Matches in SemOpenAlex for { <https://semopenalex.org/work/W4360823197> ?p ?o ?g. }
- W4360823197 abstract "Abstract Calcium in interstitial fluids is central to systemic physiology and a crucial ion pool for entry into cells through numerous plasma membrane channels. Its study has been limited by the lack of methods that allow monitoring in tight inter-cell spaces at high spatio-temporal resolution. We engineered high performance ultra-low affinity genetically encoded calcium biosensors named GreenT-ECs. GreenT-ECs combine large fluorescence changes upon calcium binding and binding affinities (K D ) ranging from 0.8 mM to 2.9 mM, making them uniquely tuned to calcium concentrations in extracellular organismal fluids. We validated GreenT-ECs in rodent hippocampal neurons and transgenic zebrafish in vivo , where the sensors enabled monitoring homeostatic regulation of tissue interstitial calcium. GreenT-ECs may become useful for recording very large calcium transients and for imaging calcium homeostasis in inter-cell structures in live tissues and organisms." @default.
- W4360823197 created "2023-03-25" @default.
- W4360823197 creator A5011362603 @default.
- W4360823197 creator A5021234976 @default.
- W4360823197 creator A5026734803 @default.
- W4360823197 creator A5027092747 @default.
- W4360823197 creator A5039718992 @default.
- W4360823197 creator A5040211959 @default.
- W4360823197 creator A5069221051 @default.
- W4360823197 creator A5073047847 @default.
- W4360823197 creator A5086150959 @default.
- W4360823197 date "2023-03-24" @default.
- W4360823197 modified "2023-10-06" @default.
- W4360823197 title "Fluorescent Sensors for Imaging Interstitial Calcium" @default.
- W4360823197 cites W1484685406 @default.
- W4360823197 cites W1542263802 @default.
- W4360823197 cites W1571164133 @default.
- W4360823197 cites W1599277155 @default.
- W4360823197 cites W1599670156 @default.
- W4360823197 cites W1976136773 @default.
- W4360823197 cites W1987278178 @default.
- W4360823197 cites W1996761102 @default.
- W4360823197 cites W2003108264 @default.
- W4360823197 cites W2006176357 @default.
- W4360823197 cites W2009441758 @default.
- W4360823197 cites W2009766418 @default.
- W4360823197 cites W2022889543 @default.
- W4360823197 cites W2028827586 @default.
- W4360823197 cites W2061463538 @default.
- W4360823197 cites W2066777402 @default.
- W4360823197 cites W2067678773 @default.
- W4360823197 cites W2078290510 @default.
- W4360823197 cites W2079054696 @default.
- W4360823197 cites W2080092859 @default.
- W4360823197 cites W2085556812 @default.
- W4360823197 cites W2089247240 @default.
- W4360823197 cites W2099021265 @default.
- W4360823197 cites W2106856165 @default.
- W4360823197 cites W2125728751 @default.
- W4360823197 cites W2149161770 @default.
- W4360823197 cites W2150242497 @default.
- W4360823197 cites W2152230385 @default.
- W4360823197 cites W2157199084 @default.
- W4360823197 cites W2168080864 @default.
- W4360823197 cites W2336542768 @default.
- W4360823197 cites W2344367297 @default.
- W4360823197 cites W2539831119 @default.
- W4360823197 cites W2544939113 @default.
- W4360823197 cites W2551836638 @default.
- W4360823197 cites W2890873632 @default.
- W4360823197 cites W2897807700 @default.
- W4360823197 cites W2901644191 @default.
- W4360823197 cites W2936067526 @default.
- W4360823197 cites W2955893057 @default.
- W4360823197 cites W2959162700 @default.
- W4360823197 cites W2972662355 @default.
- W4360823197 cites W2980605119 @default.
- W4360823197 cites W3127598249 @default.
- W4360823197 cites W3145094704 @default.
- W4360823197 cites W3188300656 @default.
- W4360823197 cites W3193463113 @default.
- W4360823197 cites W3211783136 @default.
- W4360823197 cites W4224951780 @default.
- W4360823197 cites W4294990909 @default.
- W4360823197 cites W4361960923 @default.
- W4360823197 doi "https://doi.org/10.1101/2023.03.23.533956" @default.
- W4360823197 hasPublicationYear "2023" @default.
- W4360823197 type Work @default.
- W4360823197 citedByCount "0" @default.
- W4360823197 crossrefType "posted-content" @default.
- W4360823197 hasAuthorship W4360823197A5011362603 @default.
- W4360823197 hasAuthorship W4360823197A5021234976 @default.
- W4360823197 hasAuthorship W4360823197A5026734803 @default.
- W4360823197 hasAuthorship W4360823197A5027092747 @default.
- W4360823197 hasAuthorship W4360823197A5039718992 @default.
- W4360823197 hasAuthorship W4360823197A5040211959 @default.
- W4360823197 hasAuthorship W4360823197A5069221051 @default.
- W4360823197 hasAuthorship W4360823197A5073047847 @default.
- W4360823197 hasAuthorship W4360823197A5086150959 @default.
- W4360823197 hasBestOaLocation W43608231971 @default.
- W4360823197 hasConcept C104317684 @default.
- W4360823197 hasConcept C121332964 @default.
- W4360823197 hasConcept C12554922 @default.
- W4360823197 hasConcept C1491633281 @default.
- W4360823197 hasConcept C15526883 @default.
- W4360823197 hasConcept C178790620 @default.
- W4360823197 hasConcept C185592680 @default.
- W4360823197 hasConcept C201571599 @default.
- W4360823197 hasConcept C2776032975 @default.
- W4360823197 hasConcept C2776878037 @default.
- W4360823197 hasConcept C28406088 @default.
- W4360823197 hasConcept C519063684 @default.
- W4360823197 hasConcept C55493867 @default.
- W4360823197 hasConcept C62520636 @default.
- W4360823197 hasConcept C63645605 @default.
- W4360823197 hasConcept C86803240 @default.
- W4360823197 hasConcept C87975464 @default.
- W4360823197 hasConcept C91881484 @default.
- W4360823197 hasConcept C95444343 @default.
- W4360823197 hasConceptScore W4360823197C104317684 @default.