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• W4361269466 abstract "Gold nanoparticles (GNPs) have been used as detection probes for rapid and sensitive detection of various analytes, including bacteria. Here, we demonstrate a simple strategy for bacterial detection using GNPs functionalized with 4-mercaptophenylboronic acid (4-MPBA). 4-MPBA can interact with peptidoglycan or lipopolysaccharides present in bacterial organelles. After the addition of a high concentration of sodium hydroxide (NaOH), the functionalization of the surface of 50 nm GNPs with 4-MPBA (4-MPBA@GNPs) in the presence of polyethylene glycol results in a color change because of the aggregation of 4-MPBA@GNPs. This color change is dependent on the amount of bacteria present in the tested samples. Escherichia coli (E. coli) K-12 and Staphylococcus aureus (S. aureus) are used as Gram-negative and Gram-positive bacterial models, respectively. The color change can be detected within an hour by the naked eye. A linear relationship is observed between bacterial concentrations and the absorbance intensity at 533 nm; R2 values of 0.9152 and 0.8185 are obtained for E. coli K-12 and S. aureus, respectively. The limit of detection of E. coli K-12 is ∼2.38 × 102 CFU mL–1 and that of S. aureus is ∼4.77 × 103 CFU mL–1. This study provides a promising approach for the rapid detection of target Gram-negative and Gram-positive bacteria." @default.
• W4361269466 created "2023-03-31" @default.
• W4361269466 creator A5053348563 @default.
• W4361269466 creator A5057312438 @default.
• W4361269466 date "2023-03-30" @default.
• W4361269466 modified "2023-10-18" @default.
• W4361269466 title "Colorimetric Sensing of Gram-Negative and Gram-Positive Bacteria Using 4-Mercaptophenylboronic Acid-Functionalized Gold Nanoparticles in the Presence of Polyethylene Glycol" @default.
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• W4361269466 doi "https://doi.org/10.1021/acsomega.3c01205" @default.
• W4361269466 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37065017" @default.
• W4361269466 hasPublicationYear "2023" @default.
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