Matches in SemOpenAlex for { <https://semopenalex.org/work/W4361190816> ?p ?o ?g. }
- W4361190816 abstract "Transgenesis is an essential technique for any genetic model. Tol2-based transgenesis paired with Gateway-compatible vector collections has transformed zebrafish transgenesis with an accessible modular system. Here, we establish several next-generation transgenesis tools for zebrafish and other species to expand and enhance transgenic applications. To facilitate gene regulatory element testing, we generated Gateway middle entry vectors harboring the small mouse beta-globin minimal promoter coupled to several fluorophores, CreERT2 and Gal4. To extend the color spectrum for transgenic applications, we established middle entry vectors encoding the bright, blue-fluorescent protein mCerulean and mApple as an alternative red fluorophore. We present a series of p2A peptide-based 3' vectors with different fluorophores and subcellular localizations to co-label cells expressing proteins of interest. Finally, we established Tol2 destination vectors carrying the zebrafish exorh promoter driving different fluorophores as a pineal gland-specific transgenesis marker that is active before hatching and through adulthood. exorh-based reporters and transgenesis markers also drive specific pineal gland expression in the eye-less cavefish (Astyanax). Together, our vectors provide versatile reagents for transgenesis applications in zebrafish, cavefish and other models." @default.
- W4361190816 created "2023-03-31" @default.
- W4361190816 creator A5000670752 @default.
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- W4361190816 creator A5083078848 @default.
- W4361190816 date "2023-04-15" @default.
- W4361190816 modified "2023-10-15" @default.
- W4361190816 title "Next-generation plasmids for transgenesis in zebrafish and beyond" @default.
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