Matches in SemOpenAlex for { <https://semopenalex.org/work/W4360953814> ?p ?o ?g. }
Showing items 1 to 58 of
58
with 100 items per page.
- W4360953814 endingPage "9" @default.
- W4360953814 startingPage "3" @default.
- W4360953814 abstract "Proteome-based vegetative and flower bud formation characterization was utilized to identify and differentiate protein species with significant variable abundance during floral transition in mango cv. Dashehari using 2DE and corroborated the identified protein spots using gene expression analysis. Total soluble proteins were phenol-extracted from mango cv's vegetative and floral flush. Dashehari and separated on 2D gels at pH 4-7. The protein spots with variable intensity were identified through SameSpots software. The protein sequences of differentially accumulated spots were identified based on PI and MW using Citrus sinensis proteome isoelectric focusing database. Furthermore, these protein sequences were used to conduct (tBLASTn) against Mangifera indica to predict the protein. Real time gene expression was done to corroborate identified proteins. Total 301 spots were detected, out of which 16 were identified as differentially expressed (P?0.05) and a 2-fold change. These 16 protein spots were identified on the basis of in silico comparative mapping protein against genome of mango and citrus, a close relative. They were classified into eight categories: transcriptional regulation, phenylpropanoid pathway and cell wall /cytoskeleton metabolism-related proteins, hormone signalling, flowering time, signal-transduction, transport and protein synthesis to flowering. Five genes coding for shortlisted proteins were used for validation of results using gene expression analysis. SAM (S adenosyl methonine synthase) was found up-regulated in floral flush, involved in the biosynthesis of polyamines has association with flowering, and stress responses. Furthermore, ARF (Auxin Response Factor), serine/threonine kinase gene members were also found to play critical role in determining floral development process in mango, consistent with results obtained through 2DE. Protein species that are putatively involved in phenylpropanoid pathway were also identified, showing the process of mango flowering from a new perspective beyond the conventional view. This flowering related proteomics study provides an overview of the biological pathways and regulatory mechanisms associated with flowering developmental physiology." @default.
- W4360953814 created "2023-03-26" @default.
- W4360953814 creator A5025947309 @default.
- W4360953814 creator A5049132048 @default.
- W4360953814 creator A5063530569 @default.
- W4360953814 creator A5065340348 @default.
- W4360953814 creator A5078913654 @default.
- W4360953814 creator A5081683129 @default.
- W4360953814 date "2023-03-10" @default.
- W4360953814 modified "2023-09-30" @default.
- W4360953814 title "Proteomic landscape presents cues for vegetative to reproductive transition in mango" @default.
- W4360953814 doi "https://doi.org/10.37855/jah.2023.v25i01.01" @default.
- W4360953814 hasPublicationYear "2023" @default.
- W4360953814 type Work @default.
- W4360953814 citedByCount "0" @default.
- W4360953814 crossrefType "journal-article" @default.
- W4360953814 hasAuthorship W4360953814A5025947309 @default.
- W4360953814 hasAuthorship W4360953814A5049132048 @default.
- W4360953814 hasAuthorship W4360953814A5063530569 @default.
- W4360953814 hasAuthorship W4360953814A5065340348 @default.
- W4360953814 hasAuthorship W4360953814A5078913654 @default.
- W4360953814 hasAuthorship W4360953814A5081683129 @default.
- W4360953814 hasConcept C104317684 @default.
- W4360953814 hasConcept C104397665 @default.
- W4360953814 hasConcept C150194340 @default.
- W4360953814 hasConcept C2781255879 @default.
- W4360953814 hasConcept C2781429666 @default.
- W4360953814 hasConcept C54355233 @default.
- W4360953814 hasConcept C59822182 @default.
- W4360953814 hasConcept C86803240 @default.
- W4360953814 hasConceptScore W4360953814C104317684 @default.
- W4360953814 hasConceptScore W4360953814C104397665 @default.
- W4360953814 hasConceptScore W4360953814C150194340 @default.
- W4360953814 hasConceptScore W4360953814C2781255879 @default.
- W4360953814 hasConceptScore W4360953814C2781429666 @default.
- W4360953814 hasConceptScore W4360953814C54355233 @default.
- W4360953814 hasConceptScore W4360953814C59822182 @default.
- W4360953814 hasConceptScore W4360953814C86803240 @default.
- W4360953814 hasIssue "01" @default.
- W4360953814 hasLocation W43609538141 @default.
- W4360953814 hasOpenAccess W4360953814 @default.
- W4360953814 hasPrimaryLocation W43609538141 @default.
- W4360953814 hasRelatedWork W1991997168 @default.
- W4360953814 hasRelatedWork W2001677368 @default.
- W4360953814 hasRelatedWork W2002128513 @default.
- W4360953814 hasRelatedWork W2009966535 @default.
- W4360953814 hasRelatedWork W2277438425 @default.
- W4360953814 hasRelatedWork W2985738900 @default.
- W4360953814 hasRelatedWork W3173277914 @default.
- W4360953814 hasRelatedWork W4293746088 @default.
- W4360953814 hasRelatedWork W4360953814 @default.
- W4360953814 hasRelatedWork W2092874662 @default.
- W4360953814 hasVolume "25" @default.
- W4360953814 isParatext "false" @default.
- W4360953814 isRetracted "false" @default.
- W4360953814 workType "article" @default.