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• W4361842304 abstract "<div>Abstract<p>Pancreatic ductal adenocarcinoma (PDAC) is an aggressive malignancy characterized by extensive local invasion and systemic spread. In this study, we employed a three-dimensional organoid model of human pancreatic cancer to characterize the molecular alterations critical for invasion. Time-lapse microscopy was used to observe invasion in organoids from 25 surgically resected human PDAC samples in collagen I. Subsequent lentiviral modification and small-molecule inhibitors were used to investigate the molecular programs underlying invasion in PDAC organoids. When cultured in collagen I, PDAC organoids exhibited two distinct, morphologically defined invasive phenotypes, mesenchymal and collective. Each individual PDAC gave rise to organoids with a predominant phenotype, and PDAC that generated organoids with predominantly mesenchymal invasion showed a worse prognosis. Collective invasion predominated in organoids from cancers with somatic mutations in the driver gene <i>SMAD4</i> (or its signaling partner <i>TGFBR2</i>). Reexpression of SMAD4 abrogated the collective invasion phenotype in <i>SMAD4</i>-mutant PDAC organoids, indicating that SMAD4 loss is required for collective invasion in PDAC organoids. Surprisingly, invasion in passaged <i>SMAD4</i>-mutant PDAC organoids required exogenous TGFβ, suggesting that invasion in <i>SMAD4</i>-mutant organoids is mediated through noncanonical TGFβ signaling. The Rho-like GTPases RAC1 and CDC42 acted as potential mediators of TGFβ-stimulated invasion in <i>SMAD4</i>-mutant PDAC organoids, as inhibition of these GTPases suppressed collective invasion in our model. These data suggest that PDAC utilizes different invasion programs depending on <i>SMAD4</i> status, with collective invasion uniquely present in PDAC with SMAD4 loss.</p>Significance:<p>Organoid models of PDAC highlight the importance of SMAD4 loss in invasion, demonstrating that invasion programs in <i>SMAD4</i>-mutant and <i>SMAD4</i> wild-type tumors are different in both morphology and molecular mechanism.</p></div>" @default.
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• W4361842304 date "2023-03-31" @default.
• W4361842304 modified "2023-10-18" @default.
• W4361842304 title "Data from Pattern of Invasion in Human Pancreatic Cancer Organoids Is Associated with Loss of SMAD4 and Clinical Outcome" @default.
• W4361842304 doi "https://doi.org/10.1158/0008-5472.c.6511710.v1" @default.
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