FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4360950949> ?p ?o ?g. }

• W4360950949 abstract "Abstract The split-Gal4 system allows for intersectional genetic labeling of highly specific cell-types and tissues in Drosophila . However, the existing split-Gal4 system, unlike the standard Gal4 system, cannot be repressed by Gal80, and therefore cannot be controlled temporally. This lack of temporal control precludes split-Gal4 experiments in which a genetic manipulation must be restricted to specific timepoints. Here, we describe a new split-Gal4 system based on a self-excising split-intein, which drives transgene expression as strongly as the current split-Gal4 system and Gal4 reagents, yet which is fully repressible by Gal80. We demonstrate the potent inducibility of “split-intein Gal4” in vivo using both fluorescent reporters and via reversible tumor induction in the gut. Further, we show that our split-intein Gal4 can be extended to the drug-inducible GeneSwitch system, providing an independent method for intersectional labeling with inducible control. We also show that the split-intein Gal4 system can be used to generate highly cell-type specific genetic drivers based on in silico predictions generated by single cell RNAseq (scRNAseq) datasets, and we describe a new algorithm (“Two Against Background” or TAB) to predict cluster-specific gene pairs across multiple tissue-specific scRNA datasets. We provide a plasmid toolkit to efficiently create split-intein Gal4 drivers based on either CRISPR knock-ins to target genes or using enhancer fragments. Altogether, the split-intein Gal4 system allows for the creation of highly specific intersectional genetic drivers that are inducible/repressible. Significance statement The split-Gal4 system allows Drosophila researchers to drive transgene expression with extraordinary cell type specificity. However, the existing split-Gal4 system cannot be controlled temporally, and therefore cannot be applied to many important areas of research. Here, we present a new split-Gal4 system based on a self-excising split-intein, which is fully controllable by Gal80, as well as a related drug-inducible split GeneSwitch system. This approach can both leverage and inform single-cell RNAseq datasets, and we introduce an algorithm to identify pairs of genes that precisely and narrowly mark a desired cell cluster. Our split-intein Gal4 system will be of value to the Drosophila research community, and allow for the creation of highly specific genetic drivers that are also inducible/repressible." @default.
• W4360950949 created "2023-03-26" @default.
• W4360950949 creator A5002750169 @default.
• W4360950949 creator A5013955099 @default.
• W4360950949 creator A5016952505 @default.
• W4360950949 creator A5022944276 @default.
• W4360950949 creator A5044078921 @default.
• W4360950949 creator A5071083794 @default.
• W4360950949 creator A5081688191 @default.
• W4360950949 creator A5083951258 @default.
• W4360950949 creator A5084845938 @default.
• W4360950949 date "2023-03-24" @default.
• W4360950949 modified "2023-10-16" @default.
• W4360950949 title "split-intein Gal4 provides intersectional genetic labeling that is fully repressible by Gal80" @default.
• W4360950949 cites W1008499258 @default.
• W4360950949 cites W1968814136 @default.
• W4360950949 cites W1971897583 @default.
• W4360950949 cites W1975712471 @default.
• W4360950949 cites W1978157456 @default.
• W4360950949 cites W1984841747 @default.
• W4360950949 cites W1998356880 @default.
• W4360950949 cites W2006741453 @default.
• W4360950949 cites W2009181661 @default.
• W4360950949 cites W2021892293 @default.
• W4360950949 cites W2035478311 @default.
• W4360950949 cites W2035505151 @default.
• W4360950949 cites W2037598426 @default.
• W4360950949 cites W2040734916 @default.
• W4360950949 cites W2056558785 @default.
• W4360950949 cites W2057050252 @default.
• W4360950949 cites W2066027084 @default.
• W4360950949 cites W2070386387 @default.
• W4360950949 cites W2079657320 @default.
• W4360950949 cites W2081092282 @default.
• W4360950949 cites W2088960975 @default.
• W4360950949 cites W2092098765 @default.
• W4360950949 cites W2095729063 @default.
• W4360950949 cites W2110643873 @default.
• W4360950949 cites W2122806269 @default.
• W4360950949 cites W2132605861 @default.
• W4360950949 cites W2142018200 @default.
• W4360950949 cites W2153013018 @default.
• W4360950949 cites W2157071511 @default.
• W4360950949 cites W2170501485 @default.
• W4360950949 cites W2343742479 @default.
• W4360950949 cites W2516024337 @default.
• W4360950949 cites W2737841898 @default.
• W4360950949 cites W2761788149 @default.
• W4360950949 cites W2765915208 @default.
• W4360950949 cites W2791843246 @default.
• W4360950949 cites W2985493807 @default.
• W4360950949 cites W2986502034 @default.
• W4360950949 cites W2999857418 @default.
• W4360950949 cites W3016127948 @default.
• W4360950949 cites W3084421525 @default.
• W4360950949 cites W3088010708 @default.
• W4360950949 cites W3106447471 @default.
• W4360950949 cites W3165128404 @default.
• W4360950949 cites W3199465610 @default.
• W4360950949 cites W4214861448 @default.
• W4360950949 cites W4220669594 @default.
• W4360950949 cites W4225137572 @default.
• W4360950949 cites W4283168311 @default.
• W4360950949 cites W4319165003 @default.
• W4360950949 doi "https://doi.org/10.1101/2023.03.24.534001" @default.
• W4360950949 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36993523" @default.
• W4360950949 hasPublicationYear "2023" @default.
• W4360950949 type Work @default.
• W4360950949 citedByCount "1" @default.
• W4360950949 countsByYear W43609509492023 @default.
• W4360950949 crossrefType "posted-content" @default.
• W4360950949 hasAuthorship W4360950949A5002750169 @default.
• W4360950949 hasAuthorship W4360950949A5013955099 @default.
• W4360950949 hasAuthorship W4360950949A5016952505 @default.
• W4360950949 hasAuthorship W4360950949A5022944276 @default.
• W4360950949 hasAuthorship W4360950949A5044078921 @default.
• W4360950949 hasAuthorship W4360950949A5071083794 @default.
• W4360950949 hasAuthorship W4360950949A5081688191 @default.
• W4360950949 hasAuthorship W4360950949A5083951258 @default.
• W4360950949 hasAuthorship W4360950949A5084845938 @default.
• W4360950949 hasBestOaLocation W43609509491 @default.
• W4360950949 hasConcept C102230213 @default.
• W4360950949 hasConcept C104317684 @default.
• W4360950949 hasConcept C111936080 @default.
• W4360950949 hasConcept C125929170 @default.
• W4360950949 hasConcept C141035611 @default.
• W4360950949 hasConcept C150194340 @default.
• W4360950949 hasConcept C2775954774 @default.
• W4360950949 hasConcept C54355233 @default.
• W4360950949 hasConcept C54458228 @default.
• W4360950949 hasConcept C67705224 @default.
• W4360950949 hasConcept C70721500 @default.
• W4360950949 hasConcept C86803240 @default.
• W4360950949 hasConcept C98108389 @default.
• W4360950949 hasConceptScore W4360950949C102230213 @default.
• W4360950949 hasConceptScore W4360950949C104317684 @default.
• W4360950949 hasConceptScore W4360950949C111936080 @default.
• W4360950949 hasConceptScore W4360950949C125929170 @default.
• W4360950949 hasConceptScore W4360950949C141035611 @default.
• W4360950949 hasConceptScore W4360950949C150194340 @default.

• next