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- W4360836974 endingPage "e14624" @default.
- W4360836974 startingPage "e14624" @default.
- W4360836974 abstract "MLO (mildew locus O) genes play a vital role in plant disease defense system, especially powdery mildew (PM). Lagenaria siceraria is a distinct Cucurbitaceae crop, and PM is one of the most serious diseases threatening crop production and quality. Although MLOs have been exploited in many Cucurbitaceae species, genome-wide mining of MLO gene family in bottle gourd has not been surveyed yet. Here we identified 16 MLO genes in our recently assembled L. siceraria genome. A total of 343 unique MLO protein sequences from 20 species were characterized and compared to deduce a generally high level of purifying selection and the occurrence of regions related to candidate susceptibility factors in the evolutional divergence. LsMLOs were clustered in six clades containing seven conserved transmembrane domains and 10 clade-specific motifs along with deletion and variation. Three genes (LsMLO3, LsMLO6, and LsMLO13) in clade V showed high sequence identity with orthologues involved in PM susceptibility. The expression pattern of LsMLOs was tissue-specific but not cultivar-specific. Furthermore, it was indicated by qRT-PCR and RNA-seq that LsMLO3 and LsMLO13 were highly upregulated in response to PM stress. Subsequent sequence analysis revealed the structural deletion of LsMLO13 and a single nonsynonymous substitution of LsMLO3 in the PM-resistant genotype. Taken all together, it is speculated that LsMLO13 is likely a major PM susceptibility factor. The results of this study provide new insights into MLO family genes in bottle gourd and find a potential candidate S gene for PM tolerance breeding." @default.
- W4360836974 created "2023-03-25" @default.
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- W4360836974 date "2023-04-01" @default.
- W4360836974 modified "2023-10-15" @default.
- W4360836974 title "Genome-wide characterization and expression analysis of the MLO gene family sheds light on powdery mildew resistance in Lagenaria siceraria" @default.
- W4360836974 cites W1497493092 @default.
- W4360836974 cites W1519105983 @default.
- W4360836974 cites W1928252133 @default.
- W4360836974 cites W1963549704 @default.
- W4360836974 cites W1972004632 @default.
- W4360836974 cites W1979522585 @default.
- W4360836974 cites W1980201684 @default.
- W4360836974 cites W1996439449 @default.
- W4360836974 cites W2006838963 @default.
- W4360836974 cites W2014405514 @default.
- W4360836974 cites W2016538425 @default.
- W4360836974 cites W2022525179 @default.
- W4360836974 cites W2028510381 @default.
- W4360836974 cites W2029937437 @default.
- W4360836974 cites W2030707763 @default.
- W4360836974 cites W2031182595 @default.
- W4360836974 cites W2041386815 @default.
- W4360836974 cites W2043011797 @default.
- W4360836974 cites W2054557417 @default.
- W4360836974 cites W2055411819 @default.
- W4360836974 cites W2056026865 @default.
- W4360836974 cites W2062665697 @default.
- W4360836974 cites W2063055663 @default.
- W4360836974 cites W2066194277 @default.
- W4360836974 cites W2073386273 @default.
- W4360836974 cites W2075822346 @default.
- W4360836974 cites W2083529822 @default.
- W4360836974 cites W2086208654 @default.
- W4360836974 cites W2094662908 @default.
- W4360836974 cites W2097870001 @default.
- W4360836974 cites W2106098031 @default.
- W4360836974 cites W2108116413 @default.
- W4360836974 cites W2111647009 @default.
- W4360836974 cites W2113559899 @default.
- W4360836974 cites W2114425248 @default.
- W4360836974 cites W2120772351 @default.
- W4360836974 cites W2122199454 @default.
- W4360836974 cites W2140307684 @default.
- W4360836974 cites W2147159068 @default.
- W4360836974 cites W2157009395 @default.
- W4360836974 cites W2158349294 @default.
- W4360836974 cites W2158565983 @default.
- W4360836974 cites W2165898164 @default.
- W4360836974 cites W2166717297 @default.
- W4360836974 cites W2169288593 @default.
- W4360836974 cites W2171918592 @default.
- W4360836974 cites W2187745401 @default.
- W4360836974 cites W2210787609 @default.
- W4360836974 cites W2257046203 @default.
- W4360836974 cites W2258762858 @default.
- W4360836974 cites W2294763389 @default.
- W4360836974 cites W2311203695 @default.
- W4360836974 cites W2312085627 @default.
- W4360836974 cites W2318211409 @default.
- W4360836974 cites W2345123064 @default.
- W4360836974 cites W2418413180 @default.
- W4360836974 cites W2520038174 @default.
- W4360836974 cites W2523007828 @default.
- W4360836974 cites W2558131312 @default.
- W4360836974 cites W2567243116 @default.
- W4360836974 cites W2598396806 @default.
- W4360836974 cites W2603054892 @default.
- W4360836974 cites W2606442475 @default.
- W4360836974 cites W2619837469 @default.
- W4360836974 cites W2760233468 @default.
- W4360836974 cites W2781938185 @default.
- W4360836974 cites W2801983997 @default.
- W4360836974 cites W2803002795 @default.
- W4360836974 cites W2847206774 @default.
- W4360836974 cites W3015663661 @default.
- W4360836974 cites W3036319615 @default.
- W4360836974 cites W3106665119 @default.
- W4360836974 cites W3138235794 @default.
- W4360836974 cites W3156458251 @default.
- W4360836974 cites W3157312319 @default.
- W4360836974 cites W3163172504 @default.
- W4360836974 cites W3164307178 @default.
- W4360836974 cites W3195263129 @default.
- W4360836974 cites W4205459347 @default.
- W4360836974 doi "https://doi.org/10.1016/j.heliyon.2023.e14624" @default.
- W4360836974 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37025859" @default.
- W4360836974 hasPublicationYear "2023" @default.
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