FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4362561093> ?p ?o ?g. }

• W4362561093 abstract "<div>Abstract<p>Natural killer (NK) cells hold potential as a source of allogeneic cytotoxic effector cells for chimeric antigen receptor (CAR)-mediated therapies. Here, we explored the feasibility of transfecting CAR-encoding mRNA into primary NK cells and investigated how the intrinsic potential of discrete NK-cell subsets affects retargeting efficiency. After screening five second- and third-generation anti-CD19 CAR constructs with different signaling domains and spacer regions, a third-generation CAR with the CH2-domain removed was selected based on its expression and functional profiles. Kinetics experiments revealed that CAR expression was optimal after 3 days of IL15 stimulation prior to transfection, consistently achieving over 80% expression. CAR-engineered NK cells acquired increased degranulation toward CD19⁺ targets, and maintained their intrinsic degranulation response toward CD19⁻ K562 cells. The response of redirected NK-cell subsets against CD19⁺ targets was dependent on their intrinsic thresholds for activation determined through both differentiation and education by killer cell immunoglobulin-like receptors (KIR) and/or CD94/NKG2A binding to self HLA class I and HLA-E, respectively. Redirected primary NK cells were insensitive to inhibition through NKG2A/HLA-E interactions but remained sensitive to inhibition through KIR depending on the amount of HLA class I expressed on target cells. Adaptive NK cells, expressing NKG2C, CD57, and self-HLA–specific KIR(s), displayed superior ability to kill CD19⁺, HLA low, or mismatched tumor cells. These findings support the feasibility of primary allogeneic NK cells for CAR engineering and highlight a need to consider NK-cell diversity when optimizing efficacy of cancer immunotherapies based on CAR-expressing NK cells. <i>Cancer Immunol Res; 6(4); 467–80. ©2018 AACR</i>.</p></div>" @default.
• W4362561093 created "2023-04-06" @default.
• W4362561093 creator A5008236591 @default.
• W4362561093 creator A5021412783 @default.
• W4362561093 creator A5028004266 @default.
• W4362561093 creator A5035294592 @default.
• W4362561093 creator A5037775971 @default.
• W4362561093 creator A5042539600 @default.
• W4362561093 creator A5047523400 @default.
• W4362561093 creator A5049943311 @default.
• W4362561093 creator A5050488184 @default.
• W4362561093 creator A5056730527 @default.
• W4362561093 creator A5061606756 @default.
• W4362561093 creator A5061847588 @default.
• W4362561093 creator A5062742990 @default.
• W4362561093 creator A5066007525 @default.
• W4362561093 creator A5082007741 @default.
• W4362561093 creator A5083577911 @default.
• W4362561093 creator A5084144837 @default.
• W4362561093 date "2023-04-03" @default.
• W4362561093 modified "2023-09-24" @default.
• W4362561093 title "Data from Intrinsic Functional Potential of NK-Cell Subsets Constrains Retargeting Driven by Chimeric Antigen Receptors" @default.
• W4362561093 doi "https://doi.org/10.1158/2326-6066.c.6549229" @default.
• W4362561093 hasPublicationYear "2023" @default.
• W4362561093 type Work @default.
• W4362561093 citedByCount "0" @default.
• W4362561093 crossrefType "posted-content" @default.
• W4362561093 hasAuthorship W4362561093A5008236591 @default.
• W4362561093 hasAuthorship W4362561093A5021412783 @default.
• W4362561093 hasAuthorship W4362561093A5028004266 @default.
• W4362561093 hasAuthorship W4362561093A5035294592 @default.
• W4362561093 hasAuthorship W4362561093A5037775971 @default.
• W4362561093 hasAuthorship W4362561093A5042539600 @default.
• W4362561093 hasAuthorship W4362561093A5047523400 @default.
• W4362561093 hasAuthorship W4362561093A5049943311 @default.
• W4362561093 hasAuthorship W4362561093A5050488184 @default.
