FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4361278752> ?p ?o ?g. }

• W4361278752 endingPage "5857" @default.
• W4361278752 startingPage "5850" @default.
• W4361278752 abstract "In bottom-up proteomic profiling, the complexity of proteome composition and wide dynamic range has created challenges on the limited number of protein identification and proteome coverage, especially in sample input-limited nanoflow (nano) LC–MS/MS analysis. Herein, we developed a fully automatic online 2D nano-LC–MS/MS system using both high-pH and low-pH reverse phase (RP) LCs on a single LC instrument toward comprehensive proteomics analysis. Compared to conventional microflow 2D-LC, the high-pH RP trapping column demonstrated a low sample requirement of cellular protein digest at the μg level with good fractionation resolution of >90% peptides in a single fraction. Compared to the offline 2D RP–RP nano-LC-QTOF using C18-HPLC column and C18-Stage Tip, and 1D nano-LC-QTOF system, superior coverage was observed on the higher number of identified protein groups/unique peptides by 1.35-/1.68-, 1.46-/1.75-, and 3.21-/4.35-fold, respectively, using an online 2D RP–RP nano-LC-QTOF mass spectrometer. On the evolution of quantitation performance, the online 2D high-/low-pH RP data-independent acquisition (DIA) showed a higher reproducibility in protein groups intensity (R2 > 0.977) and more quantified proteins than that obtained using the offline 2D high-/low-pH RP DIA approach. Using an advanced Orbitrap Exploris 480 mass spectrometer, ∼1.9-fold higher proteome coverage was also observed in our 2D online RP–RP system (6039 protein groups) compared to the 1D nano-LC system (3133 protein groups). In summary, the online 2D nano-LC–MS/MS platform can be a sensitive and robust approach compatible with conventional nano-LC instruments for deep proteome coverage of trace amounts of samples." @default.
• W4361278752 created "2023-03-31" @default.
• W4361278752 creator A5017217269 @default.
• W4361278752 creator A5024251430 @default.
• W4361278752 date "2023-03-30" @default.
• W4361278752 modified "2023-10-14" @default.
• W4361278752 title "Online 2D High-pH and Low-pH Reversed-Phase Nano-LC–MS/MS System for Deep Proteome Analysis" @default.
• W4361278752 cites W1167914791 @default.
• W4361278752 cites W1970696723 @default.
• W4361278752 cites W1997945562 @default.
• W4361278752 cites W2009771372 @default.
• W4361278752 cites W2028645231 @default.
• W4361278752 cites W2034356373 @default.
• W4361278752 cites W2041328507 @default.
• W4361278752 cites W2042278775 @default.
• W4361278752 cites W2055922694 @default.
• W4361278752 cites W2062050705 @default.
• W4361278752 cites W2063993329 @default.
• W4361278752 cites W2065578333 @default.
• W4361278752 cites W2068621230 @default.
• W4361278752 cites W2071054574 @default.
• W4361278752 cites W2074027761 @default.
• W4361278752 cites W2117490930 @default.
• W4361278752 cites W2168270559 @default.
• W4361278752 cites W2171874319 @default.
• W4361278752 cites W2316656800 @default.
• W4361278752 cites W2321866757 @default.
• W4361278752 cites W2327092883 @default.
• W4361278752 cites W2737887524 @default.
• W4361278752 cites W2739857744 @default.
• W4361278752 cites W2740267557 @default.
• W4361278752 cites W2883416581 @default.
• W4361278752 cites W2902329895 @default.
• W4361278752 cites W2970757381 @default.
• W4361278752 cites W2997014141 @default.
• W4361278752 cites W3028802693 @default.
• W4361278752 cites W3035751305 @default.
• W4361278752 cites W3139819429 @default.
• W4361278752 cites W3181470757 @default.
• W4361278752 cites W3193029062 @default.
• W4361278752 cites W4210264110 @default.
• W4361278752 doi "https://doi.org/10.1021/acs.analchem.2c02455" @default.
• W4361278752 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36995735" @default.
• W4361278752 hasPublicationYear "2023" @default.
• W4361278752 type Work @default.
• W4361278752 citedByCount "1" @default.
• W4361278752 countsByYear W43612787522023 @default.
• W4361278752 crossrefType "journal-article" @default.
• W4361278752 hasAuthorship W4361278752A5017217269 @default.
• W4361278752 hasAuthorship W4361278752A5024251430 @default.
• W4361278752 hasConcept C104317684 @default.
• W4361278752 hasConcept C104397665 @default.
• W4361278752 hasConcept C113196181 @default.
• W4361278752 hasConcept C162356407 @default.
• W4361278752 hasConcept C185592680 @default.
• W4361278752 hasConcept C2778576202 @default.
• W4361278752 hasConcept C2780841973 @default.
• W4361278752 hasConcept C31827203 @default.
• W4361278752 hasConcept C43617362 @default.
• W4361278752 hasConcept C46111723 @default.
• W4361278752 hasConcept C55493867 @default.
• W4361278752 hasConcept C97428945 @default.
• W4361278752 hasConceptScore W4361278752C104317684 @default.
• W4361278752 hasConceptScore W4361278752C104397665 @default.
• W4361278752 hasConceptScore W4361278752C113196181 @default.
• W4361278752 hasConceptScore W4361278752C162356407 @default.
• W4361278752 hasConceptScore W4361278752C185592680 @default.
• W4361278752 hasConceptScore W4361278752C2778576202 @default.
• W4361278752 hasConceptScore W4361278752C2780841973 @default.
• W4361278752 hasConceptScore W4361278752C31827203 @default.
• W4361278752 hasConceptScore W4361278752C43617362 @default.
• W4361278752 hasConceptScore W4361278752C46111723 @default.
• W4361278752 hasConceptScore W4361278752C55493867 @default.
• W4361278752 hasConceptScore W4361278752C97428945 @default.
• W4361278752 hasFunder F4320322795 @default.
• W4361278752 hasFunder F4320327652 @default.
• W4361278752 hasIssue "14" @default.
• W4361278752 hasLocation W43612787521 @default.
• W4361278752 hasLocation W43612787522 @default.
• W4361278752 hasOpenAccess W4361278752 @default.
• W4361278752 hasPrimaryLocation W43612787521 @default.
• W4361278752 hasRelatedWork W2003261719 @default.
• W4361278752 hasRelatedWork W2070431367 @default.
• W4361278752 hasRelatedWork W2100584122 @default.
• W4361278752 hasRelatedWork W2334381244 @default.
• W4361278752 hasRelatedWork W2371154968 @default.
• W4361278752 hasRelatedWork W2550622545 @default.
• W4361278752 hasRelatedWork W2555382250 @default.
• W4361278752 hasRelatedWork W2885135849 @default.
• W4361278752 hasRelatedWork W4289550775 @default.
• W4361278752 hasRelatedWork W4323833131 @default.
• W4361278752 hasVolume "95" @default.
• W4361278752 isParatext "false" @default.
• W4361278752 isRetracted "false" @default.
• W4361278752 workType "article" @default.