Matches in SemOpenAlex for { <https://semopenalex.org/work/W4361019199> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W4361019199 endingPage "679" @default.
- W4361019199 startingPage "669" @default.
- W4361019199 abstract "Colistin is a last resort drug for the treatment of multiple drug-resistant (MDR) Gram-negative bacterial infections. Rapid methods to detect resistance are highly desirable. Here, we evaluated the performance of a commercially available MALDI-TOF MS-based assay for colistin resistance testing in Escherichia coli at two different sites. Ninety clinical E. coli isolates were provided by France and tested in Germany and UK using a MALDI-TOF MS-based colistin resistance assay. Lipid A molecules of the bacterial cell membrane were extracted using the MBT Lipid Xtract Kit™ (RUO; Bruker Daltonics, Germany). Spectra acquisition and evaluation were performed by the MBT HT LipidART Module of MBT Compass HT (RUO; Bruker Daltonics) on a MALDI Biotyper® sirius system (Bruker Daltonics) in negative ion mode. Phenotypic colistin resistance was determined by broth microdilution (MICRONAUT MIC-Strip Colistin, Bruker Daltonics) and used as a reference. Comparing the results of the MALDI-TOF MS-based colistin resistance assay with the data of the phenotypic reference method for the UK, sensitivity and specificity for the detection of colistin resistance were 97.1% (33/34) and 96.4% (53/55), respectively. Germany showed 97.1% (33/34) sensitivity and 100% (55/55) specificity for the detection of colistin resistance by MALDI-TOF MS. Applying the MBT Lipid Xtract™ Kit in combination with MALDI-TOF MS and dedicated software showed excellent performances for E. coli. Analytical and clinical validation studies must be performed to demonstrate the performance of the method as a diagnostic tool." @default.
- W4361019199 created "2023-03-30" @default.
- W4361019199 creator A5004982892 @default.
- W4361019199 creator A5025141902 @default.
- W4361019199 creator A5035086338 @default.
- W4361019199 creator A5035549078 @default.
- W4361019199 creator A5043370339 @default.
- W4361019199 creator A5048848554 @default.
- W4361019199 creator A5063752475 @default.
- W4361019199 date "2023-03-27" @default.
- W4361019199 modified "2023-10-16" @default.
- W4361019199 title "Two-site study on performances of a commercially available MALDI-TOF MS-based assay for the detection of colistin resistance in Escherichia coli" @default.
- W4361019199 cites W1606853044 @default.
- W4361019199 cites W2009562400 @default.
- W4361019199 cites W2116245384 @default.
- W4361019199 cites W2117795567 @default.
- W4361019199 cites W2179036997 @default.
- W4361019199 cites W2583196822 @default.
- W4361019199 cites W2593444269 @default.
- W4361019199 cites W2773164014 @default.
- W4361019199 cites W2801149479 @default.
- W4361019199 cites W2803249533 @default.
- W4361019199 cites W2810427827 @default.
- W4361019199 cites W2888805117 @default.
- W4361019199 cites W2899512978 @default.
- W4361019199 cites W2899549732 @default.
- W4361019199 cites W2913118041 @default.
- W4361019199 cites W2944063377 @default.
- W4361019199 cites W2979283057 @default.
- W4361019199 cites W2979566712 @default.
- W4361019199 cites W3015437338 @default.
- W4361019199 cites W3033171625 @default.
- W4361019199 cites W3133993931 @default.
- W4361019199 cites W3197044448 @default.
- W4361019199 cites W3199082916 @default.
- W4361019199 cites W4225485337 @default.
- W4361019199 doi "https://doi.org/10.1007/s10096-023-04587-9" @default.
- W4361019199 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36973378" @default.
- W4361019199 hasPublicationYear "2023" @default.
- W4361019199 type Work @default.
- W4361019199 citedByCount "0" @default.
- W4361019199 crossrefType "journal-article" @default.
- W4361019199 hasAuthorship W4361019199A5004982892 @default.
- W4361019199 hasAuthorship W4361019199A5025141902 @default.
- W4361019199 hasAuthorship W4361019199A5035086338 @default.
- W4361019199 hasAuthorship W4361019199A5035549078 @default.
- W4361019199 hasAuthorship W4361019199A5043370339 @default.
- W4361019199 hasAuthorship W4361019199A5048848554 @default.
- W4361019199 hasAuthorship W4361019199A5063752475 @default.
- W4361019199 hasBestOaLocation W43610191991 @default.
- W4361019199 hasConcept C104317684 @default.
- W4361019199 hasConcept C114851261 @default.
- W4361019199 hasConcept C176947019 @default.
- W4361019199 hasConcept C2779259085 @default.
- W4361019199 hasConcept C2780950330 @default.
- W4361019199 hasConcept C501593827 @default.
- W4361019199 hasConcept C547475151 @default.
- W4361019199 hasConcept C55493867 @default.
- W4361019199 hasConcept C71924100 @default.
- W4361019199 hasConcept C86803240 @default.
- W4361019199 hasConcept C89423630 @default.
- W4361019199 hasConceptScore W4361019199C104317684 @default.
- W4361019199 hasConceptScore W4361019199C114851261 @default.
- W4361019199 hasConceptScore W4361019199C176947019 @default.
- W4361019199 hasConceptScore W4361019199C2779259085 @default.
- W4361019199 hasConceptScore W4361019199C2780950330 @default.
- W4361019199 hasConceptScore W4361019199C501593827 @default.
- W4361019199 hasConceptScore W4361019199C547475151 @default.
- W4361019199 hasConceptScore W4361019199C55493867 @default.
- W4361019199 hasConceptScore W4361019199C71924100 @default.
- W4361019199 hasConceptScore W4361019199C86803240 @default.
- W4361019199 hasConceptScore W4361019199C89423630 @default.
- W4361019199 hasFunder F4320311904 @default.
- W4361019199 hasIssue "6" @default.
- W4361019199 hasLocation W43610191991 @default.
- W4361019199 hasLocation W43610191992 @default.
- W4361019199 hasOpenAccess W4361019199 @default.
- W4361019199 hasPrimaryLocation W43610191991 @default.
- W4361019199 hasRelatedWork W2123498714 @default.
- W4361019199 hasRelatedWork W2154635407 @default.
- W4361019199 hasRelatedWork W2364799643 @default.
- W4361019199 hasRelatedWork W2433524905 @default.
- W4361019199 hasRelatedWork W2555135577 @default.
- W4361019199 hasRelatedWork W2744408422 @default.
- W4361019199 hasRelatedWork W2885382822 @default.
- W4361019199 hasRelatedWork W3026441217 @default.
- W4361019199 hasRelatedWork W3115403981 @default.
- W4361019199 hasRelatedWork W4205760826 @default.
- W4361019199 hasVolume "42" @default.
- W4361019199 isParatext "false" @default.
- W4361019199 isRetracted "false" @default.
- W4361019199 workType "article" @default.