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• W4361762542 abstract "Objective: To establish a DNA detection platform based on a tapered optical fiber to detect Leptospira DNA by targeting the leptospiral secY gene.Methods: The biosensor works on the principle of light propagating in the special geometry of the optical fiber tapered from a waist diameter of 125 to 12 µm. The fiber surface was functionalized through a cascade of chemical treatments and the immobilization of a DNA capture probe targeting the secY gene. The presence of the target DNA was determined from the wavelength shift in the optical transmission spectrum.Results: The biosensor demonstrated good sensitivity, detecting Leptospira DNA at 0.001 ng/µL, and was selective for Leptospira DNA without cross-reactivity with non-leptospiral microorganisms. The biosensor specifically detected DNA that was specifically amplified through the loop-mediated isothermal amplification approach.Conclusions: These findings warrant the potential of this platform to be developed as a novel alternative approach to diagnose leptospirosis." @default.
• W4361762542 created "2023-04-04" @default.
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• W4361762542 date "2023-01-01" @default.
• W4361762542 modified "2023-10-18" @default.
• W4361762542 title "Tapered optical fiber DNA biosensor for detecting Leptospira DNA" @default.
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• W4361762542 doi "https://doi.org/10.4103/1995-7645.372293" @default.
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