Matches in SemOpenAlex for { <https://semopenalex.org/work/W4362667780> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W4362667780 endingPage "3265" @default.
- W4362667780 startingPage "3265" @default.
- W4362667780 abstract "5-Amino-1-β-D-ribofuranosylimidazole-4-carboxamide 5'-monophosphate (ZMP) is a central intermediate in de novo purine nucleotide biosynthesis. Its nucleobase moiety, 5-aminoimidazole-4-carboxamide (Z-base), is considered an ambiguous base that can pair with any canonical base owing to the rotatable nature of its 5-carboxamide group. This idea of ambiguous base pairing due to free rotation of the carboxamide has been applied to designing mutagenic antiviral nucleosides, such as ribavirin and T-705. However, the ambiguous base-pairing ability of Z-base has not been elucidated, because the synthesis of Z-base-containing oligomers is problematic. Herein, we propose a practical method for the synthesis of Z-base-containing DNA oligomers based on the ring-opening reaction of an N1-dinitrophenylhypoxanthine (HxaDNP) base. Thermal denaturation studies of the resulting oligomers revealed that the Z-base behaves physiologically as an A-like nucleobase, preferentially forming pairs with T. We tested the behavior of Z-base-containing DNA oligomers in enzyme-catalyzed reactions: in single nucleotide insertion, Klenow fragment DNA polymerase recognized Z-base as an A-like analog and incorporated dTTP as a complementary nucleotide to Z-base in the DNA template; in PCR amplification, Taq DNA polymerase similarly incorporated dTTP as a complementary nucleotide to Z-base. Our findings will contribute to the development of new mutagenic antiviral nucleoside analogs." @default.
- W4362667780 created "2023-04-07" @default.
- W4362667780 creator A5000389670 @default.
- W4362667780 creator A5023275866 @default.
- W4362667780 creator A5026883944 @default.
- W4362667780 creator A5076999201 @default.
- W4362667780 date "2023-04-06" @default.
- W4362667780 modified "2023-10-18" @default.
- W4362667780 title "Synthesis and Behavior of DNA Oligomers Containing the Ambiguous Z-Nucleobase 5-Aminoimidazole-4-carboxamide" @default.
- W4362667780 cites W1515293536 @default.
- W4362667780 cites W1979371390 @default.
- W4362667780 cites W1992179301 @default.
- W4362667780 cites W1996080607 @default.
- W4362667780 cites W2003309895 @default.
- W4362667780 cites W2012051375 @default.
- W4362667780 cites W2013539008 @default.
- W4362667780 cites W2018928802 @default.
- W4362667780 cites W2027772101 @default.
- W4362667780 cites W2035234400 @default.
- W4362667780 cites W2089626253 @default.
- W4362667780 cites W2108367829 @default.
- W4362667780 cites W2114126506 @default.
- W4362667780 cites W2140084279 @default.
- W4362667780 cites W2147080317 @default.
- W4362667780 cites W2153568159 @default.
- W4362667780 cites W2164604555 @default.
- W4362667780 cites W2167623612 @default.
- W4362667780 cites W2785650761 @default.
- W4362667780 cites W2790956101 @default.
- W4362667780 cites W2883818819 @default.
- W4362667780 cites W3206791540 @default.
- W4362667780 cites W4224289728 @default.
- W4362667780 doi "https://doi.org/10.3390/molecules28073265" @default.
- W4362667780 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37050028" @default.
- W4362667780 hasPublicationYear "2023" @default.
- W4362667780 type Work @default.
- W4362667780 citedByCount "0" @default.
- W4362667780 crossrefType "journal-article" @default.
- W4362667780 hasAuthorship W4362667780A5000389670 @default.
- W4362667780 hasAuthorship W4362667780A5023275866 @default.
- W4362667780 hasAuthorship W4362667780A5026883944 @default.
- W4362667780 hasAuthorship W4362667780A5076999201 @default.
- W4362667780 hasBestOaLocation W43626677801 @default.
- W4362667780 hasConcept C104317684 @default.
- W4362667780 hasConcept C185581394 @default.
- W4362667780 hasConcept C185592680 @default.
- W4362667780 hasConcept C2756471 @default.
- W4362667780 hasConcept C2776543447 @default.
- W4362667780 hasConcept C2777501222 @default.
- W4362667780 hasConcept C2778327999 @default.
- W4362667780 hasConcept C512185932 @default.
- W4362667780 hasConcept C54415193 @default.
- W4362667780 hasConcept C552990157 @default.
- W4362667780 hasConcept C55493867 @default.
- W4362667780 hasConcept C61234307 @default.
- W4362667780 hasConcept C71240020 @default.
- W4362667780 hasConcept C82381507 @default.
- W4362667780 hasConcept C98892162 @default.
- W4362667780 hasConceptScore W4362667780C104317684 @default.
- W4362667780 hasConceptScore W4362667780C185581394 @default.
- W4362667780 hasConceptScore W4362667780C185592680 @default.
- W4362667780 hasConceptScore W4362667780C2756471 @default.
- W4362667780 hasConceptScore W4362667780C2776543447 @default.
- W4362667780 hasConceptScore W4362667780C2777501222 @default.
- W4362667780 hasConceptScore W4362667780C2778327999 @default.
- W4362667780 hasConceptScore W4362667780C512185932 @default.
- W4362667780 hasConceptScore W4362667780C54415193 @default.
- W4362667780 hasConceptScore W4362667780C552990157 @default.
- W4362667780 hasConceptScore W4362667780C55493867 @default.
- W4362667780 hasConceptScore W4362667780C61234307 @default.
- W4362667780 hasConceptScore W4362667780C71240020 @default.
- W4362667780 hasConceptScore W4362667780C82381507 @default.
- W4362667780 hasConceptScore W4362667780C98892162 @default.
- W4362667780 hasFunder F4320309785 @default.
- W4362667780 hasFunder F4320334764 @default.
- W4362667780 hasIssue "7" @default.
- W4362667780 hasLocation W43626677801 @default.
- W4362667780 hasLocation W43626677802 @default.
- W4362667780 hasLocation W43626677803 @default.
- W4362667780 hasOpenAccess W4362667780 @default.
- W4362667780 hasPrimaryLocation W43626677801 @default.
- W4362667780 hasRelatedWork W1980828861 @default.
- W4362667780 hasRelatedWork W2032528317 @default.
- W4362667780 hasRelatedWork W2058979516 @default.
- W4362667780 hasRelatedWork W2069352337 @default.
- W4362667780 hasRelatedWork W2086476153 @default.
- W4362667780 hasRelatedWork W2087714034 @default.
- W4362667780 hasRelatedWork W2121283001 @default.
- W4362667780 hasRelatedWork W2160303323 @default.
- W4362667780 hasRelatedWork W2341512235 @default.
- W4362667780 hasRelatedWork W4362667780 @default.
- W4362667780 hasVolume "28" @default.
- W4362667780 isParatext "false" @default.
- W4362667780 isRetracted "false" @default.
- W4362667780 workType "article" @default.