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- W4361948901 abstract "<div>Abstract<p><b>Purpose:</b> The Na/I symporter (hNIS) promotes concentration of iodine in cells. In cancer gene therapy, this transgene has potential as a reporter gene for molecular imaging of viral biodistribution and as a therapeutic protein promoting <sup>131</sup>I-mediated radiotherapy. Here, we combined the imaging and therapeutic potential of hNIS in an oncolytic adenoviruses targeting colorectal cancer cells.</p><p><b>Experimental Design:</b> We generated an adenovirus (AdIP2) encoding <i>hNIS</i> and capable of selective replication in colorectal carcinoma cells. The selectivity of this virus was verified <i>in vitro</i> and <i>in vivo</i>. Its spread in tumors was monitored <i>in vivo</i> using single-photon emission computed tomography/CT imaging upon <sup>99m</sup>TcO<sub>4</sub><sup>−</sup> injection and confirmed by immunohistochemistry. Metabolic radiotherapy was done through injection of therapeutic doses of <sup>131</sup>I<sup>−</sup>.</p><p><b>Results:</b> We showed <i>in vitro</i> and <i>in vivo</i> the selectivity of AdIP2 and that <i>hNIS</i> expression is restricted to the target cells. Imaging and immunohistochemical data showed that viral spread is limited and that the point of maximal <i>hNIS</i> expression is reached 48 hours after a single intratumoral injection. Administration of a single therapeutic dose of <sup>131</sup>I at this time point led to a dramatic reduction in tumor size not observed in <i>hNIS</i>-negative viruses.</p><p><b>Conclusions:</b> This report showed for the first time that the combination of the imaging and therapeutic potentials of <i>hNIS</i> can be applied to oncolytic adenoviruses in experimental models of cancer. (Clin Cancer Res 2009;15(21):6595–601)</p></div>" @default.
- W4361948901 created "2023-04-05" @default.
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- W4361948901 date "2023-03-31" @default.
- W4361948901 modified "2023-10-14" @default.
- W4361948901 title "Data from Targeted Radionuclide Therapy Using a Wnt-Targeted Replicating Adenovirus Encoding the Na/I Symporter" @default.
- W4361948901 doi "https://doi.org/10.1158/1078-0432.c.6517387.v1" @default.
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