FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4362657525> ?p ?o ?g. }

• W4362657525 abstract "Life requires ribonucleotide reduction for de novo synthesis of deoxyribonucleotides. As ribonucleotide reduction has on occasion been lost in parasites and endosymbionts, which are instead dependent on their host for deoxyribonucleotide synthesis, it should in principle be possible to knock this process out if growth media are supplemented with deoxyribonucleosides. We report the creation of a strain of Escherichia coli where all three ribonucleotide reductase operons have been deleted following introduction of a broad spectrum deoxyribonucleoside kinase from Mycoplasma mycoides. Our strain shows slowed but substantial growth in the presence of deoxyribonucleosides. Under limiting deoxyribonucleoside levels, we observe a distinctive filamentous cell morphology, where cells grow but do not appear to divide regularly. Finally, we examined whether our lines can adapt to limited supplies of deoxyribonucleosides, as might occur in the switch from de novo synthesis to dependence on host production during the evolution of parasitism or endosymbiosis. Over the course of an evolution experiment, we observe a 25-fold reduction in the minimum concentration of exogenous deoxyribonucleosides necessary for growth. Genome analysis reveals that several replicate lines carry mutations in deoB and cdd . deoB codes for phosphopentomutase, a key part of the deoxyriboaldolase pathway, which has been hypothesised as an alternative to ribonucleotide reduction for deoxyribonucleotide synthesis. Rather than complementing the loss of ribonucleotide reduction, our experiments reveal that mutations appear that reduce or eliminate the capacity for this pathway to catabolise deoxyribonucleotides, thus preventing their loss via central metabolism. Mutational inactivation of both deoB and cdd is also observed in a number of obligate intracellular bacteria that have lost ribonucleotide reduction. We conclude that our experiments recapitulate key evolutionary steps in the adaptation to life without ribonucleotide reduction." @default.
• W4362657525 created "2023-04-07" @default.
• W4362657525 creator A5006797577 @default.
• W4362657525 creator A5009335876 @default.
• W4362657525 creator A5014807381 @default.
• W4362657525 creator A5030514850 @default.
• W4362657525 creator A5032538132 @default.
• W4362657525 creator A5038913657 @default.
• W4362657525 creator A5040611230 @default.
• W4362657525 creator A5043581149 @default.
• W4362657525 creator A5044187288 @default.
• W4362657525 creator A5055956018 @default.
• W4362657525 creator A5072324097 @default.
• W4362657525 creator A5076001224 @default.
• W4362657525 date "2023-04-06" @default.
• W4362657525 modified "2023-10-18" @default.
• W4362657525 title "Characterisation of an Escherichia coli line that completely lacks ribonucleotide reduction yields insights into the evolution of parasitism and endosymbiosis" @default.
• W4362657525 cites W1519266993 @default.
• W4362657525 cites W1627863859 @default.
• W4362657525 cites W165403903 @default.
• W4362657525 cites W1781531090 @default.
• W4362657525 cites W1926303070 @default.
• W4362657525 cites W1966027779 @default.
• W4362657525 cites W1967600116 @default.
• W4362657525 cites W1973861730 @default.
• W4362657525 cites W1976722757 @default.
• W4362657525 cites W1978669792 @default.
• W4362657525 cites W1982410689 @default.
• W4362657525 cites W1982836127 @default.
• W4362657525 cites W1986375296 @default.
• W4362657525 cites W1987928401 @default.
• W4362657525 cites W1991194699 @default.
• W4362657525 cites W1993056701 @default.
• W4362657525 cites W2019385975 @default.
• W4362657525 cites W2024909602 @default.
• W4362657525 cites W2025454557 @default.
• W4362657525 cites W2034229618 @default.
• W4362657525 cites W2040042862 @default.
• W4362657525 cites W2044350546 @default.
• W4362657525 cites W2051643121 @default.
• W4362657525 cites W2052832659 @default.
• W4362657525 cites W2054431621 @default.
• W4362657525 cites W2055488120 @default.
• W4362657525 cites W2058078579 @default.
• W4362657525 cites W2058767645 @default.
• W4362657525 cites W2064306419 @default.
• W4362657525 cites W2068935039 @default.
• W4362657525 cites W2073258616 @default.
• W4362657525 cites W2082771430 @default.
• W4362657525 cites W2087245713 @default.
• W4362657525 cites W2093894897 @default.
• W4362657525 cites W2094625575 @default.
• W4362657525 cites W2099308633 @default.
• W4362657525 cites W2105199418 @default.
• W4362657525 cites W2112592262 @default.
• W4362657525 cites W2115622704 @default.
• W4362657525 cites W2123147993 @default.
• W4362657525 cites W2155582551 @default.
• W4362657525 cites W2159285197 @default.
• W4362657525 cites W2167456427 @default.
• W4362657525 cites W2170551349 @default.
• W4362657525 cites W2536789475 @default.
• W4362657525 cites W2581379592 @default.
• W4362657525 cites W2601546728 @default.
• W4362657525 cites W2796366830 @default.
• W4362657525 cites W2808177665 @default.
• W4362657525 cites W2890331430 @default.
• W4362657525 cites W2905802014 @default.
• W4362657525 cites W2952668961 @default.
• W4362657525 cites W2970842946 @default.
• W4362657525 cites W2978837790 @default.
• W4362657525 cites W2981337258 @default.
• W4362657525 cites W3048090444 @default.
• W4362657525 cites W3096988168 @default.
• W4362657525 cites W3152442338 @default.
• W4362657525 cites W4256321774 @default.
• W4362657525 cites W4283653993 @default.
• W4362657525 cites W4362657525 @default.
• W4362657525 doi "https://doi.org/10.7554/elife.83845" @default.
• W4362657525 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37022136" @default.
• W4362657525 hasPublicationYear "2023" @default.
• W4362657525 type Work @default.
• W4362657525 citedByCount "1" @default.
• W4362657525 countsByYear W43626575252023 @default.
• W4362657525 crossrefType "journal-article" @default.
• W4362657525 hasAuthorship W4362657525A5006797577 @default.
• W4362657525 hasAuthorship W4362657525A5009335876 @default.
• W4362657525 hasAuthorship W4362657525A5014807381 @default.
• W4362657525 hasAuthorship W4362657525A5030514850 @default.
• W4362657525 hasAuthorship W4362657525A5032538132 @default.
• W4362657525 hasAuthorship W4362657525A5038913657 @default.
• W4362657525 hasAuthorship W4362657525A5040611230 @default.
• W4362657525 hasAuthorship W4362657525A5043581149 @default.
• W4362657525 hasAuthorship W4362657525A5044187288 @default.
• W4362657525 hasAuthorship W4362657525A5055956018 @default.
• W4362657525 hasAuthorship W4362657525A5072324097 @default.
• W4362657525 hasAuthorship W4362657525A5076001224 @default.
• W4362657525 hasBestOaLocation W43626575251 @default.
• W4362657525 hasConcept C104292427 @default.
• W4362657525 hasConcept C104317684 @default.

• next