Matches in SemOpenAlex for { <https://semopenalex.org/work/W4362524913> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W4362524913 endingPage "1890" @default.
- W4362524913 startingPage "1890" @default.
- W4362524913 abstract "Abstract Tropomyosin kinase B (TrkB) plays an important role in survival, differentiation, and proliferation of neural cells. It belongs to the family of receptor tyrosine kinases and its specific ligands are NT-4/5 and BDNF. The (over)expression of TrkB and (over)activation of the BDNF/TrkB signaling pathway is implicated in various solid malignancies and associated with poor prognosis due to the role of TrkB in suppressing anoikis and promoting epithelial-mesenchymal transition. Here we present the development and characterization of novel T cell engagers targeting TrkB. A panel of 41 sequence unique antibodies were selected from human phage display libraries and B cells of TrkB-ECD immunized chicken. Following recombinant expression, the panel of antibodies was analyzed for off-rate, binding reactivity to plate-immobilized and cell-expressed TrkB, cross-reactivity to other tropomyosin kinase family members, and competition with BDNF. Based on their characteristics, two targeting arms were prioritized for the generation of T cell engagers using a standard bispecific knob-into-hole approach. Apart from a commercial CD3 targeting arm, three proprietary CD3 targeting arms were also analyzed. After successful production of the bispecifics , the molecules were validated to bind to plate-immobilized TrkB and CD3 separately and cell-associated TrkB and CD3 simultaneously. In addition, our TrkB T cell engagers were able to induce varying levels of T cell activation in vitro. Our data suggests that these TrkB/CD3 bispecific molecules could be interesting candidates for further development into immuno-oncology therapeutics. Citation Format: Jetta Bijlsma, Srinidhi Desikan, Timothy Miller, Allie Thom, Jennifer Bath, Ilse Roodink. Bispecific T cell engagers targeting TrkB [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 1890." @default.
- W4362524913 created "2023-04-06" @default.
- W4362524913 creator A5001439596 @default.
- W4362524913 creator A5017854775 @default.
- W4362524913 creator A5022326820 @default.
- W4362524913 creator A5029878974 @default.
- W4362524913 creator A5036370716 @default.
- W4362524913 creator A5063551543 @default.
- W4362524913 date "2023-04-04" @default.
- W4362524913 modified "2023-09-25" @default.
- W4362524913 title "Abstract 1890: Bispecific T cell engagers targeting TrkB" @default.
- W4362524913 doi "https://doi.org/10.1158/1538-7445.am2023-1890" @default.
- W4362524913 hasPublicationYear "2023" @default.
- W4362524913 type Work @default.
- W4362524913 citedByCount "0" @default.
- W4362524913 crossrefType "journal-article" @default.
- W4362524913 hasAuthorship W4362524913A5001439596 @default.
- W4362524913 hasAuthorship W4362524913A5017854775 @default.
- W4362524913 hasAuthorship W4362524913A5022326820 @default.
- W4362524913 hasAuthorship W4362524913A5029878974 @default.
- W4362524913 hasAuthorship W4362524913A5036370716 @default.
- W4362524913 hasAuthorship W4362524913A5063551543 @default.
- W4362524913 hasConcept C114684123 @default.
- W4362524913 hasConcept C147483822 @default.
- W4362524913 hasConcept C160539049 @default.
- W4362524913 hasConcept C167672396 @default.
- W4362524913 hasConcept C170493617 @default.
- W4362524913 hasConcept C171034665 @default.
- W4362524913 hasConcept C185592680 @default.
- W4362524913 hasConcept C203014093 @default.
- W4362524913 hasConcept C205875245 @default.
- W4362524913 hasConcept C42362537 @default.
- W4362524913 hasConcept C502942594 @default.
- W4362524913 hasConcept C55493867 @default.
- W4362524913 hasConcept C62478195 @default.
- W4362524913 hasConcept C6997183 @default.
- W4362524913 hasConcept C86803240 @default.
- W4362524913 hasConcept C92020748 @default.
- W4362524913 hasConcept C95444343 @default.
- W4362524913 hasConcept C97124661 @default.
- W4362524913 hasConceptScore W4362524913C114684123 @default.
- W4362524913 hasConceptScore W4362524913C147483822 @default.
- W4362524913 hasConceptScore W4362524913C160539049 @default.
- W4362524913 hasConceptScore W4362524913C167672396 @default.
- W4362524913 hasConceptScore W4362524913C170493617 @default.
- W4362524913 hasConceptScore W4362524913C171034665 @default.
- W4362524913 hasConceptScore W4362524913C185592680 @default.
- W4362524913 hasConceptScore W4362524913C203014093 @default.
- W4362524913 hasConceptScore W4362524913C205875245 @default.
- W4362524913 hasConceptScore W4362524913C42362537 @default.
- W4362524913 hasConceptScore W4362524913C502942594 @default.
- W4362524913 hasConceptScore W4362524913C55493867 @default.
- W4362524913 hasConceptScore W4362524913C62478195 @default.
- W4362524913 hasConceptScore W4362524913C6997183 @default.
- W4362524913 hasConceptScore W4362524913C86803240 @default.
- W4362524913 hasConceptScore W4362524913C92020748 @default.
- W4362524913 hasConceptScore W4362524913C95444343 @default.
- W4362524913 hasConceptScore W4362524913C97124661 @default.
- W4362524913 hasIssue "7_Supplement" @default.
- W4362524913 hasLocation W43625249131 @default.
- W4362524913 hasOpenAccess W4362524913 @default.
- W4362524913 hasPrimaryLocation W43625249131 @default.
- W4362524913 hasRelatedWork W1993354191 @default.
- W4362524913 hasRelatedWork W2348508725 @default.
- W4362524913 hasRelatedWork W2474117933 @default.
- W4362524913 hasRelatedWork W2912919627 @default.
- W4362524913 hasRelatedWork W2953752975 @default.
- W4362524913 hasRelatedWork W3026620415 @default.
- W4362524913 hasRelatedWork W3184395321 @default.
- W4362524913 hasRelatedWork W3204100626 @default.
- W4362524913 hasRelatedWork W3207218037 @default.
- W4362524913 hasRelatedWork W4311883962 @default.
- W4362524913 hasVolume "83" @default.
- W4362524913 isParatext "false" @default.
- W4362524913 isRetracted "false" @default.
- W4362524913 workType "article" @default.