Matches in SemOpenAlex for { <https://semopenalex.org/work/W4361962493> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W4361962493 abstract "<p>Supplementary Figure 5. Targeted inhibitors and CMKLR1 Blocking. A. mTOR inhibitor RAD001 (everolimus; 200nM, Selleck Chemical) was used to treat DU145 tumor cells prior to chemerin exposure, and levels of indicated proteins assessed by Western blot; RAD001 suppressed expression of both pS6 and PD-L1, regardless of the presence or absence of chemerin. Taken together, these data suggest that chemerin treatment results in an increase in tumor PTEN expression, with associated changes in components of the canonical PI3K-AKT-mTOR signaling pathway as well as decreases in pS6/S6 and PD-L1 expression, comparable to PI3K and mTOR inhibitors that have been used in clinical trials. B. Flow cytometry of DU145 cells pretreated with either alpha-NETA or the blocking peptide showed >50% reduction in the binding of an anti-CMKLR1 monoclonal antibody (FAB362P, R&D Systems) compared to controls, indicating specific inhibition of CMKLR1 by these agents. Figures representative of n=2 experiments each; *p < 0.05, NS=not significant. C. A CMKLR1 blocking peptide (5uM, sc-374570 P, Santa Cruz Biotechnology) was used to treat DU145 cells in the presence or absence of chemerin. A representative western blot (left) shows a significant increase of PTEN and decrease of PD-L1 expression seen with chemerin treatment was completely abrogated in cells pretreated with the anti-CMKLR1 peptide. Western results from 3 replicates are quantified for PTEN and PD-L1 expression (right). (*p < 0.05, n = 3).</p>" @default.
- W4361962493 created "2023-04-05" @default.
- W4361962493 creator A5013255341 @default.
- W4361962493 creator A5033506869 @default.
- W4361962493 creator A5066433776 @default.
- W4361962493 creator A5076485897 @default.
- W4361962493 creator A5083027775 @default.
- W4361962493 date "2023-03-31" @default.
- W4361962493 modified "2023-09-25" @default.
- W4361962493 title "Supplementary Figure 5 from Chemerin Reactivates PTEN and Suppresses PD-L1 in Tumor Cells via Modulation of a Novel CMKLR1-mediated Signaling Cascade" @default.
- W4361962493 doi "https://doi.org/10.1158/1078-0432.22474854.v1" @default.
- W4361962493 hasPublicationYear "2023" @default.
- W4361962493 type Work @default.
- W4361962493 citedByCount "0" @default.
- W4361962493 crossrefType "posted-content" @default.
- W4361962493 hasAuthorship W4361962493A5013255341 @default.
- W4361962493 hasAuthorship W4361962493A5033506869 @default.
- W4361962493 hasAuthorship W4361962493A5066433776 @default.
- W4361962493 hasAuthorship W4361962493A5076485897 @default.
- W4361962493 hasAuthorship W4361962493A5083027775 @default.
- W4361962493 hasBestOaLocation W43619624931 @default.
- W4361962493 hasConcept C104317684 @default.
- W4361962493 hasConcept C121608353 @default.
- W4361962493 hasConcept C126322002 @default.
- W4361962493 hasConcept C134018914 @default.
- W4361962493 hasConcept C153911025 @default.
- W4361962493 hasConcept C185592680 @default.
- W4361962493 hasConcept C194832188 @default.
- W4361962493 hasConcept C199901988 @default.
- W4361962493 hasConcept C2776415932 @default.
- W4361962493 hasConcept C2776438761 @default.
- W4361962493 hasConcept C2777391703 @default.
- W4361962493 hasConcept C2777609662 @default.
- W4361962493 hasConcept C2778308172 @default.
- W4361962493 hasConcept C2779306644 @default.
- W4361962493 hasConcept C2779723316 @default.
- W4361962493 hasConcept C502942594 @default.
- W4361962493 hasConcept C55493867 @default.
- W4361962493 hasConcept C62478195 @default.
- W4361962493 hasConcept C71924100 @default.
- W4361962493 hasConcept C75217442 @default.
- W4361962493 hasConcept C86554907 @default.
- W4361962493 hasConcept C86803240 @default.
- W4361962493 hasConcept C95444343 @default.
- W4361962493 hasConcept C96232424 @default.
- W4361962493 hasConceptScore W4361962493C104317684 @default.
- W4361962493 hasConceptScore W4361962493C121608353 @default.
- W4361962493 hasConceptScore W4361962493C126322002 @default.
- W4361962493 hasConceptScore W4361962493C134018914 @default.
- W4361962493 hasConceptScore W4361962493C153911025 @default.
- W4361962493 hasConceptScore W4361962493C185592680 @default.
- W4361962493 hasConceptScore W4361962493C194832188 @default.
- W4361962493 hasConceptScore W4361962493C199901988 @default.
- W4361962493 hasConceptScore W4361962493C2776415932 @default.
- W4361962493 hasConceptScore W4361962493C2776438761 @default.
- W4361962493 hasConceptScore W4361962493C2777391703 @default.
- W4361962493 hasConceptScore W4361962493C2777609662 @default.
- W4361962493 hasConceptScore W4361962493C2778308172 @default.
- W4361962493 hasConceptScore W4361962493C2779306644 @default.
- W4361962493 hasConceptScore W4361962493C2779723316 @default.
- W4361962493 hasConceptScore W4361962493C502942594 @default.
- W4361962493 hasConceptScore W4361962493C55493867 @default.
- W4361962493 hasConceptScore W4361962493C62478195 @default.
- W4361962493 hasConceptScore W4361962493C71924100 @default.
- W4361962493 hasConceptScore W4361962493C75217442 @default.
- W4361962493 hasConceptScore W4361962493C86554907 @default.
- W4361962493 hasConceptScore W4361962493C86803240 @default.
- W4361962493 hasConceptScore W4361962493C95444343 @default.
- W4361962493 hasConceptScore W4361962493C96232424 @default.
- W4361962493 hasLocation W43619624931 @default.
- W4361962493 hasLocation W43619624932 @default.
- W4361962493 hasLocation W43619624933 @default.
- W4361962493 hasLocation W43619624934 @default.
- W4361962493 hasLocation W43619624935 @default.
- W4361962493 hasLocation W43619624936 @default.
- W4361962493 hasLocation W43619624937 @default.
- W4361962493 hasLocation W43619624938 @default.
- W4361962493 hasLocation W43619624939 @default.
- W4361962493 hasOpenAccess W4361962493 @default.
- W4361962493 hasPrimaryLocation W43619624931 @default.
- W4361962493 hasRelatedWork W2030981986 @default.
- W4361962493 hasRelatedWork W2044505245 @default.
- W4361962493 hasRelatedWork W2059056884 @default.
- W4361962493 hasRelatedWork W2068635845 @default.
- W4361962493 hasRelatedWork W2073315906 @default.
- W4361962493 hasRelatedWork W2088702072 @default.
- W4361962493 hasRelatedWork W2127124149 @default.
- W4361962493 hasRelatedWork W284870067 @default.
- W4361962493 hasRelatedWork W2986129865 @default.
- W4361962493 hasRelatedWork W4313594830 @default.
- W4361962493 isParatext "false" @default.
- W4361962493 isRetracted "false" @default.
- W4361962493 workType "article" @default.