FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4361270099> ?p ?o ?g. }

• W4361270099 abstract "Abstract Objective : To use weighted gene co-expression network analysis (WGCNA) and molecular docking techniques to predict the mechanism of action of Huangqi Guizhi Wuwu decoction (HGWWD) in the treatment of osteoarthritis (OA) and to provide a bioinformatics basis for the clinical application of HGWWD in the treatment of OA. Methods : After searching the Gene Expression Omnibus (GEO) database, the GSE55235, GSE206848 and GSE55457 datasets were obtained. After merging and normalizing the GSE55235 and GSE206848 datasets, differentially expressed genes (DEGs) were screened, modules closely associated with OA were screened using a weighted gene co-expression network (WGCN) constructed using R software, and the common genes among DEGs and key module genes were imported into the Database for Annotation, Visualization and Integrated Discovery (DAVID) for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. The active ingredients in HGWWD were retrieved through the traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP), and a drug-active ingredient-target network was constructed using Cytoscape software. The GSE55457 dataset was used to verify and compare the expression differences in hub genes between different groups. AutoDock was used to conduct molecular docking of key genes and related active ingredients, and PyMOL and Discovery Studio were used for visual analyses. Results : A total of 1455 DEGs were screened in the merged dataset, including 574 upregulated genes and 881 downregulated genes. The key module contained 388 genes. A total of 47 pathways were obtained from the KEGG analysis. β-Sitosterol, kaempferol, β-carotene, stigmasterol, and quercetin were identified as the main compounds of HGWWD, and interleukin (IL)-6, prostaglandin-endoperoxide synthase 2 (PTGS2), vascular endothelial growth factor A (VEGFA), JUN, MYC, NFKBIA, SELE, and chemokine (C-X-C motif) ligand 2 (CXCL2) were the main hub genes involved in the treatment of OA with HGWWD. Molecular docking indicated that except for quercetin, the binding energies of other major compounds to hub genes were less than -5.0 kcal/mol. Conclusion : β-Sitosterol, kaempferol, β-carotene, stigmasterol and quercetin in HGWWD act on MYC, JUN, IL-6, VEGFA, and SELE, thus generating good therapeutic effects in individuals with OA." @default.
• W4361270099 created "2023-03-31" @default.
• W4361270099 creator A5014686910 @default.
• W4361270099 creator A5069639398 @default.
• W4361270099 creator A5074745812 @default.
• W4361270099 creator A5077687759 @default.
• W4361270099 date "2023-03-30" @default.
• W4361270099 modified "2023-10-17" @default.
• W4361270099 title "Mechanistic study of Huangqi Guizhi Wuwu decoction in the treatment of osteoarthritis using a weighted gene co-expression network and molecular docking techniques" @default.
• W4361270099 cites W2084694910 @default.
• W4361270099 cites W2749711897 @default.
• W4361270099 cites W2772070196 @default.
• W4361270099 cites W2895187627 @default.
• W4361270099 cites W2919475482 @default.
• W4361270099 cites W2941435286 @default.
• W4361270099 cites W2977897459 @default.
• W4361270099 cites W2995660260 @default.
• W4361270099 cites W3021543306 @default.
• W4361270099 cites W3120339302 @default.
• W4361270099 cites W3128653999 @default.
• W4361270099 cites W3133854280 @default.
• W4361270099 cites W3175953445 @default.
• W4361270099 cites W3184823685 @default.
• W4361270099 cites W4214613820 @default.
• W4361270099 cites W4283574965 @default.
• W4361270099 cites W4284879232 @default.
• W4361270099 cites W4288051170 @default.
• W4361270099 cites W4307778567 @default.
• W4361270099 cites W4315435959 @default.
• W4361270099 doi "https://doi.org/10.21203/rs.3.rs-2687352/v1" @default.
• W4361270099 hasPublicationYear "2023" @default.
• W4361270099 type Work @default.
• W4361270099 citedByCount "0" @default.
• W4361270099 crossrefType "posted-content" @default.
• W4361270099 hasAuthorship W4361270099A5014686910 @default.
• W4361270099 hasAuthorship W4361270099A5069639398 @default.
• W4361270099 hasAuthorship W4361270099A5074745812 @default.
• W4361270099 hasAuthorship W4361270099A5077687759 @default.
• W4361270099 hasBestOaLocation W43612700991 @default.
• W4361270099 hasConcept C104317684 @default.
• W4361270099 hasConcept C150194340 @default.
• W4361270099 hasConcept C152724338 @default.
• W4361270099 hasConcept C18431079 @default.
• W4361270099 hasConcept C2987395477 @default.
• W4361270099 hasConcept C54355233 @default.
• W4361270099 hasConcept C67339327 @default.
• W4361270099 hasConcept C70721500 @default.
• W4361270099 hasConcept C86803240 @default.
• W4361270099 hasConceptScore W4361270099C104317684 @default.
• W4361270099 hasConceptScore W4361270099C150194340 @default.
• W4361270099 hasConceptScore W4361270099C152724338 @default.
• W4361270099 hasConceptScore W4361270099C18431079 @default.
• W4361270099 hasConceptScore W4361270099C2987395477 @default.
• W4361270099 hasConceptScore W4361270099C54355233 @default.
• W4361270099 hasConceptScore W4361270099C67339327 @default.
• W4361270099 hasConceptScore W4361270099C70721500 @default.
• W4361270099 hasConceptScore W4361270099C86803240 @default.
• W4361270099 hasLocation W43612700991 @default.
• W4361270099 hasOpenAccess W4361270099 @default.
• W4361270099 hasPrimaryLocation W43612700991 @default.
• W4361270099 hasRelatedWork W2108901799 @default.
• W4361270099 hasRelatedWork W2754087939 @default.
• W4361270099 hasRelatedWork W2802217527 @default.
• W4361270099 hasRelatedWork W2909382709 @default.
• W4361270099 hasRelatedWork W2994628499 @default.
• W4361270099 hasRelatedWork W3135849929 @default.
• W4361270099 hasRelatedWork W3165029213 @default.
• W4361270099 hasRelatedWork W4281746354 @default.
• W4361270099 hasRelatedWork W4283810665 @default.
• W4361270099 hasRelatedWork W4360608455 @default.
• W4361270099 isParatext "false" @default.
• W4361270099 isRetracted "false" @default.
• W4361270099 workType "article" @default.