FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4361264871> ?p ?o ?g. }

• W4361264871 endingPage "105414" @default.
• W4361264871 startingPage "105414" @default.
• W4361264871 abstract "The γ-aminobutyric acid receptors (GABARs) mediate fast inhibitory transmission in central nervous system of insects and are important targets of insecticides. An auxiliary subunit, Shisa7, was identified in mammals as a single-passing transmembrane protein. However, the homology gene(s) of Shisa in invertebrates has not been reported to date. In the present study, a homolog Shisa gene was identified from the two-spotted spider mite, Tetranychus urticae Koch. Its open reading frame had 927 base pairs and encoded 308 amino acid residues, which has a typical Shisa domain at 13th-181st amino acid residues. According to the phylogenetic tree, the invertebrate Shisa was categorized apart with those of vertebrate, and TuShisa showed closest relationship with the Shisa9 of velvet mite, Dinothrombium tinctorium (L.). In the electrophysiological assay with two-electrode voltage clamp, the GABA-activated TuRDL channel was functionally formed in the Africa clawed frog Xenopus laevis (Daudin) oocytes (EC50 = 53.34 μM). No GABA-activated current could be observed in TuShisa-expressed oocytes, whereas TuShisa could reduce the sensitivity of TuRDL/TuShisa (mass ratio of 1: 4) channel to GABA. The homology structural models of TuRDL and TuShisa were built by the SWISS-MODEL server, their interaction was predicted using Z-DOCK and three predicted hydrogen bonds and interface residues were analysed by PyMOL. Meanwhile, the key interface residues of TuShisa affected the stability of complex were calculated by Discovery Studio 2019. In conclusion, the TuShisa, as the first reported invertebrate Shisa, was explored and functionally examined as the GABARs auxiliary subunit. Our findings provide a basis for research of invertebrate Shisa." @default.
• W4361264871 created "2023-03-31" @default.
• W4361264871 creator A5018863416 @default.
• W4361264871 creator A5030506495 @default.
• W4361264871 creator A5031275786 @default.
• W4361264871 creator A5035424826 @default.
• W4361264871 creator A5050339559 @default.
• W4361264871 creator A5067111030 @default.
• W4361264871 creator A5080119274 @default.
• W4361264871 creator A5089032744 @default.
• W4361264871 creator A5089585278 @default.
• W4361264871 date "2023-05-01" @default.
• W4361264871 modified "2023-09-27" @default.
• W4361264871 title "Shisa reduces the sensitivity of homomeric RDL channel to GABA in the two-spotted spider mite, Tetranychus urticae Koch" @default.
• W4361264871 cites W1089160263 @default.
• W4361264871 cites W1963561286 @default.
• W4361264871 cites W1969196466 @default.
• W4361264871 cites W1970774603 @default.
• W4361264871 cites W1986102335 @default.
• W4361264871 cites W1992192767 @default.
• W4361264871 cites W2002965963 @default.
• W4361264871 cites W2053072415 @default.
• W4361264871 cites W2070414942 @default.
• W4361264871 cites W2072885702 @default.
• W4361264871 cites W2078615673 @default.
• W4361264871 cites W2081602909 @default.
• W4361264871 cites W2144718863 @default.
• W4361264871 cites W2152207030 @default.
• W4361264871 cites W2152301430 @default.
• W4361264871 cites W2159614853 @default.
• W4361264871 cites W2168900836 @default.
• W4361264871 cites W2170564629 @default.
• W4361264871 cites W2176076159 @default.
• W4361264871 cites W2207857447 @default.
• W4361264871 cites W2283146683 @default.
• W4361264871 cites W2292475044 @default.
• W4361264871 cites W2293868972 @default.
• W4361264871 cites W2335748938 @default.
• W4361264871 cites W2346810207 @default.
• W4361264871 cites W2587638657 @default.
• W4361264871 cites W2592656674 @default.
• W4361264871 cites W2774477543 @default.
• W4361264871 cites W2794336788 @default.
• W4361264871 cites W2886894421 @default.
• W4361264871 cites W2923970954 @default.
• W4361264871 cites W2969726813 @default.
• W4361264871 cites W2980150274 @default.
• W4361264871 cites W2998471366 @default.
• W4361264871 cites W3093868212 @default.
• W4361264871 cites W3137922269 @default.
• W4361264871 cites W3156458251 @default.
• W4361264871 cites W3201057768 @default.
• W4361264871 cites W3203314034 @default.
• W4361264871 cites W3214531989 @default.
• W4361264871 cites W4205425254 @default.
• W4361264871 doi "https://doi.org/10.1016/j.pestbp.2023.105414" @default.
• W4361264871 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37105623" @default.
• W4361264871 hasPublicationYear "2023" @default.
• W4361264871 type Work @default.
• W4361264871 citedByCount "0" @default.
• W4361264871 crossrefType "journal-article" @default.
• W4361264871 hasAuthorship W4361264871A5018863416 @default.
• W4361264871 hasAuthorship W4361264871A5030506495 @default.
• W4361264871 hasAuthorship W4361264871A5031275786 @default.
• W4361264871 hasAuthorship W4361264871A5035424826 @default.
• W4361264871 hasAuthorship W4361264871A5050339559 @default.
• W4361264871 hasAuthorship W4361264871A5067111030 @default.
• W4361264871 hasAuthorship W4361264871A5080119274 @default.
• W4361264871 hasAuthorship W4361264871A5089032744 @default.
• W4361264871 hasAuthorship W4361264871A5089585278 @default.
• W4361264871 hasConcept C104292427 @default.
• W4361264871 hasConcept C104317684 @default.
• W4361264871 hasConcept C118892022 @default.
• W4361264871 hasConcept C15358309 @default.
• W4361264871 hasConcept C169627665 @default.
• W4361264871 hasConcept C170493617 @default.
• W4361264871 hasConcept C181199279 @default.
• W4361264871 hasConcept C2776104367 @default.
• W4361264871 hasConcept C2776906779 @default.
• W4361264871 hasConcept C2779248279 @default.
• W4361264871 hasConcept C2779535977 @default.
• W4361264871 hasConcept C2779711295 @default.
• W4361264871 hasConcept C515207424 @default.
• W4361264871 hasConcept C54355233 @default.
• W4361264871 hasConcept C55493867 @default.
• W4361264871 hasConcept C59822182 @default.
• W4361264871 hasConcept C61174792 @default.
• W4361264871 hasConcept C86803240 @default.
• W4361264871 hasConceptScore W4361264871C104292427 @default.
• W4361264871 hasConceptScore W4361264871C104317684 @default.
• W4361264871 hasConceptScore W4361264871C118892022 @default.
• W4361264871 hasConceptScore W4361264871C15358309 @default.
• W4361264871 hasConceptScore W4361264871C169627665 @default.
• W4361264871 hasConceptScore W4361264871C170493617 @default.
• W4361264871 hasConceptScore W4361264871C181199279 @default.
• W4361264871 hasConceptScore W4361264871C2776104367 @default.
• W4361264871 hasConceptScore W4361264871C2776906779 @default.
• W4361264871 hasConceptScore W4361264871C2779248279 @default.

• next