FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4361019781> ?p ?o ?g. }

• W4361019781 endingPage "e0010577" @default.
• W4361019781 startingPage "e0010577" @default.
• W4361019781 abstract "Schistosomiasis is a neglected water-born parasitic disease caused by Schistosoma affecting more than 200 million people. Introgressive hybridization is common among these parasites and raises issues concerning their zoonotic transmission. Morphological identification of Schistosoma cercariae is difficult and does not permit hybrids detection. Our objective was to assess the performance of MALDI-TOF (Matrix Assistated Laser Desorption-Ionization–Time Of Flight) mass spectrometry for the specific identification of cercariae in human and non-human Schistosoma and for the detection of hybridization between S . bovis and S . haematobium . Spectra were collected from laboratory reared molluscs infested with strains of S . haematobium , S . mansoni , S. bovis , S . rodhaini and S . bovis x S . haematobium natural (Corsican hybrid) and artificial hybrids. Cluster analysis showed a clear separation between S . haematobium , S . bovis , S . mansoni and S . rodhaini . Corsican hybrids are classified with those of the parental strain of S . haematobium whereas other hybrids formed a distinct cluster. In blind test analysis the developed MALDI-TOF spectral database permits identification of Schistosoma cercariae with high accuracy (94%) and good specificity ( S . bovis : 99.59%, S . haematobium 99.56%, S . mansoni and S . rodhaini : 100%). Most misidentifications were between S . haematobium and the Corsican hybrids. The use of machine learning permits to improve the discrimination between these last two taxa, with accuracy, F1 score and Sensitivity/Specificity > 97%. In multivariate analysis the factors associated with obtaining a valid identification score (> 1.7) were absence of ethanol preservation (p < 0.001) and a number of 2–3 cercariae deposited per well (p < 0.001). Also, spectra acquired from S . mansoni cercariae are more likely to obtain a valid identification score than those acquired from S . haematobium (p<0.001). MALDI-TOF is a reliable technique for high-throughput identification of Schistosoma cercariae of medical and veterinary importance and could be useful for field survey in endemic areas." @default.
• W4361019781 created "2023-03-30" @default.
• W4361019781 creator A5000530226 @default.
• W4361019781 creator A5003314744 @default.
• W4361019781 creator A5039185549 @default.
• W4361019781 creator A5047146958 @default.
• W4361019781 creator A5070130683 @default.
• W4361019781 date "2023-03-28" @default.
• W4361019781 modified "2023-10-11" @default.
• W4361019781 title "MALDI-TOF: A new tool for the identification of Schistosoma cercariae and detection of hybrids" @default.
• W4361019781 cites W1995789208 @default.
• W4361019781 cites W2006617902 @default.
• W4361019781 cites W2029903395 @default.
• W4361019781 cites W2033294362 @default.
• W4361019781 cites W2037046164 @default.
• W4361019781 cites W2037943927 @default.
• W4361019781 cites W2049095093 @default.
• W4361019781 cites W2098082628 @default.
• W4361019781 cites W2104318435 @default.
• W4361019781 cites W2107506178 @default.
• W4361019781 cites W2132753699 @default.
• W4361019781 cites W2136210388 @default.
• W4361019781 cites W2147214095 @default.
• W4361019781 cites W2162821280 @default.
• W4361019781 cites W2183691180 @default.
• W4361019781 cites W2216929255 @default.
• W4361019781 cites W2338755098 @default.
• W4361019781 cites W2470539112 @default.
• W4361019781 cites W2503655211 @default.
• W4361019781 cites W2504520985 @default.
• W4361019781 cites W2515887488 @default.
• W4361019781 cites W2556285179 @default.
• W4361019781 cites W2591340300 @default.
• W4361019781 cites W2738140991 @default.
• W4361019781 cites W2740705662 @default.
• W4361019781 cites W2762177954 @default.
• W4361019781 cites W2904167747 @default.
• W4361019781 cites W2921890953 @default.
• W4361019781 cites W2936174077 @default.
• W4361019781 cites W2947964313 @default.
• W4361019781 cites W2948501362 @default.
• W4361019781 cites W2984675404 @default.
• W4361019781 cites W3031793889 @default.
• W4361019781 cites W3048815844 @default.
• W4361019781 cites W3126857624 @default.
• W4361019781 cites W3135599194 @default.
• W4361019781 cites W3137528588 @default.
• W4361019781 cites W3138013683 @default.
• W4361019781 cites W3158917864 @default.
• W4361019781 cites W3184860359 @default.
• W4361019781 cites W3188285215 @default.
• W4361019781 cites W3195567711 @default.
• W4361019781 cites W3201317651 @default.
• W4361019781 cites W3201527060 @default.
• W4361019781 cites W4200008766 @default.
• W4361019781 cites W4200146279 @default.
• W4361019781 cites W4211093028 @default.
• W4361019781 cites W4211128418 @default.
• W4361019781 cites W4220874275 @default.
• W4361019781 cites W4220929417 @default.
• W4361019781 cites W208422122 @default.
• W4361019781 doi "https://doi.org/10.1371/journal.pntd.0010577" @default.
• W4361019781 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36976804" @default.
• W4361019781 hasPublicationYear "2023" @default.
• W4361019781 type Work @default.
• W4361019781 citedByCount "1" @default.
• W4361019781 crossrefType "journal-article" @default.
• W4361019781 hasAuthorship W4361019781A5000530226 @default.
• W4361019781 hasAuthorship W4361019781A5003314744 @default.
• W4361019781 hasAuthorship W4361019781A5039185549 @default.
• W4361019781 hasAuthorship W4361019781A5047146958 @default.
• W4361019781 hasAuthorship W4361019781A5070130683 @default.
• W4361019781 hasBestOaLocation W43610197811 @default.
• W4361019781 hasConcept C165901193 @default.
• W4361019781 hasConcept C2776225656 @default.
• W4361019781 hasConcept C2776863245 @default.
• W4361019781 hasConcept C2777445151 @default.
• W4361019781 hasConcept C42972112 @default.
• W4361019781 hasConcept C48189365 @default.
• W4361019781 hasConcept C59822182 @default.
• W4361019781 hasConcept C71924100 @default.
• W4361019781 hasConcept C86803240 @default.
• W4361019781 hasConcept C90856448 @default.
• W4361019781 hasConceptScore W4361019781C165901193 @default.
• W4361019781 hasConceptScore W4361019781C2776225656 @default.
• W4361019781 hasConceptScore W4361019781C2776863245 @default.
• W4361019781 hasConceptScore W4361019781C2777445151 @default.
• W4361019781 hasConceptScore W4361019781C42972112 @default.
• W4361019781 hasConceptScore W4361019781C48189365 @default.
• W4361019781 hasConceptScore W4361019781C59822182 @default.
• W4361019781 hasConceptScore W4361019781C71924100 @default.
• W4361019781 hasConceptScore W4361019781C86803240 @default.
• W4361019781 hasConceptScore W4361019781C90856448 @default.
• W4361019781 hasFunder F4320320883 @default.
• W4361019781 hasIssue "3" @default.
• W4361019781 hasLocation W43610197811 @default.
• W4361019781 hasLocation W43610197812 @default.
• W4361019781 hasLocation W43610197813 @default.

• next