356 creator A5053065921 @default.
- W4360603356 creator A5064588827 @default.
- W4360603356 creator A5066240538 @default.
- W4360603356 creator A5083238091 @default.
- W4360603356 date "2023-03-23" @default.
- W4360603356 modified "2023-09-29" @default.
- W4360603356 title "Novel Immunochromatographic Test for Anti-factor XIII B Subunit Autoantibodies to Diagnose Autoimmune Acquired Factor XIII Deficiency" @default.
- W4360603356 cites W1488919497 @default.
- W4360603356 cites W1527634600 @default.
- W4360603356 cites W1974343039 @default.
- W4360603356 cites W1977955584 @default.
- W4360603356 cites W1980527619 @default.
- W4360603356 cites W1990748933 @default.
- W4360603356 cites W2023659116 @default.
- W4360603356 cites W2055914175 @default.
- W4360603356 cites W2069062911 @default.
- W4360603356 cites W2069379572 @default.
- W4360603356 cites W2070333880 @default.
- W4360603356 cites W2090949044 @default.
- W4360603356 cites W2091202677 @default.
- W4360603356 cites W2116107442 @default.
- W4360603356 cites W2149170225 @default.
- W4360603356 cites W2152723000 @default.
- W4360603356 cites W2321654092 @default.
- W4360603356 cites W2342264870 @default.
- W4360603356 cites W2483649712 @default.
- W4360603356 cites W2507339732 @default.
- W4360603356 cites W2615822817 @default.
- W4360603356 cites W2792344011 @default.
- W4360603356 cites W2950248779 @default.
- W4360603356 cites W3136931257 @default.
- W4360603356 cites W3153274319 @default.
- W4360603356 cites W4313730606 @default.
- W4360603356 doi "https://doi.org/10.1055/a-2061-3182" @default.
- W4360603356 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36958345" @default.
- W4360603356 hasPublicationYear "2023" @default.
- W4360603356 type Work @default.
- W4360603356 citedByCount "0" @default.
- W4360603356 crossrefType "journal-article" @default.
- W4360603356 hasAuthorship W4360603356A5019792028 @default.
- W4360603356 hasAuthorship W4360603356A5053065921 @default.
- W4360603356 hasAuthorship W4360603356A5064588827 @default.
- W4360603356 hasAuthorship W4360603356A5066240538 @default.
- W4360603356 hasAuthorship W4360603356A5083238091 @default.
- W4360603356 hasConcept C153911025 @default.
- W4360603356 hasConcept C159654299 @default.
- W4360603356 hasConcept C163764329 @default.
- W4360603356 hasConcept C203014093 @default.
- W4360603356 hasConcept C2779243652 @default.
- W4360603356 hasConcept C2779672106 @default.
- W4360603356 hasConcept C54173615 @default.
- W4360603356 hasConcept C542903549 @default.
- W4360603356 hasConcept C71924100 @default.
- W4360603356 hasConcept C86803240 @default.
- W4360603356 hasConceptScore W4360603356C153911025 @default.
- W4360603356 hasConceptScore W4360603356C159654299 @default.
- W4360603356 hasConceptScore W4360603356C163764329 @default.
- W4360603356 hasConceptScore W4360603356C203014093 @default.
- W4360603356 hasConceptScore W4360603356C2779243652 @default.
- W4360603356 hasConceptScore W4360603356C2779672106 @default.
- W4360603356 hasConceptScore W4360603356C54173615 @default.
- W4360603356 hasConceptScore W4360603356C542903549 @default.
- W4360603356 hasConceptScore W4360603356C71924100 @default.
- W4360603356 hasConceptScore W4360603356C86803240 @default.
- W4360603356 hasFunder F4320311405 @default.
- W4360603356 hasFunder F4320320912 @default.
- W4360603356 hasIssue "08" @default.
- W4360603356 hasLocation W43606033561 @default.
- W4360603356 hasLocation W43606033562 @default.
- W4360603356 hasOpenAccess W4360603356 @default.
- W4360603356 hasPrimaryLocation W43606033561 @default.
- W4360603356 hasRelatedWork W1973692620 @default.
- W4360603356 hasRelatedWork W1987381631 @default.
- W4360603356 hasRelatedWork W2023669480 @default.
- W4360603356 hasRelatedWork W2063428357 @default.
- W4360603356 hasRelatedWork W2101875571 @default.
- W4360603356 hasRelatedWork W2368460920 @default.
- W4360603356 hasRelatedWork W2380360044 @default.
- W4360603356 hasRelatedWork W2394899461 @default.
- W4360603356 hasRelatedWork W2425255793 @default.
- W4360603356 hasRelatedWork W3045746510 @default.
- W4360603356 hasVolume "123" @default.
- W4360603356 isParatext "false" @default.
- W4360603356 isRetracted "false" @default.
- W4360603356 workType "article" @default.