FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4361284906> ?p ?o ?g. }

• W4361284906 endingPage "2235" @default.
• W4361284906 startingPage "2223" @default.
• W4361284906 abstract "BackgroundA disintegrin and metalloprotease 17 (ADAM17) catalyzes platelet glycoprotein (GP) Ibα ectodomain shedding, thereby releasing glycocalicin in plasma. The spatiotemporal control over the enzyme-substrate interaction and the biological consequences of GPIbα shedding are poorly understood.ObjectivesThis study aimed to determine the spatiotemporal control over GPIbα shedding by ADAM17.MethodsTransmission electron microscopy with immunogold staining, immunoprecipitation, and quantitative western blotting were used.ResultsImmunogold staining showed that all ADAM17 antigen is expressed intracellularly, irrespective of platelet activation. ADAM17 clustered in patches on a tortuous membrane system different from α- and dense granules. Mild activation by platelet adhesion to immobilized fibrinogen did not cause GPIbα shedding, whereas strong and sustained stimulation using thrombin and collagen (analogs) did. Glycocalicin release kinetics was considerably slower than typical hemostasis, starting at 20 minutes and reaching a plateau after 3 hours of strong stimulation. Inhibition of the ADAM17 scissile bond specifically in GPIbα receptors that reside on the platelet’s extracellular surface did not prevent shedding, which is in line with the strict intracellular location of ADAM17. Instead, shedding was restricted to a large GPIbα subpopulation that is inaccessible on resting platelets but becomes partially accessible following platelet stimulation. Furthermore, the data show that proteinaceous, water-soluble ADAM17 inhibitors cannot inhibit GPIbα shedding, whereas membrane permeable small molecule ADAM inhibitors can.ConclusionThe data show that platelets harbor 2 distinct GPIbα subpopulations: one that presents at the platelet’s surface known for its role in primary hemostasis and one that provides substrate for proteolysis by ADAM17 with kinetics that suggest a role beyond hemostasis." @default.
• W4361284906 created "2023-03-31" @default.
• W4361284906 creator A5036900302 @default.
• W4361284906 creator A5049047494 @default.
• W4361284906 creator A5050164175 @default.
• W4361284906 creator A5052052624 @default.
• W4361284906 creator A5073486104 @default.
• W4361284906 creator A5082799541 @default.
• W4361284906 creator A5086939300 @default.
• W4361284906 date "2023-08-01" @default.
• W4361284906 modified "2023-10-01" @default.
• W4361284906 title "GPIbα shedding in platelets is controlled by strict intracellular containment of both enzyme and substrate" @default.
• W4361284906 cites W131528519 @default.
• W4361284906 cites W1707922975 @default.
• W4361284906 cites W1883664674 @default.
• W4361284906 cites W1971124929 @default.
• W4361284906 cites W1980661258 @default.
• W4361284906 cites W1981273659 @default.
• W4361284906 cites W1992358929 @default.
• W4361284906 cites W2005985968 @default.
• W4361284906 cites W2009802325 @default.
• W4361284906 cites W2011165507 @default.
• W4361284906 cites W2020724202 @default.
• W4361284906 cites W2021183609 @default.
• W4361284906 cites W2024402718 @default.
• W4361284906 cites W2045165700 @default.
• W4361284906 cites W2050810522 @default.
• W4361284906 cites W2053345485 @default.
• W4361284906 cites W2055588429 @default.
• W4361284906 cites W2061943904 @default.
• W4361284906 cites W2063851500 @default.
• W4361284906 cites W2087754560 @default.
• W4361284906 cites W2114259322 @default.
• W4361284906 cites W2119445810 @default.
• W4361284906 cites W2123077415 @default.
• W4361284906 cites W2126961090 @default.
• W4361284906 cites W2140639765 @default.
• W4361284906 cites W2141905650 @default.
• W4361284906 cites W2142166517 @default.
• W4361284906 cites W2153991213 @default.
• W4361284906 cites W2156133814 @default.
• W4361284906 cites W2283163427 @default.
• W4361284906 cites W2411216637 @default.
• W4361284906 cites W2413277520 @default.
• W4361284906 cites W2413515352 @default.
• W4361284906 cites W2492767537 @default.
• W4361284906 cites W2524775321 @default.
• W4361284906 cites W2529226582 @default.
• W4361284906 cites W2538622663 @default.
• W4361284906 cites W2566924442 @default.
• W4361284906 cites W2619744402 @default.
• W4361284906 cites W2736267104 @default.
• W4361284906 cites W2789023287 @default.
• W4361284906 cites W2790752610 @default.
• W4361284906 cites W2809653028 @default.
• W4361284906 cites W2891851394 @default.
• W4361284906 cites W2896942841 @default.
• W4361284906 cites W2901041585 @default.
• W4361284906 cites W2952019293 @default.
• W4361284906 cites W2981036457 @default.
• W4361284906 cites W2981367456 @default.
• W4361284906 cites W2981717785 @default.
• W4361284906 cites W91999869 @default.
• W4361284906 doi "https://doi.org/10.1016/j.jtha.2023.03.020" @default.
• W4361284906 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37001816" @default.
• W4361284906 hasPublicationYear "2023" @default.
• W4361284906 type Work @default.
• W4361284906 citedByCount "1" @default.
• W4361284906 countsByYear W43612849062023 @default.
• W4361284906 crossrefType "journal-article" @default.
• W4361284906 hasAuthorship W4361284906A5036900302 @default.
• W4361284906 hasAuthorship W4361284906A5049047494 @default.
• W4361284906 hasAuthorship W4361284906A5050164175 @default.
• W4361284906 hasAuthorship W4361284906A5052052624 @default.
• W4361284906 hasAuthorship W4361284906A5073486104 @default.
• W4361284906 hasAuthorship W4361284906A5082799541 @default.
• W4361284906 hasAuthorship W4361284906A5086939300 @default.
• W4361284906 hasBestOaLocation W43612849061 @default.
• W4361284906 hasConcept C1009742 @default.
• W4361284906 hasConcept C108625454 @default.
• W4361284906 hasConcept C12554922 @default.
• W4361284906 hasConcept C126322002 @default.
• W4361284906 hasConcept C159654299 @default.
• W4361284906 hasConcept C170493617 @default.
• W4361284906 hasConcept C181199279 @default.
• W4361284906 hasConcept C185592680 @default.
• W4361284906 hasConcept C203014093 @default.
• W4361284906 hasConcept C26828662 @default.
• W4361284906 hasConcept C2777292125 @default.
• W4361284906 hasConcept C2778259572 @default.
• W4361284906 hasConcept C2778589496 @default.
• W4361284906 hasConcept C2780573682 @default.
• W4361284906 hasConcept C28406088 @default.
• W4361284906 hasConcept C2910597130 @default.
• W4361284906 hasConcept C30030713 @default.
• W4361284906 hasConcept C3018697912 @default.
• W4361284906 hasConcept C55493867 @default.
• W4361284906 hasConcept C55728118 @default.

• next