FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4361283440> ?p ?o ?g. }

• W4361283440 endingPage "116399" @default.
• W4361283440 startingPage "116399" @default.
• W4361283440 abstract "Tiger bone, which had long been used in traditional Chinese medicine, had the action of removing wind and alleviating pain, strengthening the sinews and bones, and often used to treat bone impediment, and atrophic debility of bones in TCM clinical practice. As a substitute of natural bone tiger, artificial tiger bone Jintiange (JTG), has been approved by the State Food and Drug Administration of China for relief the symptom of osteoporosis, such as lumbago and back pain, lassitude in loin and legs, flaccidity and weakness legs, and walk with difficulty based on TCM theory. JTG has similar chemical profile to natural tiger bone, and contains mineral substance, peptides and proteins, and has been shown to protect bone loss in ovariectomized mice and exert the regulatory effects on osteoblast and osteoclast activities. But how the peptides and proteins in JTG modulate bone formation remains unclear.To investigate the stimulating effects of JTG proteins on osteogenesis and explore the possible underlying mechanisms.JTG proteins were prepared from JTG Capsules by extracting calcium, phosphorus and other inorganic elements using SEP-PaktC18 desalting column. MC3T3-E1 cells were treated with JTG proteins to evaluate their effects and explore the underlying mechanisms. Osteoblast proliferation was detected by CCK-8 method. ALP activity was detected using a relevant assay kit, and bone mineralized nodules were stained with alizarin red-Tris-HCl solution. Cell apoptosis was analyzed by flow cytometry. Autophagy was observed by MDC staining, and autophagosomes were observed by TEM. Nuclear translocations of LC3 and CHOP were detected by immunofluorescence and observed under a laser confocal microscope. The expression of key proteins related to osteogenesis, apoptosis, autophagy and PI3K/AKT and ER stress pathways was analyzed by Western Blot analysis.JTG proteins improved osteogenesis as evidenced by the alteration of proliferation, differentiation and mineralization of MC3T3-E1 osteoblasts, inhibited their apoptosis, and enhanced autophagosome formation and autophagy. They also regulated the expression of key proteins of PI3K/AKT and ER stress pathways. In addition, PI3K/AKT and ER stress pathway inhibitors could reverse the regulatory effects of JTG proteins on osteogenesis, apoptosis, autophagy and PI3K/AKT and ER stress pathways.JTG proteins increased the osteogenesis and inhibited osteoblast apoptosis by enhancing autophagy via PI3K/AKT and ER stress signaling pathways." @default.
• W4361283440 created "2023-03-31" @default.
• W4361283440 creator A5005847066 @default.
• W4361283440 creator A5011792514 @default.
• W4361283440 creator A5018893156 @default.
• W4361283440 creator A5035562422 @default.
• W4361283440 creator A5049481678 @default.
• W4361283440 creator A5069583041 @default.
• W4361283440 creator A5072001815 @default.
• W4361283440 creator A5072090145 @default.
• W4361283440 creator A5077529888 @default.
• W4361283440 creator A5090128675 @default.
• W4361283440 creator A5090728786 @default.
• W4361283440 date "2023-07-01" @default.
• W4361283440 modified "2023-09-26" @default.
• W4361283440 title "Jintiange proteins promote osteogenesis and inhibit apoptosis of osteoblasts by enhancing autophagy via PI3K/AKT and ER stress pathways" @default.
• W4361283440 cites W2045970460 @default.
• W4361283440 cites W2067785289 @default.
• W4361283440 cites W2783606239 @default.
• W4361283440 cites W2904120323 @default.
• W4361283440 cites W2919577856 @default.
• W4361283440 cites W2937078475 @default.
• W4361283440 cites W2945170725 @default.
• W4361283440 cites W2954164553 @default.
• W4361283440 cites W2955204606 @default.
• W4361283440 cites W2968952977 @default.
• W4361283440 cites W2989544643 @default.
