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- W4362156419 abstract "<p>PDF file - 82K, 6A, The c-Src inhibitor blocked tumor necrosis factor (TNF) super family genes induced by E2. MCF-7:5C cells were treated with vehicle (0.1% DMSO), E2 (10-9 mol/L), 4-OHT (10-6 mol/L), E2 (10-9 mol/L) plus 4-OHT (10-6 mol/L), PP2 (5x10-6 mol/L), E2 (10-9 mol/L) plus PP2 (5x10-6 mol/L) respectively for 72 hours. Cells were harvested in TRIzol. LTA (TNF super family member 1) gene was detected by real-time PCR. P<0.001, ** compared with control. 6B, LTB (TNF super family member 3) gene was detected by real-time PCR. P<0.001, ** compared with control. 6C, TNFα activated apoptotic pathways in MCF-7:5C cells. MCF-7:5C cells were treated with vehicle (H2O) and TNFα (5ng/mL) for 24 hours. Cell lysates were harvested. Pro-apoptotic pathways PARP and Caspase-9 were examined by immunoblotting with primary antibodies. Immunoblotting for beta-actin was detected for loading control. 6D, TNFα inhibited MCF-7:5C cell growth. MCF-7:5C cells were treated with vehicle (H2O) and TNFα (5ng/mL) for 7 days. Cells were harvested and total DNA was determined using a DNA fluorescence quantitation kit. P<0.001, ** compared with control. All the data shown were representative of at least three separate experiments with similar results.</p>" @default.
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- W4362156419 date "2023-03-30" @default.
- W4362156419 modified "2023-09-29" @default.
- W4362156419 title "Supplementary Figure 6 from c-Src Modulates Estrogen-Induced Stress and Apoptosis in Estrogen-Deprived Breast Cancer Cells" @default.
- W4362156419 doi "https://doi.org/10.1158/0008-5472.22397571" @default.
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