Matches in SemOpenAlex for { <https://semopenalex.org/work/W4361002037> ?p ?o ?g. }
- W4361002037 endingPage "594.e5" @default.
- W4361002037 startingPage "584" @default.
- W4361002037 abstract "The Notch signaling pathway, an important cell fate determination pathway, is modulated by the ubiquitin ligase Deltex. Here, we investigate the structural basis for Deltex-Notch interaction. We used nuclear magnetic resonance (NMR) spectroscopy to assign the backbone of the Drosophila Deltex WWE2 domain and mapped the binding site of the Notch ankyrin (ANK) domain to the N-terminal WWEA motif. Using cultured Drosophila S2R+ cells, we find that point substitutions within the ANK-binding surface of Deltex disrupt Deltex-mediated enhancement of Notch transcriptional activation and disrupt ANK binding in cells and in vitro. Likewise, ANK substitutions that disrupt Notch-Deltex heterodimer formation in vitro block disrupt Deltex-mediated stimulation of Notch transcription activation and diminish interaction with full-length Deltex in cells. Surprisingly, the Deltex-Notch intracellular domain (NICD) interaction is not disrupted by deletion of the Deltex WWE2 domain, suggesting a secondary Notch-Deltex interaction. These results show the importance of the WWEA:ANK interaction in enhancing Notch signaling." @default.
- W4361002037 created "2023-03-30" @default.
- W4361002037 creator A5019100333 @default.
- W4361002037 creator A5036959751 @default.
- W4361002037 creator A5072361183 @default.
- W4361002037 creator A5079195698 @default.
- W4361002037 creator A5082077539 @default.
- W4361002037 creator A5083396495 @default.
- W4361002037 date "2023-05-01" @default.
- W4361002037 modified "2023-10-14" @default.
- W4361002037 title "Structural features of the Notch ankyrin domain-Deltex WWE2 domain heterodimer determined by NMR spectroscopy and functional implications" @default.
- W4361002037 cites W1572790640 @default.
- W4361002037 cites W1597435584 @default.
- W4361002037 cites W1795830070 @default.
- W4361002037 cites W1824693654 @default.
- W4361002037 cites W1955892023 @default.
- W4361002037 cites W1963828768 @default.
- W4361002037 cites W1972168412 @default.
- W4361002037 cites W1977661665 @default.
- W4361002037 cites W1980788640 @default.
- W4361002037 cites W1997772586 @default.
- W4361002037 cites W2013732926 @default.
- W4361002037 cites W2025983897 @default.
- W4361002037 cites W2036540072 @default.
- W4361002037 cites W2037684193 @default.
- W4361002037 cites W2039109621 @default.
- W4361002037 cites W2043896520 @default.
- W4361002037 cites W2045165927 @default.
- W4361002037 cites W2047525285 @default.
- W4361002037 cites W2050662405 @default.
- W4361002037 cites W2051603362 @default.
- W4361002037 cites W2060619683 @default.
- W4361002037 cites W2061645385 @default.
- W4361002037 cites W2084520007 @default.
- W4361002037 cites W2086059239 @default.
- W4361002037 cites W2089640234 @default.
- W4361002037 cites W2103723119 @default.
- W4361002037 cites W2111341494 @default.
- W4361002037 cites W2125757476 @default.
- W4361002037 cites W2127839852 @default.
- W4361002037 cites W2131004933 @default.
- W4361002037 cites W2140239718 @default.
- W4361002037 cites W2142757818 @default.
- W4361002037 cites W2144599076 @default.
- W4361002037 cites W2147298899 @default.
- W4361002037 cites W2149649384 @default.
- W4361002037 cites W2160362993 @default.
- W4361002037 cites W2166931568 @default.
- W4361002037 cites W2169821755 @default.
- W4361002037 cites W2376573086 @default.
- W4361002037 cites W2403998678 @default.
- W4361002037 cites W2419558864 @default.
- W4361002037 cites W2465000744 @default.
- W4361002037 cites W2468328155 @default.
- W4361002037 cites W2771937306 @default.
- W4361002037 cites W2883624239 @default.
- W4361002037 cites W2884007012 @default.
- W4361002037 cites W2927742965 @default.
- W4361002037 cites W3081212540 @default.
- W4361002037 cites W3183475563 @default.
- W4361002037 cites W4240748016 @default.
- W4361002037 doi "https://doi.org/10.1016/j.str.2023.03.003" @default.
- W4361002037 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36977409" @default.
- W4361002037 hasPublicationYear "2023" @default.
- W4361002037 type Work @default.
- W4361002037 citedByCount "1" @default.
- W4361002037 countsByYear W43610020372023 @default.
- W4361002037 crossrefType "journal-article" @default.
- W4361002037 hasAuthorship W4361002037A5019100333 @default.
- W4361002037 hasAuthorship W4361002037A5036959751 @default.
- W4361002037 hasAuthorship W4361002037A5072361183 @default.
- W4361002037 hasAuthorship W4361002037A5079195698 @default.
- W4361002037 hasAuthorship W4361002037A5082077539 @default.
- W4361002037 hasAuthorship W4361002037A5083396495 @default.
- W4361002037 hasConcept C104317684 @default.
- W4361002037 hasConcept C113241181 @default.
- W4361002037 hasConcept C161879069 @default.
- W4361002037 hasConcept C202769670 @default.
- W4361002037 hasConcept C24284526 @default.
- W4361002037 hasConcept C5432534 @default.
- W4361002037 hasConcept C54355233 @default.
- W4361002037 hasConcept C62478195 @default.
- W4361002037 hasConcept C65114990 @default.
- W4361002037 hasConcept C86339819 @default.
- W4361002037 hasConcept C86803240 @default.
- W4361002037 hasConcept C95444343 @default.
- W4361002037 hasConceptScore W4361002037C104317684 @default.
- W4361002037 hasConceptScore W4361002037C113241181 @default.
- W4361002037 hasConceptScore W4361002037C161879069 @default.
- W4361002037 hasConceptScore W4361002037C202769670 @default.
- W4361002037 hasConceptScore W4361002037C24284526 @default.
- W4361002037 hasConceptScore W4361002037C5432534 @default.
- W4361002037 hasConceptScore W4361002037C54355233 @default.
- W4361002037 hasConceptScore W4361002037C62478195 @default.
- W4361002037 hasConceptScore W4361002037C65114990 @default.
- W4361002037 hasConceptScore W4361002037C86339819 @default.
- W4361002037 hasConceptScore W4361002037C86803240 @default.
- W4361002037 hasConceptScore W4361002037C95444343 @default.