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• W4361951567 abstract "<div>Abstract<p><b>Purpose:</b> The inability to visualize cancer during prostatectomy contributes to positive margins, cancer recurrence, and surgical side effects. A molecularly targeted fluorescent probe offers the potential for real-time intraoperative imaging. The goal of this study was to develop a probe for image-guided prostate cancer surgery.</p><p><b>Experimental Design:</b> An antibody fragment (cys-diabody, cDb) against prostate stem cell antigen (PSCA) was conjugated to a far-red fluorophore, Cy5. The integrity and binding of the probe to PSCA was confirmed by gel electrophoresis, size exclusion, and flow cytometry, respectively. Subcutaneous models of PSCA-expressing xenografts were used to assess the biodistribution and <i>in vivo</i> kinetics, whereas an invasive intramuscular model was utilized to explore the performance of Cy5-cDb–mediated fluorescence guidance in representative surgical scenarios. Finally, a prospective, randomized study comparing surgical resection with and without fluorescent guidance was performed to determine whether this probe could reduce the incidence of positive margins.</p><p><b>Results:</b> Cy5-cDb demonstrated excellent purity, stability, and specific binding to PSCA. <i>In vivo</i> imaging showed maximal signal-to-background ratios at 6 hours. In mice carrying PSCA⁺ and negative (−) dual xenografts, the mean fluorescence ratio of PSCA^+/− tumors was 4.4:1. In surgical resection experiments, residual tumors <1 mm that were missed on white light surgery were identified and resected using fluorescence guidance, which reduced the incidence of positive surgical margins (0/8) compared with white light surgery alone (7/7).</p><p><b>Conclusions:</b> Fluorescently labeled cDb enables real-time <i>in vivo</i> imaging of prostate cancer xenografts in mice, and facilitates more complete tumor removal than conventional white light surgery alone. <i>Clin Cancer Res; 22(6); 1403–12. ©2015 AACR</i>.</p><p><i>See related commentary by van Leeuwen and van der Poel, p. 1304</i></p></div>" @default.
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• W4361951567 date "2023-03-31" @default.
• W4361951567 modified "2023-09-25" @default.
• W4361951567 title "Data from Fluorescent Image–Guided Surgery with an Anti-Prostate Stem Cell Antigen (PSCA) Diabody Enables Targeted Resection of Mouse Prostate Cancer Xenografts in Real Time" @default.
• W4361951567 doi "https://doi.org/10.1158/1078-0432.c.6523863.v1" @default.
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