Matches in SemOpenAlex for { <https://semopenalex.org/work/W4361279401> ?p ?o ?g. }
- W4361279401 abstract "In this study, differentially expressed metabolites of vascular endothelial cells were examined to further understand the metabolic regulation of ischemic injury by untargeted metabolomics.Human umbilical vein endothelial cells (HUVECs) were selected to construct an ischemia model using oxygen-glucose deprivation (OGD) and 0, 3, 6, and 9 h of treatment. After that, cell survival levels were determined by CCK8 detection. Flow cytometry, ROS detection, JC-1 detection, and western blotting were used to measure apoptosis and oxidative stress in cells. Then, combined with UPLC Orbitrap/MS, we verified the impacted metabolism pathways by western blotting and RT‒PCR.CCK8 assays showed that the survival of HUVECs was decreased with OGD treatment. Flow cytometry and the expression of cleaved caspase 3 showed that the apoptosis levels of HUVECs increased following OGD treatment. The ROS and JC-1 results further suggested that oxidative stress injury was aggravated. Then, combined with the heatmap, KEGG and IPA, we found that arginine metabolism was differentially altered during different periods of OGD treatment. Furthermore, the expression of four arginine metabolism-related proteins, ASS1, ARG2, ODC1 and SAT1, was found to change during treatment.Arginine metabolism pathway-related proteins were significantly altered by OGD treatment, which suggests that they may have a potential role in ischemic injury." @default.
- W4361279401 created "2023-03-31" @default.
- W4361279401 creator A5019443343 @default.
- W4361279401 creator A5046938617 @default.
- W4361279401 creator A5053204257 @default.
- W4361279401 creator A5064610209 @default.
- W4361279401 creator A5066716873 @default.
- W4361279401 creator A5073480094 @default.
- W4361279401 creator A5088128024 @default.
- W4361279401 date "2023-03-30" @default.
- W4361279401 modified "2023-09-27" @default.
- W4361279401 title "Untargeted metabolomic analysis of ischemic injury in human umbilical vein endothelial cells reveals the involvement of arginine metabolism" @default.
- W4361279401 cites W1530209229 @default.
- W4361279401 cites W1935952230 @default.
- W4361279401 cites W1984355675 @default.
- W4361279401 cites W1990253071 @default.
- W4361279401 cites W1995731129 @default.
- W4361279401 cites W2045290698 @default.
- W4361279401 cites W2061645757 @default.
- W4361279401 cites W2065148256 @default.
- W4361279401 cites W2071745239 @default.
- W4361279401 cites W2093639021 @default.
- W4361279401 cites W2098743872 @default.
- W4361279401 cites W2099694349 @default.
- W4361279401 cites W2102785524 @default.
- W4361279401 cites W2110402304 @default.
- W4361279401 cites W2122091775 @default.
- W4361279401 cites W2127635673 @default.
- W4361279401 cites W2153819921 @default.
- W4361279401 cites W2165681080 @default.
- W4361279401 cites W2237013884 @default.
- W4361279401 cites W2285347461 @default.
- W4361279401 cites W2335390224 @default.
- W4361279401 cites W2387234219 @default.
- W4361279401 cites W2486332948 @default.
- W4361279401 cites W2539494034 @default.
- W4361279401 cites W2554740475 @default.
- W4361279401 cites W2564489970 @default.
- W4361279401 cites W2587537033 @default.
- W4361279401 cites W2591053022 @default.
- W4361279401 cites W2737572512 @default.
- W4361279401 cites W2888497643 @default.
- W4361279401 cites W2901716735 @default.
- W4361279401 cites W2902246840 @default.
- W4361279401 cites W2914278074 @default.
- W4361279401 cites W2918042433 @default.
- W4361279401 cites W2947593497 @default.
- W4361279401 cites W3037803867 @default.
- W4361279401 cites W3041579768 @default.
- W4361279401 cites W3095316811 @default.
- W4361279401 cites W3125630250 @default.
- W4361279401 cites W3127601733 @default.
- W4361279401 cites W3133506179 @default.
- W4361279401 cites W3136668199 @default.
- W4361279401 cites W3164293639 @default.
- W4361279401 cites W3177789874 @default.
- W4361279401 cites W3181510010 @default.
- W4361279401 cites W3215014564 @default.
- W4361279401 cites W4205089556 @default.
- W4361279401 cites W4206498374 @default.
- W4361279401 cites W4210242902 @default.
- W4361279401 cites W4225567734 @default.
- W4361279401 cites W4242045705 @default.
- W4361279401 cites W4283017342 @default.
- W4361279401 cites W4285592755 @default.
- W4361279401 cites W4289839110 @default.
- W4361279401 cites W4290739774 @default.
- W4361279401 doi "https://doi.org/10.1186/s12986-023-00737-0" @default.
- W4361279401 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36998018" @default.
- W4361279401 hasPublicationYear "2023" @default.
- W4361279401 type Work @default.
- W4361279401 citedByCount "0" @default.
- W4361279401 crossrefType "journal-article" @default.
- W4361279401 hasAuthorship W4361279401A5019443343 @default.
- W4361279401 hasAuthorship W4361279401A5046938617 @default.
- W4361279401 hasAuthorship W4361279401A5053204257 @default.
- W4361279401 hasAuthorship W4361279401A5064610209 @default.
- W4361279401 hasAuthorship W4361279401A5066716873 @default.
- W4361279401 hasAuthorship W4361279401A5073480094 @default.
- W4361279401 hasAuthorship W4361279401A5088128024 @default.
- W4361279401 hasBestOaLocation W43612794011 @default.
- W4361279401 hasConcept C104317684 @default.
- W4361279401 hasConcept C153911025 @default.
- W4361279401 hasConcept C185592680 @default.
- W4361279401 hasConcept C190283241 @default.
- W4361279401 hasConcept C202751555 @default.
- W4361279401 hasConcept C21565614 @default.
- W4361279401 hasConcept C2776151105 @default.
- W4361279401 hasConcept C2777411675 @default.
- W4361279401 hasConcept C2777468819 @default.
- W4361279401 hasConcept C48349386 @default.
- W4361279401 hasConcept C515207424 @default.
- W4361279401 hasConcept C553184892 @default.
- W4361279401 hasConcept C55493867 @default.
- W4361279401 hasConcept C60644358 @default.
- W4361279401 hasConcept C62231903 @default.
- W4361279401 hasConcept C85265743 @default.
- W4361279401 hasConcept C86803240 @default.
- W4361279401 hasConcept C95444343 @default.
- W4361279401 hasConcept C98274493 @default.