Matches in SemOpenAlex for { <https://semopenalex.org/work/W4361010754> ?p ?o ?g. }
- W4361010754 endingPage "1010" @default.
- W4361010754 startingPage "1010" @default.
- W4361010754 abstract "Within the neurovascular unit, brain pericytes (BPs) are of major importance for the induction and maintenance of the properties of the blood-brain barrier (BBB) carried by the brain microvessel endothelial cells (ECs). Throughout barriergenesis, ECs take advantage of soluble elements or contact with BPs to maintain BBB integrity and the regulation of their cellular homeostasis. However, very few studies have focused on the role of ECs in the maturation of BPs. The aim of this study is to shed light on the proteome of BPs solocultured (hBP-solo) or cocultured with ECs (hBP-coc) to model the human BBB in a non-contact manner. We first generated protein libraries for each condition and identified 2233 proteins in hBP-solo versus 2492 in hBP-coc and 2035 common proteins. We performed a quantification of the enriched proteins in each condition by sequential window acquisition of all theoretical mass spectra (SWATH) analysis. We found 51 proteins enriched in hBP-solo related to cell proliferation, contractility, adhesion and extracellular matrix element production, a protein pattern related to an immature cell. In contrast, 90 proteins are enriched in hBP-coc associated with a reduction in contractile activities as observed in vivo in 'mature' BPs, and a significant gain in different metabolic functions, particularly related to mitochondrial activities and sterol metabolism. This study highlights that BPs take advantage of ECs during barriergenesis to make a metabolic switch in favor of BBB homeostasis in vitro." @default.
- W4361010754 created "2023-03-30" @default.
- W4361010754 creator A5001885300 @default.
- W4361010754 creator A5007821161 @default.
- W4361010754 creator A5039254796 @default.
- W4361010754 creator A5046200375 @default.
- W4361010754 creator A5050089613 @default.
- W4361010754 creator A5052372115 @default.
- W4361010754 creator A5054545486 @default.
- W4361010754 creator A5063640316 @default.
- W4361010754 creator A5066298190 @default.
- W4361010754 creator A5073977756 @default.
- W4361010754 date "2023-03-25" @default.
- W4361010754 modified "2023-10-18" @default.
- W4361010754 title "Highlighting In Vitro the Role of Brain-like Endothelial Cells on the Maturation and Metabolism of Brain Pericytes by SWATH Proteomics" @default.
- W4361010754 cites W1260393137 @default.
- W4361010754 cites W1522962880 @default.
- W4361010754 cites W1540025214 @default.
- W4361010754 cites W1559124240 @default.
- W4361010754 cites W1591858261 @default.
- W4361010754 cites W1605878984 @default.
- W4361010754 cites W1717020381 @default.
- W4361010754 cites W185386472 @default.
- W4361010754 cites W1969123951 @default.
- W4361010754 cites W1972529301 @default.
- W4361010754 cites W1975478041 @default.
- W4361010754 cites W1997912679 @default.
- W4361010754 cites W2004753542 @default.
- W4361010754 cites W2006322997 @default.
- W4361010754 cites W2008774890 @default.
- W4361010754 cites W2008869013 @default.
- W4361010754 cites W2009184205 @default.
- W4361010754 cites W2011874786 @default.
- W4361010754 cites W2019780808 @default.
- W4361010754 cites W2039999119 @default.
- W4361010754 cites W2042493192 @default.
- W4361010754 cites W2052578334 @default.
- W4361010754 cites W2056856515 @default.
- W4361010754 cites W2056863014 @default.
- W4361010754 cites W2059256286 @default.
- W4361010754 cites W2062035530 @default.
- W4361010754 cites W2067935721 @default.
- W4361010754 cites W2069929255 @default.
- W4361010754 cites W2071867975 @default.
- W4361010754 cites W2072033965 @default.
- W4361010754 cites W2075175167 @default.
- W4361010754 cites W2076088544 @default.
- W4361010754 cites W2078959938 @default.
- W4361010754 cites W2079013149 @default.
- W4361010754 cites W2079843936 @default.
- W4361010754 cites W2088364769 @default.
- W4361010754 cites W2092671785 @default.
- W4361010754 cites W2107325549 @default.
- W4361010754 cites W2107798346 @default.
- W4361010754 cites W2109995938 @default.
- W4361010754 cites W2115390024 @default.
- W4361010754 cites W2115598567 @default.
- W4361010754 cites W2118555325 @default.
- W4361010754 cites W2119528478 @default.
- W4361010754 cites W2121674642 @default.
- W4361010754 cites W2121885194 @default.
- W4361010754 cites W2128331079 @default.
- W4361010754 cites W2145392559 @default.
- W4361010754 cites W2146544786 @default.
- W4361010754 cites W2153787061 @default.
- W4361010754 cites W2158250531 @default.
- W4361010754 cites W2159675211 @default.
- W4361010754 cites W2169199162 @default.
- W4361010754 cites W2171632655 @default.
- W4361010754 cites W2476542314 @default.
- W4361010754 cites W2510962431 @default.
- W4361010754 cites W2620436731 @default.
- W4361010754 cites W2773325366 @default.
- W4361010754 cites W2786514708 @default.
- W4361010754 cites W2788184839 @default.
- W4361010754 cites W2890224686 @default.
- W4361010754 cites W2899585717 @default.
- W4361010754 cites W2904598213 @default.
- W4361010754 cites W2928665623 @default.
- W4361010754 cites W2936948584 @default.
- W4361010754 cites W2949897197 @default.
- W4361010754 cites W2950764390 @default.
- W4361010754 cites W2966919529 @default.
- W4361010754 cites W2972610703 @default.
- W4361010754 cites W3014418260 @default.
- W4361010754 cites W3022539327 @default.
- W4361010754 cites W3028708621 @default.
- W4361010754 cites W3041881289 @default.
- W4361010754 cites W3045585426 @default.
- W4361010754 cites W3047045591 @default.
- W4361010754 cites W3047828847 @default.
- W4361010754 cites W3107527779 @default.
- W4361010754 cites W3111273864 @default.
- W4361010754 cites W3118436183 @default.
- W4361010754 cites W3130987271 @default.
- W4361010754 cites W3135685389 @default.
- W4361010754 cites W3138224055 @default.
- W4361010754 cites W3154406044 @default.