• W4361283440 cites W2989803368 @default.
• W4361283440 cites W2994125455 @default.
• W4361283440 cites W3007541218 @default.
• W4361283440 cites W3013315583 @default.
• W4361283440 cites W3041068253 @default.
• W4361283440 cites W3041591569 @default.
• W4361283440 cites W3092556360 @default.
• W4361283440 cites W3093595738 @default.
• W4361283440 cites W3107036058 @default.
• W4361283440 cites W3118300923 @default.
• W4361283440 cites W3122919452 @default.
• W4361283440 cites W3130118686 @default.
• W4361283440 cites W3132921052 @default.
• W4361283440 cites W3133725907 @default.
• W4361283440 cites W3139209394 @default.
• W4361283440 cites W3164231153 @default.
• W4361283440 cites W3164752546 @default.
• W4361283440 cites W3170983871 @default.
• W4361283440 cites W3173562351 @default.
• W4361283440 cites W3187238766 @default.
• W4361283440 cites W3187280852 @default.
• W4361283440 cites W3204539006 @default.
• W4361283440 cites W3208037990 @default.
• W4361283440 cites W3211679955 @default.
• W4361283440 cites W3211820869 @default.
• W4361283440 cites W3212359110 @default.
• W4361283440 cites W4200232049 @default.
• W4361283440 cites W4200482666 @default.
• W4361283440 cites W4200537902 @default.
• W4361283440 cites W4210645998 @default.
• W4361283440 cites W4224222767 @default.
• W4361283440 cites W4225261086 @default.
• W4361283440 cites W4285810202 @default.
• W4361283440 cites W4288068453 @default.
• W4361283440 cites W4292884074 @default.
• W4361283440 cites W4296385718 @default.
• W4361283440 cites W4301372146 @default.
• W4361283440 doi "https://doi.org/10.1016/j.jep.2023.116399" @default.
• W4361283440 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36997131" @default.
• W4361283440 hasPublicationYear "2023" @default.
• W4361283440 type Work @default.
• W4361283440 citedByCount "1" @default.
• W4361283440 countsByYear W43612834402023 @default.
• W4361283440 crossrefType "journal-article" @default.
• W4361283440 hasAuthorship W4361283440A5005847066 @default.
• W4361283440 hasAuthorship W4361283440A5011792514 @default.
• W4361283440 hasAuthorship W4361283440A5018893156 @default.
• W4361283440 hasAuthorship W4361283440A5035562422 @default.
• W4361283440 hasAuthorship W4361283440A5049481678 @default.
• W4361283440 hasAuthorship W4361283440A5069583041 @default.
• W4361283440 hasAuthorship W4361283440A5072001815 @default.
• W4361283440 hasAuthorship W4361283440A5072090145 @default.
• W4361283440 hasAuthorship W4361283440A5077529888 @default.
• W4361283440 hasAuthorship W4361283440A5090128675 @default.
• W4361283440 hasAuthorship W4361283440A5090728786 @default.
• W4361283440 hasConcept C126322002 @default.
• W4361283440 hasConcept C170493617 @default.
• W4361283440 hasConcept C185592680 @default.
• W4361283440 hasConcept C190283241 @default.
• W4361283440 hasConcept C202751555 @default.
• W4361283440 hasConcept C203522944 @default.
• W4361283440 hasConcept C2776033226 @default.
• W4361283440 hasConcept C2777164284 @default.
• W4361283440 hasConcept C2778260815 @default.
• W4361283440 hasConcept C55493867 @default.
• W4361283440 hasConcept C71924100 @default.
• W4361283440 hasConcept C8264082 @default.
• W4361283440 hasConcept C86554907 @default.
• W4361283440 hasConcept C98274493 @default.
• W4361283440 hasConceptScore W4361283440C126322002 @default.
• W4361283440 hasConceptScore W4361283440C170493617 @default.

• next