FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4362671504> ?p ?o ?g. }

• W4362671504 abstract "Artificial phase-separating (PS) peptides can be used in various applications such as microreactors and drug delivery; however, the design of artificial PS peptides remains a challenge. This can be attributed to the limitation of PS-relevant residues that drive phase separation by interactions of their pairs in short peptides and the difficulty in the design involving interaction with target PS proteins. In this study, we propose a rational method to design artificial PS peptides that satisfy the requirements of liquid droplet formation and co-phase separation with target PS proteins based on the target PS protein sequence. As a proof of concept, we designed five artificial peptides from the model PS protein p53 using this method and confirmed their PS properties using differential interference contrast and fluorescence microscopy. Single-molecule fluorescent tracking demonstrated rapid diffusion of the designed peptides in their droplets compared to that of p53 in p53 droplets. In addition, size-dependent uptake of p53 oligomers was observed in the designed peptide droplets. Large oligomers were excluded from the droplet voids and localized on the droplet surface. The uptake of high-order p53 oligomers into the droplets was enhanced by the elongated linker of the designed peptides. Furthermore, we found that the designed peptide droplets recruited p53 to suppress gel-like aggregate formation. Finally, we discuss aspects that were crucial in the successful design of the artificial PS peptides." @default.
• W4362671504 created "2023-04-07" @default.
• W4362671504 creator A5042864770 @default.
• W4362671504 creator A5060962197 @default.
• W4362671504 creator A5066254596 @default.
• W4362671504 creator A5074683056 @default.
• W4362671504 creator A5074953114 @default.
• W4362671504 creator A5080589417 @default.
• W4362671504 creator A5088515417 @default.
• W4362671504 date "2023-04-06" @default.
• W4362671504 modified "2023-09-26" @default.
• W4362671504 title "Rational design of phase separating peptides based on phase separating protein sequence of p53" @default.
• W4362671504 cites W1177958319 @default.
• W4362671504 cites W1998730965 @default.
• W4362671504 cites W2055043387 @default.
• W4362671504 cites W2111796925 @default.
• W4362671504 cites W2168639701 @default.
• W4362671504 cites W2171546617 @default.
• W4362671504 cites W2290455074 @default.
• W4362671504 cites W2408322376 @default.
• W4362671504 cites W2519649749 @default.
• W4362671504 cites W2531940417 @default.
• W4362671504 cites W2590568317 @default.
• W4362671504 cites W2623606714 @default.
• W4362671504 cites W2713865210 @default.
• W4362671504 cites W2755105920 @default.
• W4362671504 cites W2756821926 @default.
• W4362671504 cites W2766423371 @default.
• W4362671504 cites W2767977912 @default.
• W4362671504 cites W2782519992 @default.
• W4362671504 cites W2785420592 @default.
• W4362671504 cites W2786469974 @default.
• W4362671504 cites W2786589174 @default.
• W4362671504 cites W2795571682 @default.
• W4362671504 cites W2797019005 @default.
• W4362671504 cites W2802667264 @default.
• W4362671504 cites W2810866092 @default.
• W4362671504 cites W2884216445 @default.
• W4362671504 cites W2903264039 @default.
• W4362671504 cites W2943245415 @default.
• W4362671504 cites W2953933961 @default.
• W4362671504 cites W2954453835 @default.
• W4362671504 cites W2955037487 @default.
• W4362671504 cites W2969881735 @default.
• W4362671504 cites W2980466050 @default.
• W4362671504 cites W2991346024 @default.
• W4362671504 cites W2999733017 @default.
• W4362671504 cites W3005177895 @default.
• W4362671504 cites W3076447602 @default.
• W4362671504 cites W3090700963 @default.
• W4362671504 cites W3092787915 @default.
• W4362671504 cites W3108439960 @default.
• W4362671504 cites W3112123143 @default.
• W4362671504 cites W3131479422 @default.
• W4362671504 cites W3133422315 @default.
• W4362671504 cites W3136460629 @default.
• W4362671504 cites W3153026017 @default.
• W4362671504 cites W3173691267 @default.
• W4362671504 cites W3192300249 @default.
• W4362671504 cites W3203395139 @default.
• W4362671504 cites W3205791798 @default.
• W4362671504 cites W4220848114 @default.
• W4362671504 cites W4225284472 @default.
• W4362671504 cites W4229014893 @default.
• W4362671504 cites W4236805864 @default.
• W4362671504 cites W4281722976 @default.
• W4362671504 cites W4291145178 @default.
• W4362671504 doi "https://doi.org/10.1038/s41598-023-32632-2" @default.
• W4362671504 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37024567" @default.
• W4362671504 hasPublicationYear "2023" @default.
• W4362671504 type Work @default.
• W4362671504 citedByCount "0" @default.
• W4362671504 crossrefType "journal-article" @default.
• W4362671504 hasAuthorship W4362671504A5042864770 @default.
• W4362671504 hasAuthorship W4362671504A5060962197 @default.
• W4362671504 hasAuthorship W4362671504A5066254596 @default.
• W4362671504 hasAuthorship W4362671504A5074683056 @default.
• W4362671504 hasAuthorship W4362671504A5074953114 @default.
• W4362671504 hasAuthorship W4362671504A5080589417 @default.
• W4362671504 hasAuthorship W4362671504A5088515417 @default.
• W4362671504 hasBestOaLocation W43626715041 @default.
• W4362671504 hasConcept C111919701 @default.
• W4362671504 hasConcept C121332964 @default.
• W4362671504 hasConcept C12554922 @default.
• W4362671504 hasConcept C171250308 @default.
• W4362671504 hasConcept C178790620 @default.
• W4362671504 hasConcept C185592680 @default.
• W4362671504 hasConcept C192071366 @default.
• W4362671504 hasConcept C192562407 @default.
• W4362671504 hasConcept C21951064 @default.
• W4362671504 hasConcept C2778112365 @default.
• W4362671504 hasConcept C2779281246 @default.
• W4362671504 hasConcept C2780557392 @default.
• W4362671504 hasConcept C41008148 @default.
• W4362671504 hasConcept C44280652 @default.
• W4362671504 hasConcept C53105671 @default.
• W4362671504 hasConcept C55493867 @default.
• W4362671504 hasConcept C62520636 @default.
• W4362671504 hasConcept C69357855 @default.
• W4362671504 hasConcept C8673954 @default.

• next