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- W4361936632 abstract "<p>Supplementary Figure 3. Chemerin incubation has no significant effect on tumor cell apoptosis. DU145 tumor cells were treated with PBS or 6nM chemerin concentration for 48h. Following initial incubation, cells were continued to be treated for 24h with PBS or chemerin with or without IFN-γ. An Annexin-V FITC antibody was used to stain cells going through apoptosis and 7-AAD stained each cell in the total cell population. Using three independent experiments, triplicate tubes of each cell subset were assessed via FACS (n = 3). A. Representative flow cytometry plots showing cells stained with 7-AAD only, Annexin-V FITC only, and a representative sample stained with both 7-AAD vs Annexin-V FITC. Graph of quantitative apoptosis results over 3 independent experiments. There were no significant difference in apoptosis between the PBS vs 6nM chemerin treated cells. (n = 3) B. Quantitative graph showing caspase-3/-7 expression in the control PBS vs chemerin treated cells following a 72h incubation with vehicle control (PBS) or 6nM chemerin (n = 3 independent sample sets). These results show that chemerin does not affect overall cell apoptosis or directly related caspase-3/-7 expression for 72h total incubation in all four cell lines.</p>" @default.
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- W4361936632 date "2023-03-31" @default.
- W4361936632 modified "2023-09-25" @default.
- W4361936632 title "Supplementary Figure 3 from Chemerin Reactivates PTEN and Suppresses PD-L1 in Tumor Cells via Modulation of a Novel CMKLR1-mediated Signaling Cascade" @default.
- W4361936632 doi "https://doi.org/10.1158/1078-0432.22474863.v1" @default.
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