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- W4362521912 abstract "Methods for the analysis of cell secretions at the single-cell level only provide semiquantitative endpoint readouts. Here we describe a microwell array for the real-time spatiotemporal monitoring of extracellular secretions from hundreds of single cells in parallel. The microwell array incorporates a gold substrate with arrays of nanometric holes functionalized with receptors for a specific analyte, and is illuminated with light spectrally overlapping with the device's spectrum of extraordinary optical transmission. Spectral shifts in surface plasmon resonance resulting from analyte-receptor bindings around a secreting cell are recorded by a camera as variations in the intensity of the transmitted light while machine-learning-assisted cell tracking eliminates the influence of cell movements. We used the microwell array to characterize the antibody-secretion profiles of hybridoma cells and of a rare subset of antibody-secreting cells sorted from human donor peripheral blood mononuclear cells. High-throughput measurements of spatiotemporal secretory profiles at the single-cell level will aid the study of the physiological mechanisms governing protein secretion." @default.
- W4362521912 created "2023-04-06" @default.
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- W4362521912 date "2023-04-03" @default.
- W4362521912 modified "2023-10-15" @default.
- W4362521912 title "High-throughput spatiotemporal monitoring of single-cell secretions via plasmonic microwell arrays" @default.
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- W4362521912 doi "https://doi.org/10.1038/s41551-023-01017-1" @default.
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