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- W4361303133 endingPage "101" @default.
- W4361303133 startingPage "53" @default.
- W4361303133 abstract "Genetically engineered mice are used as avatars to understand mammalian gene function and develop therapies for human disease. During genetic modification, unintended changes can occur, and these changes may result in misassigned gene-phenotype relationships leading to incorrect or incomplete experimental interpretations. The types of unintended changes that may occur depend on the allele type being made and the genetic engineering approach used. Here we broadly categorize allele types as deletions, insertions, base changes, and transgenes derived from engineered embryonic stem (ES) cells or edited mouse embryos. However, the methods we describe can be adapted to other allele types and engineering strategies. We describe the sources and consequ ences of common unintended changes and best practices for detecting both intended and unintended changes by screening and genetic and molecular quality control (QC) of chimeras, founders, and their progeny. Employing these practices, along with careful allele design and good colony management, will increase the chance that investigations using genetically engineered mice will produce high-quality reproducible results, to enable a robust understanding of gene function, human disease etiology, and therapeutic development." @default.
- W4361303133 created "2023-03-31" @default.
- W4361303133 creator A5012524033 @default.
- W4361303133 creator A5018268251 @default.
- W4361303133 date "2023-01-01" @default.
- W4361303133 modified "2023-09-30" @default.
- W4361303133 title "Genetic and Molecular Quality Control of Genetically Engineered Mice" @default.
- W4361303133 cites W1504564486 @default.
- W4361303133 cites W1555684022 @default.
- W4361303133 cites W1559371943 @default.
- W4361303133 cites W1638907598 @default.
- W4361303133 cites W1768274470 @default.
- W4361303133 cites W1963687563 @default.
- W4361303133 cites W1964378686 @default.
- W4361303133 cites W1964668995 @default.
- W4361303133 cites W1965000349 @default.
- W4361303133 cites W1968923276 @default.
- W4361303133 cites W1970669095 @default.
- W4361303133 cites W1977397104 @default.
- W4361303133 cites W1977958298 @default.
- W4361303133 cites W1982267895 @default.
- W4361303133 cites W1989257894 @default.
- W4361303133 cites W1989873812 @default.
- W4361303133 cites W1991269933 @default.
- W4361303133 cites W1995049749 @default.
- W4361303133 cites W1995479176 @default.
- W4361303133 cites W1997536598 @default.
- W4361303133 cites W2007552905 @default.
- W4361303133 cites W2009482935 @default.
- W4361303133 cites W2020054949 @default.
- W4361303133 cites W2023545713 @default.
- W4361303133 cites W2023630351 @default.
- W4361303133 cites W2025127209 @default.
- W4361303133 cites W2027666734 @default.
- W4361303133 cites W2027937017 @default.
- W4361303133 cites W2030993769 @default.
- W4361303133 cites W2032019813 @default.
- W4361303133 cites W2034254870 @default.
- W4361303133 cites W2036176224 @default.
- W4361303133 cites W2047925113 @default.
- W4361303133 cites W2049685571 @default.
- W4361303133 cites W2050480410 @default.
- W4361303133 cites W2052686571 @default.
- W4361303133 cites W2056030272 @default.
- W4361303133 cites W2060883703 @default.
- W4361303133 cites W2065189089 @default.
- W4361303133 cites W2085544097 @default.
- W4361303133 cites W2085925156 @default.
- W4361303133 cites W2086250047 @default.
- W4361303133 cites W2087049199 @default.
- W4361303133 cites W2090020789 @default.
- W4361303133 cites W2099259774 @default.
- W4361303133 cites W2102347283 @default.
- W4361303133 cites W2103675845 @default.
- W4361303133 cites W2103740237 @default.
- W4361303133 cites W2107517033 @default.
- W4361303133 cites W2112444085 @default.
- W4361303133 cites W2115603949 @default.
- W4361303133 cites W2116534141 @default.
- W4361303133 cites W2118887202 @default.
- W4361303133 cites W2120049846 @default.
- W4361303133 cites W2133661231 @default.
- W4361303133 cites W2140754284 @default.
- W4361303133 cites W2154331071 @default.
- W4361303133 cites W2157176404 @default.
- W4361303133 cites W2158634868 @default.
- W4361303133 cites W2159358696 @default.
- W4361303133 cites W2162098634 @default.
- W4361303133 cites W2170039745 @default.
- W4361303133 cites W2173737475 @default.
- W4361303133 cites W2209539687 @default.
- W4361303133 cites W2256109864 @default.
- W4361303133 cites W2409121249 @default.
- W4361303133 cites W2412316478 @default.
- W4361303133 cites W2418866759 @default.
- W4361303133 cites W2513453849 @default.
- W4361303133 cites W2518901523 @default.
- W4361303133 cites W2554763217 @default.
- W4361303133 cites W2562311664 @default.
- W4361303133 cites W2562446577 @default.
- W4361303133 cites W2566283739 @default.
- W4361303133 cites W2568441540 @default.
- W4361303133 cites W2614850398 @default.
- W4361303133 cites W2620568304 @default.
- W4361303133 cites W2671308999 @default.
- W4361303133 cites W2727344742 @default.
- W4361303133 cites W2735132087 @default.
- W4361303133 cites W2741508285 @default.
- W4361303133 cites W2762722939 @default.
- W4361303133 cites W2769551771 @default.
- W4361303133 cites W2782551527 @default.
- W4361303133 cites W2794894092 @default.
- W4361303133 cites W2795791084 @default.
- W4361303133 cites W2799977176 @default.
- W4361303133 cites W2803557679 @default.
- W4361303133 cites W2809309743 @default.
- W4361303133 cites W2809315771 @default.
- W4361303133 cites W2883697525 @default.