FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4362524498> ?p ?o ?g. }

• W4362524498 endingPage "692" @default.
• W4362524498 startingPage "692" @default.
• W4362524498 abstract "Abstract Introduction: Human papillomaviruses (HPVs) are responsible for about 25% of cancer cases worldwide. HPV-16 E7 antigen is a tumor-associated antigen (TAA) commonly expressed in HPV-induced tumors; however, it has low immunogenicity. The interaction of 4-1BBL with its receptor induces pleiotropic effects on innate, adaptive, and regulatory immunity and, if fused to TAAs in DNA vaccines, can improve the antitumor response; however, there is low transfection and antitumor efficiency. Oncolytic virotherapy is promising for antitumor gene therapy as it can be selectively replicated in tumor cells, inducing cell lysis, and furthermore, tumor cell debris can be taken in by immune cells to potentiate antitumor responses. Methods: The AdenoQuick 2.0 system was used to construct three OAds, expressing 1) SP-SA-E7-4-1BBL, 2) SA-E7-4-1BBL or 3) SP-SA-41BBL. The expression of transgenes was confirmed by western blot and subcellular localization of SP-SA-E7-4-1BBL was evaluated by immunofluorescence. The recombinant OAds-mediated cancer killing effect was evaluated by MTT assay and crystal violet staining, The recombinant OAds therapeutic efficacy was evaluated in a C57BL/6 mice bearing subcutaneous TC-1 lung tumor. Tumor growth and survival were monitored for a period of twenty-eight days. Results: In this study, we expressed the immunomodulatory molecule SA-4-1BBL fused to E7 on an oncolytic adenovirus (OAd) system. In vitro infection of TC-1 tumor cells and NIH-3T3 non-tumor cells with SA/E7/4-1BBL OAd demonstrated that only tumor cells are selectively destroyed. Moreover, protein expression is targeted to the endoplasmic reticulum in both cell lines when a signal peptide (SP) is added. Finally, in an HPV-induced cancer murine model, the therapeutic oncolytic activity of OAd can be detected, and this can be improved when fused to E7 and SP. Conclusions: In this study, we report the design of an oncolytic adenovirus expressing the SP/SA/E7/4-1BBL fusion gene, which is capable of infecting murine cancer and normal cells, and the expressed protein was able to be targeted to the endoplasmic reticulum. Most importantly, OAd induced a cell killing effect that is specific to cancer cells. Moreover, OAd treatment induced a potent antitumor effect in a mouse TC-1 cancer model. Citation Format: Jorge G. Gomez-Gutierrez, Rodolfo Garza-Morales, Alejandra G. Martinez-Perez, Jose J. Perez-Trujillo, Odila Saucedo-Cardenas, Maria J. Loera-Arias, Roberto Montes-de-Oca-Luna. OAd-mediated SA-4-1BBL fused to HPV-16 E7 elicits specific antitumor efficacy in syngeneic mouse lung cancer model [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 692." @default.
• W4362524498 created "2023-04-06" @default.
• W4362524498 creator A5053672834 @default.
• W4362524498 creator A5054146807 @default.
• W4362524498 creator A5063717244 @default.
• W4362524498 creator A5070547873 @default.
• W4362524498 creator A5077647701 @default.
• W4362524498 creator A5089899799 @default.
• W4362524498 creator A5090487181 @default.
• W4362524498 date "2023-04-04" @default.
• W4362524498 modified "2023-09-26" @default.
• W4362524498 title "Abstract 692: OAd-mediated SA-4-1BBL fused to HPV-16 E7 elicits specific antitumor efficacy in syngeneic mouse lung cancer model" @default.
• W4362524498 doi "https://doi.org/10.1158/1538-7445.am2023-692" @default.
• W4362524498 hasPublicationYear "2023" @default.
• W4362524498 type Work @default.
• W4362524498 citedByCount "0" @default.
• W4362524498 crossrefType "journal-article" @default.
• W4362524498 hasAuthorship W4362524498A5053672834 @default.
• W4362524498 hasAuthorship W4362524498A5054146807 @default.
• W4362524498 hasAuthorship W4362524498A5063717244 @default.
• W4362524498 hasAuthorship W4362524498A5070547873 @default.
• W4362524498 hasAuthorship W4362524498A5077647701 @default.
• W4362524498 hasAuthorship W4362524498A5089899799 @default.
• W4362524498 hasAuthorship W4362524498A5090487181 @default.
• W4362524498 hasConcept C121608353 @default.
• W4362524498 hasConcept C147483822 @default.
• W4362524498 hasConcept C153911025 @default.
• W4362524498 hasConcept C159047783 @default.
• W4362524498 hasConcept C203014093 @default.
• W4362524498 hasConcept C2776662205 @default.
• W4362524498 hasConcept C2777283488 @default.
• W4362524498 hasConcept C2777701055 @default.
• W4362524498 hasConcept C2780868878 @default.
• W4362524498 hasConcept C49744831 @default.
• W4362524498 hasConcept C502942594 @default.
• W4362524498 hasConcept C54355233 @default.
• W4362524498 hasConcept C82210918 @default.
• W4362524498 hasConcept C86803240 @default.
• W4362524498 hasConcept C8891405 @default.
• W4362524498 hasConcept C96232424 @default.
• W4362524498 hasConceptScore W4362524498C121608353 @default.
• W4362524498 hasConceptScore W4362524498C147483822 @default.
• W4362524498 hasConceptScore W4362524498C153911025 @default.
• W4362524498 hasConceptScore W4362524498C159047783 @default.
• W4362524498 hasConceptScore W4362524498C203014093 @default.
• W4362524498 hasConceptScore W4362524498C2776662205 @default.
• W4362524498 hasConceptScore W4362524498C2777283488 @default.
• W4362524498 hasConceptScore W4362524498C2777701055 @default.
• W4362524498 hasConceptScore W4362524498C2780868878 @default.
• W4362524498 hasConceptScore W4362524498C49744831 @default.
• W4362524498 hasConceptScore W4362524498C502942594 @default.
• W4362524498 hasConceptScore W4362524498C54355233 @default.
• W4362524498 hasConceptScore W4362524498C82210918 @default.
• W4362524498 hasConceptScore W4362524498C86803240 @default.
• W4362524498 hasConceptScore W4362524498C8891405 @default.
• W4362524498 hasConceptScore W4362524498C96232424 @default.
• W4362524498 hasIssue "7_Supplement" @default.
• W4362524498 hasLocation W43625244981 @default.
• W4362524498 hasOpenAccess W4362524498 @default.
• W4362524498 hasPrimaryLocation W43625244981 @default.
• W4362524498 hasRelatedWork W1861506798 @default.
• W4362524498 hasRelatedWork W1983046009 @default.
• W4362524498 hasRelatedWork W2777660397 @default.
• W4362524498 hasRelatedWork W2781731156 @default.
• W4362524498 hasRelatedWork W2953624074 @default.
• W4362524498 hasRelatedWork W3024592315 @default.
• W4362524498 hasRelatedWork W3127182391 @default.
• W4362524498 hasRelatedWork W3166852946 @default.
• W4362524498 hasRelatedWork W4283721225 @default.
• W4362524498 hasRelatedWork W4361208677 @default.
• W4362524498 hasVolume "83" @default.
• W4362524498 isParatext "false" @default.
• W4362524498 isRetracted "false" @default.
• W4362524498 workType "article" @default.