Matches in SemOpenAlex for { <https://semopenalex.org/work/W4361277650> ?p ?o ?g. }
- W4361277650 abstract "Abstract How enhancers regulate their target genes in the context of 3D chromatin organization is extensively studied and models which do not require direct enhancer-promoter contact have recently emerged. Here, we use the activation of estrogen receptor-dependent enhancers in a breast cancer cell line to study enhancer-promoter communication. This allows high temporal resolution tracking of molecular events from hormone stimulation to efficient gene activation. We examine how both enhancer-promoter spatial proximity assayed by DNA fluorescence in situ hybridization, and contact frequencies resulting from chromatin in situ fragmentation and proximity ligation by Capture-C, change dynamically during enhancer-driven gene activation. These orthogonal methods produce seemingly paradoxical results: upon enhancer activation enhancer-promoter contact frequencies increase while spatial proximity decreases. We explore this apparent discrepancy using different estrogen receptor ligands and transcription inhibitors. Our data demonstrate that enhancer-promoter contact frequencies are transcription independent but are influenced by enhancer-bound protein complexes whereas altered enhancer-promoter proximity depends on transcription. Our results emphasize that the relationship between contact frequencies and physical distance in the nucleus, especially over short genomic distances, is not always a simple one." @default.
- W4361277650 created "2023-03-31" @default.
- W4361277650 creator A5001379247 @default.
- W4361277650 creator A5016157467 @default.
- W4361277650 creator A5021382553 @default.
- W4361277650 creator A5035473961 @default.
- W4361277650 creator A5078926589 @default.
- W4361277650 date "2023-03-29" @default.
- W4361277650 modified "2023-10-03" @default.
- W4361277650 title "Transcription decouples estrogen-dependent changes in enhancer-promoter contact frequencies and spatial proximity" @default.
- W4361277650 cites W1826402150 @default.
- W4361277650 cites W1985621639 @default.
- W4361277650 cites W2002262418 @default.
- W4361277650 cites W2027269991 @default.
- W4361277650 cites W2031059176 @default.
- W4361277650 cites W2038894647 @default.
- W4361277650 cites W2078332829 @default.
- W4361277650 cites W2097798909 @default.
- W4361277650 cites W2101754326 @default.
- W4361277650 cites W2111099379 @default.
- W4361277650 cites W2118532317 @default.
- W4361277650 cites W2142676369 @default.
- W4361277650 cites W2152144700 @default.
- W4361277650 cites W2154968323 @default.
- W4361277650 cites W2196899564 @default.
- W4361277650 cites W2413043813 @default.
- W4361277650 cites W2491547442 @default.
- W4361277650 cites W2507000097 @default.
- W4361277650 cites W2538786454 @default.
- W4361277650 cites W2553838260 @default.
- W4361277650 cites W2622774366 @default.
- W4361277650 cites W2772149037 @default.
- W4361277650 cites W2884101170 @default.
- W4361277650 cites W2888484911 @default.
- W4361277650 cites W2904827199 @default.
- W4361277650 cites W2917897282 @default.
- W4361277650 cites W2918748217 @default.
- W4361277650 cites W2921773684 @default.
- W4361277650 cites W2949213644 @default.
- W4361277650 cites W2960979937 @default.
- W4361277650 cites W2964142306 @default.
- W4361277650 cites W2971821708 @default.
- W4361277650 cites W2997651447 @default.
- W4361277650 cites W3002052930 @default.
- W4361277650 cites W3013167866 @default.
- W4361277650 cites W3027849952 @default.
- W4361277650 cites W3098082315 @default.
- W4361277650 cites W3150635270 @default.
- W4361277650 cites W3189237835 @default.
- W4361277650 cites W4200478694 @default.
- W4361277650 cites W4212925316 @default.
- W4361277650 cites W4282960707 @default.
- W4361277650 cites W4293149870 @default.
- W4361277650 cites W4295509862 @default.
- W4361277650 cites W4308022950 @default.
- W4361277650 cites W4310258517 @default.
- W4361277650 cites W4367049822 @default.
- W4361277650 cites W4383868194 @default.
- W4361277650 doi "https://doi.org/10.1101/2023.03.29.534720" @default.
- W4361277650 hasPublicationYear "2023" @default.
- W4361277650 type Work @default.
- W4361277650 citedByCount "1" @default.
- W4361277650 crossrefType "posted-content" @default.
- W4361277650 hasAuthorship W4361277650A5001379247 @default.
- W4361277650 hasAuthorship W4361277650A5016157467 @default.
- W4361277650 hasAuthorship W4361277650A5021382553 @default.
- W4361277650 hasAuthorship W4361277650A5035473961 @default.
- W4361277650 hasAuthorship W4361277650A5078926589 @default.
- W4361277650 hasBestOaLocation W43612776501 @default.
- W4361277650 hasConcept C101762097 @default.
- W4361277650 hasConcept C104317684 @default.
- W4361277650 hasConcept C111936080 @default.
- W4361277650 hasConcept C121608353 @default.
- W4361277650 hasConcept C138885662 @default.
- W4361277650 hasConcept C150194340 @default.
- W4361277650 hasConcept C153911025 @default.
- W4361277650 hasConcept C165864922 @default.
- W4361277650 hasConcept C172313692 @default.
- W4361277650 hasConcept C179926584 @default.
- W4361277650 hasConcept C21784809 @default.
- W4361277650 hasConcept C41895202 @default.
- W4361277650 hasConcept C530470458 @default.
- W4361277650 hasConcept C54355233 @default.
- W4361277650 hasConcept C83640560 @default.
- W4361277650 hasConcept C84606932 @default.
- W4361277650 hasConcept C86339819 @default.
- W4361277650 hasConcept C86803240 @default.
- W4361277650 hasConcept C93946551 @default.
- W4361277650 hasConceptScore W4361277650C101762097 @default.
- W4361277650 hasConceptScore W4361277650C104317684 @default.
- W4361277650 hasConceptScore W4361277650C111936080 @default.
- W4361277650 hasConceptScore W4361277650C121608353 @default.
- W4361277650 hasConceptScore W4361277650C138885662 @default.
- W4361277650 hasConceptScore W4361277650C150194340 @default.
- W4361277650 hasConceptScore W4361277650C153911025 @default.
- W4361277650 hasConceptScore W4361277650C165864922 @default.
- W4361277650 hasConceptScore W4361277650C172313692 @default.
- W4361277650 hasConceptScore W4361277650C179926584 @default.
- W4361277650 hasConceptScore W4361277650C21784809 @default.
- W4361277650 hasConceptScore W4361277650C41895202 @default.