• W4362561093 hasAuthorship W4362561093A5056730527 @default.
• W4362561093 hasAuthorship W4362561093A5061606756 @default.
• W4362561093 hasAuthorship W4362561093A5061847588 @default.
• W4362561093 hasAuthorship W4362561093A5062742990 @default.
• W4362561093 hasAuthorship W4362561093A5066007525 @default.
• W4362561093 hasAuthorship W4362561093A5082007741 @default.
• W4362561093 hasAuthorship W4362561093A5083577911 @default.
• W4362561093 hasAuthorship W4362561093A5084144837 @default.
• W4362561093 hasConcept C147483822 @default.
• W4362561093 hasConcept C153911025 @default.
• W4362561093 hasConcept C154317977 @default.
• W4362561093 hasConcept C170493617 @default.
• W4362561093 hasConcept C185592680 @default.
• W4362561093 hasConcept C202751555 @default.
• W4362561093 hasConcept C203014093 @default.
• W4362561093 hasConcept C2776090121 @default.
• W4362561093 hasConcept C2778957590 @default.
• W4362561093 hasConcept C3875195 @default.
• W4362561093 hasConcept C49802076 @default.
• W4362561093 hasConcept C54009773 @default.
• W4362561093 hasConcept C54355233 @default.
• W4362561093 hasConcept C553184892 @default.
• W4362561093 hasConcept C55493867 @default.
• W4362561093 hasConcept C81885089 @default.
• W4362561093 hasConcept C86803240 @default.
• W4362561093 hasConcept C8891405 @default.
• W4362561093 hasConcept C95444343 @default.
• W4362561093 hasConceptScore W4362561093C147483822 @default.
• W4362561093 hasConceptScore W4362561093C153911025 @default.
• W4362561093 hasConceptScore W4362561093C154317977 @default.
• W4362561093 hasConceptScore W4362561093C170493617 @default.
• W4362561093 hasConceptScore W4362561093C185592680 @default.
• W4362561093 hasConceptScore W4362561093C202751555 @default.
• W4362561093 hasConceptScore W4362561093C203014093 @default.
• W4362561093 hasConceptScore W4362561093C2776090121 @default.
• W4362561093 hasConceptScore W4362561093C2778957590 @default.
• W4362561093 hasConceptScore W4362561093C3875195 @default.
• W4362561093 hasConceptScore W4362561093C49802076 @default.
• W4362561093 hasConceptScore W4362561093C54009773 @default.
• W4362561093 hasConceptScore W4362561093C54355233 @default.
• W4362561093 hasConceptScore W4362561093C553184892 @default.
• W4362561093 hasConceptScore W4362561093C55493867 @default.
• W4362561093 hasConceptScore W4362561093C81885089 @default.
• W4362561093 hasConceptScore W4362561093C86803240 @default.
• W4362561093 hasConceptScore W4362561093C8891405 @default.
• W4362561093 hasConceptScore W4362561093C95444343 @default.
• W4362561093 hasLocation W43625610931 @default.
• W4362561093 hasOpenAccess W4362561093 @default.
• W4362561093 hasPrimaryLocation W43625610931 @default.
• W4362561093 hasRelatedWork W2064357373 @default.
• W4362561093 hasRelatedWork W2369377256 @default.
• W4362561093 hasRelatedWork W2381657842 @default.
• W4362561093 hasRelatedWork W2401067645 @default.
• W4362561093 hasRelatedWork W2912741252 @default.
• W4362561093 hasRelatedWork W2990561844 @default.
• W4362561093 hasRelatedWork W3029927029 @default.
• W4362561093 hasRelatedWork W4281289906 @default.
• W4362561093 hasRelatedWork W4283333352 @default.
• W4362561093 hasRelatedWork W4318616170 @default.
• W4362561093 isParatext "false" @default.
• W4362561093 isRetracted "false" @default.
• W4362561093 workType "article" @default.