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- W4361002034 abstract "Here we present a modified version of : dx.doi.org/10.17504/protocols.io.261genwm7g47/v2 that was used to successfully extract nuclei from an array of different tissue types for single cell sequencing and modify it with the purpose of extracting nuclei for single cell multiome ATAC and gene expression on the 10x chromium. The modifications for this protocol include: different concentration of RNase inhibitors, different quantities for nuclear isolation buffer, removal of unnecessary steps as well as QC specific for multiome analysis. If you are looking to use this protocol for bulk ATAC-seq use of protease inhibitor cocktail PIC is recommended instead of RNase inhibitor on the snRNA-seq version of this protocol (dx.doi.org/10.17504/protocols.io.261genwm7g47/v2) please get in touch with the authors if you are unsure on how to do this." @default.
- W4361002034 created "2023-03-30" @default.
- W4361002034 creator A5001642467 @default.
- W4361002034 date "2023-03-06" @default.
- W4361002034 modified "2023-09-25" @default.
- W4361002034 title "A versatile nuclei extraction protocol for single cell multiome ATAC and gene expression in non model species v2" @default.
- W4361002034 doi "https://doi.org/10.17504/protocols.io.261genwm7g47/v3" @default.
- W4361002034 hasPublicationYear "2023" @default.
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