Matches in SemOpenAlex for { <https://semopenalex.org/work/W4360992859> ?p ?o ?g. }
- W4360992859 abstract "Antibodies bind target molecules with exquisite specificity. The removal of these targets is mediated by the effector functions of antibodies. We reported earlier that the monoclonal antibody (mAb) 3F6 promotes opsonophagocytic killing of Staphylococcus aureus in blood and reduces bacterial replication in animals. Here, we generated mouse immunoglobulin G (mIgG) subclass variants and observed a hierarchy in protective efficacy 3F6-mIgG2a > 3F6-mIgG1 ≥ 3F6-mIgG2b >> 3F6-mIgG3 following bloodstream challenge of C57BL/6J mice. This hierarchy was not observed in BALB/cJ mice: All IgG subclasses conferred similar protection. IgG subclasses differ in their ability to activate complement and interact with Fcγ receptors (FcγR) on immune cells. 3F6-mIgG2a-dependent protection was lost in FcγR-deficient, but not in complement-deficient C57BL/6J animals. Measurements of the relative ratio of FcγRIV over complement receptor 3 (CR3) on neutrophils suggest the preferential expression of FcγRIV in C57BL/6 mice and of CR3 in BALB/cJ mice. To determine the physiological significance of these differing ratios, blocking antibodies against FcγRIV or CR3 were administered to animals before challenge. Correlating with the relative abundance of each receptor, 3F6-mIgG2a-dependent protection in C57BL/6J mice showed a greater reliance for FcγRIV while protection in BALB/cJ mice was only impaired upon neutralization of CR3. Thus, 3F6-based clearance of S. aureus in mice relies on a strain-specific contribution of variable FcγR- and complement-dependent pathways. We surmise that these variabilities are the result of genetic polymorphism(s) that may be encountered in other mammals including humans and may have clinical implications in predicting the efficacy of mAb-based therapies." @default.
- W4360992859 created "2023-03-30" @default.
- W4360992859 creator A5005368537 @default.
- W4360992859 creator A5010964030 @default.
- W4360992859 creator A5025345631 @default.
- W4360992859 creator A5036627086 @default.
- W4360992859 creator A5040442255 @default.
- W4360992859 creator A5041967827 @default.
- W4360992859 creator A5056056127 @default.
- W4360992859 creator A5083683183 @default.
- W4360992859 date "2023-03-27" @default.
- W4360992859 modified "2023-09-28" @default.
- W4360992859 title "Immunoglobulin G subclasses confer protection against <i>Staphylococcus aureus</i> bloodstream dissemination through distinct mechanisms in mouse models" @default.
- W4360992859 cites W1695644463 @default.
- W4360992859 cites W1716237860 @default.
- W4360992859 cites W1878404532 @default.
- W4360992859 cites W1944603902 @default.
- W4360992859 cites W1962890795 @default.
- W4360992859 cites W1963492926 @default.
- W4360992859 cites W1964038763 @default.
- W4360992859 cites W1967113354 @default.
- W4360992859 cites W1990621187 @default.
- W4360992859 cites W1991656907 @default.
- W4360992859 cites W1995731982 @default.
- W4360992859 cites W2016708646 @default.
- W4360992859 cites W2024683205 @default.
- W4360992859 cites W2037311267 @default.
- W4360992859 cites W2048787993 @default.
- W4360992859 cites W2057745345 @default.
- W4360992859 cites W2064648061 @default.
- W4360992859 cites W2067217824 @default.
- W4360992859 cites W2069699883 @default.
- W4360992859 cites W2071843728 @default.
- W4360992859 cites W2072577743 @default.
- W4360992859 cites W2075119181 @default.
- W4360992859 cites W2086542713 @default.
- W4360992859 cites W2090977381 @default.
- W4360992859 cites W2094578161 @default.
- W4360992859 cites W2100783252 @default.
- W4360992859 cites W2104823480 @default.
- W4360992859 cites W2106279568 @default.
- W4360992859 cites W2107321529 @default.
- W4360992859 cites W2110990893 @default.
- W4360992859 cites W2114914493 @default.
- W4360992859 cites W2116037635 @default.
- W4360992859 cites W2116607488 @default.
- W4360992859 cites W2125066910 @default.
- W4360992859 cites W2128358667 @default.
- W4360992859 cites W2130762696 @default.
- W4360992859 cites W2130837689 @default.
- W4360992859 cites W2136123895 @default.
- W4360992859 cites W2136648677 @default.
- W4360992859 cites W2142456569 @default.
- W4360992859 cites W2148404819 @default.
- W4360992859 cites W2148906904 @default.
- W4360992859 cites W2152783224 @default.
- W4360992859 cites W2161096815 @default.
- W4360992859 cites W2168284316 @default.
- W4360992859 cites W2169856449 @default.
- W4360992859 cites W2239738725 @default.
- W4360992859 cites W2291901757 @default.
- W4360992859 cites W2314649741 @default.
- W4360992859 cites W2345602998 @default.
- W4360992859 cites W2380566153 @default.
- W4360992859 cites W2427795348 @default.
- W4360992859 cites W2479623580 @default.
- W4360992859 cites W2510720247 @default.
- W4360992859 cites W2518557131 @default.
- W4360992859 cites W2535995444 @default.
- W4360992859 cites W2739115819 @default.
- W4360992859 cites W2774160955 @default.
- W4360992859 cites W2894305622 @default.
- W4360992859 cites W2904377140 @default.
- W4360992859 cites W2941324075 @default.
- W4360992859 cites W2974014568 @default.
- W4360992859 cites W2985801624 @default.
- W4360992859 cites W3005303620 @default.
- W4360992859 cites W3014871233 @default.
- W4360992859 cites W3081330967 @default.
- W4360992859 cites W3084662876 @default.
- W4360992859 cites W3127046227 @default.
- W4360992859 cites W3155208265 @default.
- W4360992859 cites W3159112722 @default.
- W4360992859 cites W3165080900 @default.
- W4360992859 cites W31738964 @default.
- W4360992859 cites W4205881333 @default.
- W4360992859 cites W4280585015 @default.
- W4360992859 cites W4284898818 @default.
- W4360992859 cites W4293102349 @default.
- W4360992859 cites W4313377124 @default.
- W4360992859 doi "https://doi.org/10.1073/pnas.2220765120" @default.
- W4360992859 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36972444" @default.
- W4360992859 hasPublicationYear "2023" @default.
- W4360992859 type Work @default.
- W4360992859 citedByCount "0" @default.
- W4360992859 crossrefType "journal-article" @default.
- W4360992859 hasAuthorship W4360992859A5005368537 @default.
- W4360992859 hasAuthorship W4360992859A5010964030 @default.
- W4360992859 hasAuthorship W4360992859A5025345631 @default.
- W4360992859 hasAuthorship W4360992859A5036627086 @default.