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• W4360982634 abstract "Abstract To facilitate our understanding of the often rapid and nuanced dynamics of extracellularly exposed proteomes during signaling events, it is important to devise robust workflows affording fast time resolution without biases and confounding factors. Here, we present S urface-exposed protein La beling using P eroxida S e, H 2 O 2 , and T yramide-derivative (SLAPSHOT), to label extracellularly exposed proteins in a rapid, sensitive, and specific manner, while preserving cellular integrity. This experimentally simple and flexible method utilizes recombinant soluble APEX2 peroxidase that is applied to cells, thus circumventing biological perturbations, tedious engineering of tools and cells, and labeling biases. APEX2 neither requires metal cations for activity nor contains disulfide bonds, conferring versatility for a wide spectrum of experimental setups. We applied SLAPSHOT followed by quantitative mass spectrometry-based proteomics analysis to examine the immediate and extensive cell surface expansion and ensuing restorative membrane shedding upon the activation of Scott syndrome-linked TMEM16F, a ubiquitously expressed calcium-dependent phospholipid scramblase and ion channel. Time-course data ranging from one to thirty minutes of calcium stimulation using wild-type and TMEM16F deficient cells revealed intricate co-regulation of known protein families, including those in the integrin and ICAM families. Crucially, we identified proteins that are known to reside in intracellular organelles, including ER, as occupants of the freshly deposited membrane, and mitovesicles as an abundant component and contributor to the extracellularly exposed proteome. Our study not only provides the first accounts of the immediate consequences of calcium signaling on the extracellularly exposed proteome, but also presents a blueprint for the application of SLAPSHOT as a general approach for monitoring extracellularly exposed protein dynamics. Highlights An enzyme-driven method to tag extracellularly exposed proteins in an unbiased manner with a superior combination of temporal resolution, spatial specificity, and sensitivity A general approach applicable to primary and scarce cells without involving cellular engineering Short time scale proteome dynamics of Jurkat cells with and without TMEM16F revealed by SLAPSHOT coupled with quantitative mass spectrometry provide insights into phospholipid scrambling-mediated plasma membrane remodeling" @default.
• W4360982634 created "2023-03-30" @default.
• W4360982634 creator A5002084451 @default.
• W4360982634 creator A5041305380 @default.
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• W4360982634 creator A5082002650 @default.
• W4360982634 date "2023-03-26" @default.
• W4360982634 modified "2023-09-23" @default.
• W4360982634 title "SLAPSHOT reveals rapid dynamics of extracellularly exposed proteome in response to calcium-activated plasma membrane phospholipid scrambling" @default.
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• W4360982634 cites W1569789584 @default.
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• W4360982634 cites W1982458319 @default.
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• W4360982634 cites W2115942040 @default.
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• W4360982634 cites W2157918066 @default.
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• W4360982634 cites W2159675211 @default.
• W4360982634 cites W2167279371 @default.
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• W4360982634 doi "https://doi.org/10.1101/2023.03.26.534250" @default.
• W4360982634 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36993417" @default.
• W4360982634 hasPublicationYear "2023" @default.
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