FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4362583885> ?p ?o ?g. }

• W4362583885 endingPage "225" @default.
• W4362583885 startingPage "215" @default.
• W4362583885 abstract "Single-layer centrifugation (SLC) with a low-density colloid is an efficient method for removing contaminating microorganisms from boar semen while recovering most spermatozoa from the original sample. This study tested the performance of this technique, using 50-ml tubes, by spiking commercial semen doses prepared without antibiotics with selected bacterial species followed by storage at 17 °C. The doses were spiked up to 102/ml CFU (colony forming units) of the bacteria Burkholderia ambifaria, Pseudomonas aeruginosa, and Staphylococcus simulans. The semen was processed by SLC (15 ml of sample and 15 ml of colloid) with the colloid Porcicoll at 20% (P20) and 30% (P30), with a spiked control (CTL) and an unspiked control (CTL0), analyzing microbiology and sperm quality on days 0, 3 and 7. SLC completely removed B. ambifaria and S. simulans, considerably reducing P. aeruginosa and overall contamination (especially P30, ∼104 CFU/ml of total contamination on day 7, median). Sperm viability was lower in P20 and P30 samples at day 0, with higher cytoplasmic ROS. Still, results were similar in all groups on day 3 and reversed on day 7, indicating a protective effect of SLC (possibly directly by removal of damaged sperm and indirectly because of lower bacterial contamination). Sperm chromatin was affected by the treatment (lower DNA fragmentation and chromatin decondensation) and storage (higher overall condensation on day 7 as per chromomycin A3 and monobromobimane staining). In conclusion, SLC with low-density colloids can remove most bacteria in a controlled contamination design while potentially improving sperm quality and long-term storage at practical temperatures." @default.
• W4362583885 created "2023-04-06" @default.
• W4362583885 creator A5010672984 @default.
• W4362583885 creator A5027115067 @default.
• W4362583885 creator A5035590997 @default.
• W4362583885 creator A5039670845 @default.
• W4362583885 creator A5056874444 @default.
• W4362583885 creator A5059397567 @default.
• W4362583885 date "2023-05-01" @default.
• W4362583885 modified "2023-09-30" @default.
• W4362583885 title "Low-density colloid centrifugation removes bacteria from boar semen doses after spiking with selected species" @default.
• W4362583885 cites W1903097060 @default.
• W4362583885 cites W1965675190 @default.
• W4362583885 cites W1990596691 @default.
• W4362583885 cites W1994660230 @default.
• W4362583885 cites W2000014577 @default.
• W4362583885 cites W2002058467 @default.
• W4362583885 cites W2011419271 @default.
• W4362583885 cites W2017405057 @default.
• W4362583885 cites W2028166632 @default.
• W4362583885 cites W2029725561 @default.
• W4362583885 cites W2035164460 @default.
• W4362583885 cites W2039680316 @default.
• W4362583885 cites W2045636690 @default.
• W4362583885 cites W2067675686 @default.
• W4362583885 cites W2077163742 @default.
• W4362583885 cites W2084507773 @default.
• W4362583885 cites W2092985083 @default.
• W4362583885 cites W2094532369 @default.
• W4362583885 cites W2102757463 @default.
• W4362583885 cites W2104691007 @default.
• W4362583885 cites W2108541919 @default.
• W4362583885 cites W2110080849 @default.
• W4362583885 cites W2117635909 @default.
• W4362583885 cites W2120373479 @default.
• W4362583885 cites W2136995979 @default.
• W4362583885 cites W2154993368 @default.
• W4362583885 cites W2239420337 @default.
• W4362583885 cites W2477980879 @default.
• W4362583885 cites W2536964940 @default.
• W4362583885 cites W2609803137 @default.
• W4362583885 cites W2621843916 @default.
• W4362583885 cites W2766771995 @default.
• W4362583885 cites W2801153474 @default.
• W4362583885 cites W2906143741 @default.
• W4362583885 cites W2981110398 @default.
• W4362583885 cites W2990492857 @default.
• W4362583885 cites W3003101556 @default.
• W4362583885 cites W3009040532 @default.
• W4362583885 cites W3014160303 @default.
• W4362583885 cites W3044546043 @default.
• W4362583885 cites W3121261840 @default.
• W4362583885 cites W3130777279 @default.
• W4362583885 cites W3139159150 @default.
• W4362583885 doi "https://doi.org/10.1016/j.rvsc.2023.03.024" @default.
• W4362583885 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37031470" @default.
• W4362583885 hasPublicationYear "2023" @default.
• W4362583885 type Work @default.
• W4362583885 citedByCount "0" @default.
• W4362583885 crossrefType "journal-article" @default.
• W4362583885 hasAuthorship W4362583885A5010672984 @default.
• W4362583885 hasAuthorship W4362583885A5027115067 @default.
• W4362583885 hasAuthorship W4362583885A5035590997 @default.
• W4362583885 hasAuthorship W4362583885A5039670845 @default.
• W4362583885 hasAuthorship W4362583885A5056874444 @default.
• W4362583885 hasAuthorship W4362583885A5059397567 @default.
• W4362583885 hasBestOaLocation W43625838851 @default.
• W4362583885 hasConcept C105702510 @default.
• W4362583885 hasConcept C112570922 @default.
• W4362583885 hasConcept C16685009 @default.
• W4362583885 hasConcept C175605896 @default.
• W4362583885 hasConcept C185592680 @default.
• W4362583885 hasConcept C18903297 @default.
• W4362583885 hasConcept C2777359717 @default.
• W4362583885 hasConcept C2778093475 @default.
• W4362583885 hasConcept C2781087480 @default.
• W4362583885 hasConcept C43617362 @default.
• W4362583885 hasConcept C523546767 @default.
• W4362583885 hasConcept C54355233 @default.
• W4362583885 hasConcept C59822182 @default.
• W4362583885 hasConcept C71924100 @default.
• W4362583885 hasConcept C7623868 @default.
• W4362583885 hasConcept C86803240 @default.
• W4362583885 hasConcept C89423630 @default.
• W4362583885 hasConceptScore W4362583885C105702510 @default.
• W4362583885 hasConceptScore W4362583885C112570922 @default.
• W4362583885 hasConceptScore W4362583885C16685009 @default.
• W4362583885 hasConceptScore W4362583885C175605896 @default.
• W4362583885 hasConceptScore W4362583885C185592680 @default.
• W4362583885 hasConceptScore W4362583885C18903297 @default.
• W4362583885 hasConceptScore W4362583885C2777359717 @default.
• W4362583885 hasConceptScore W4362583885C2778093475 @default.
• W4362583885 hasConceptScore W4362583885C2781087480 @default.
• W4362583885 hasConceptScore W4362583885C43617362 @default.
• W4362583885 hasConceptScore W4362583885C523546767 @default.
• W4362583885 hasConceptScore W4362583885C54355233 @default.
• W4362583885 hasConceptScore W4362583885C59822182 @default.
• W4362583885 hasConceptScore W4362583885C71924100 @default.

• next