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- W4360611383 abstract "Genome assembly into chromosomes facilitates several analyses including cytogenetics, genomics and phylogenetics. Despite rapid development in bioinformatics, however, assembly beyond scaffolds remains challenging, especially in species without closely related well-assembled and available reference genomes. So far, four draft genomes of Rangifer tarandus (caribou or reindeer, a circumpolar distributed cervid species) have been published, but none with chromosome-level assembly. This emblematic northern species is of high interest in ecological studies and conservation since most populations are declining.We have designed specific probes based on Oligopaint FISH technology to upgrade the latest published reindeer and caribou chromosome-level genomes. Using this oligonucleotide-based method, we found six mis-assembled scaffolds and physically mapped 68 of the largest scaffolds representing 78% of the most recent R. tarandus genome assembly. Combining physical mapping and comparative genomics, it was possible to document chromosomal evolution among Cervidae and closely related bovids.Our results provide validation for the current chromosome-level genome assembly as well as resources to use chromosome banding in studies of Rangifer tarandus." @default.
- W4360611383 created "2023-03-24" @default.
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- W4360611383 date "2023-03-23" @default.
- W4360611383 modified "2023-10-09" @default.
- W4360611383 title "Chromosome-level assembly of the Rangifer tarandus genome and validation of cervid and bovid evolution insights" @default.
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- W4360611383 doi "https://doi.org/10.1186/s12864-023-09189-5" @default.
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- W4360611383 hasPublicationYear "2023" @default.
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