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• W4362694156 abstract "Abstract Histone lysine methyltransferases (HKMTs) perform vital roles in cellular life by controlling gene expression programs through the posttranslational modification of histone tails. Many of them are intimately involved in the development of different diseases, including several cancers, understanding the molecular mechanisms that control their target recognition and activity is therefore vital for the treatment and prevention of such conditions. RNA binding has been shown to be an important regulatory factor in the function of several HKMTs, such as the yeast Set1 and the human Ezh2. Moreover, many HKMTs are capable of RNA binding in the absence of a canonical RNA binding domain. Here, we explored the RNA binding capacity of KMT2D, one of the major H3K4 monomethyl transferases in enhancers, using RNA immunoprecipitation sequencing. We identified a broad range of coding and non-coding RNAs associated with KMT2D and confirmed their binding through RNA immunoprecipitation and quantitative PCR. We also showed that a separated RNA binding region within KMT2D is capable of binding a similar RNA pool, but differences in the binding specificity indicate the existence of other regulatory elements in the sequence of KMT2D. Analysis of the bound mRNAs revealed that KMT2D preferentially binds contranscriptionally to the mRNAs of the genes under its control, while also interacting with super enhancer- and splicing-related non-coding RNAs. These observations, together with the nuclear colocalization of KMT2D with differentially phosphorylated forms of RNA Polymerase II suggest a so far unexplored role of KMT2D in the RNA processing of the nascent transcripts. Overexpression of the RNA binding region with the polyQ region known to drive phase separation of KMT2D revealed that while RNA binding and phase separation are both necessary and sufficient to target KMT2D to super enhancer condensates, a fully functional protein is indispensable for the proper assembly and functioning of the transcription factory." @default.
• W4362694156 created "2023-04-09" @default.
• W4362694156 creator A5014517086 @default.
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• W4362694156 creator A5071707864 @default.
• W4362694156 date "2023-04-07" @default.
• W4362694156 modified "2023-10-14" @default.
• W4362694156 title "KMT2D preferentially binds mRNAs of the genes it regulates, suggesting a role in RNA processing" @default.
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• W4362694156 doi "https://doi.org/10.21203/rs.3.rs-2746610/v1" @default.
• W4362694156 hasPublicationYear "2023" @default.
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