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- W4362700877 abstract "Abstract When a healthy, full-size vagina is absent due to a disorder, various neovagina creation methods are available. Sometimes dilation or stretching of the vagina cavity is sufficient, but generally intestinal or dermal graft tissue is required. However, different inherent tissue properties cause complications. Therefore, when a body part is lost, it should be replaced by a similar material. The use of organ-specific acellular vaginal tissue carries great potential, as the similar architecture and matrix composition make it fit for vagina regeneration. We developed an optimized decellularization protocol for human vaginal tissue and determined suitability as tissue-mimicking scaffold for vagina reconstruction. Histological examination confirmed the preservation of structural features and minimal cellular residue was seen during fluorescence microscopy, DNA and RNA quantification and fragment-length examination. Biomechanical testing showed decreased (P<0,05) strain at rupture (23%), tensile stress (55%) and elastic modulus (68%) after decellularization. Fluorescence microscopy revealed preserved Fibronectin-I/II/III and Laminin-I/II, while Collagen-I and Ficolin-2B were decreased but mostly retained. The absence of cellular residue, minimally altered biomechanical ECM properties and mostly preserved structural proteins, appear to make our decellularized human vaginal matrix a suitable tissue-mimicking scaffold for vagina transplantation when tissue survival through vascularization and innervation are accomplished in the future." @default.
- W4362700877 created "2023-04-09" @default.
- W4362700877 creator A5016526783 @default.
- W4362700877 creator A5021571226 @default.
- W4362700877 creator A5035607142 @default.
- W4362700877 creator A5042829338 @default.
- W4362700877 creator A5046977837 @default.
- W4362700877 creator A5056100319 @default.
- W4362700877 creator A5058070815 @default.
- W4362700877 creator A5059260142 @default.
- W4362700877 date "2023-04-06" @default.
- W4362700877 modified "2023-09-30" @default.
- W4362700877 title "Vagina reconstruction by decellularization of healthy human vaginal tissue" @default.
- W4362700877 cites W1512508466 @default.
- W4362700877 cites W1552661585 @default.
- W4362700877 cites W1564140484 @default.
- W4362700877 cites W1588909129 @default.
- W4362700877 cites W1592869029 @default.
- W4362700877 cites W1789148173 @default.
- W4362700877 cites W1909368374 @default.
- W4362700877 cites W1910864111 @default.
- W4362700877 cites W1953018149 @default.
- W4362700877 cites W1963670703 @default.
- W4362700877 cites W1964313111 @default.
- W4362700877 cites W1964569936 @default.
- W4362700877 cites W1966162083 @default.
- W4362700877 cites W1966729619 @default.
- W4362700877 cites W1967171619 @default.
- W4362700877 cites W1968271825 @default.
- W4362700877 cites W1972461014 @default.
- W4362700877 cites W1973602411 @default.
- W4362700877 cites W1975158756 @default.
- W4362700877 cites W1983772892 @default.
- W4362700877 cites W1987602789 @default.
- W4362700877 cites W1990142689 @default.
- W4362700877 cites W1991653488 @default.
- W4362700877 cites W1993163373 @default.
- W4362700877 cites W1995181257 @default.
- W4362700877 cites W1999579326 @default.
- W4362700877 cites W2001766290 @default.
- W4362700877 cites W2004822806 @default.
- W4362700877 cites W2010500001 @default.
- W4362700877 cites W2015023711 @default.
- W4362700877 cites W2015054568 @default.
- W4362700877 cites W2017567121 @default.
- W4362700877 cites W2027155599 @default.
- W4362700877 cites W2032032998 @default.
- W4362700877 cites W2034887920 @default.
- W4362700877 cites W2040898123 @default.
- W4362700877 cites W2041488558 @default.
- W4362700877 cites W2043509357 @default.
- W4362700877 cites W2059127559 @default.
- W4362700877 cites W2059486645 @default.
- W4362700877 cites W2061673735 @default.
- W4362700877 cites W2062007028 @default.
- W4362700877 cites W2064057495 @default.
- W4362700877 cites W2065343560 @default.
- W4362700877 cites W2065601344 @default.
- W4362700877 cites W2070667891 @default.
- W4362700877 cites W2070781752 @default.
- W4362700877 cites W2073227223 @default.
- W4362700877 cites W2073334588 @default.
- W4362700877 cites W2074011913 @default.
- W4362700877 cites W2081477525 @default.
- W4362700877 cites W2087161380 @default.
- W4362700877 cites W2104223523 @default.
- W4362700877 cites W2112507290 @default.
- W4362700877 cites W2116307340 @default.
- W4362700877 cites W2124273428 @default.
- W4362700877 cites W2124346745 @default.
- W4362700877 cites W2125516407 @default.
- W4362700877 cites W2127296180 @default.
- W4362700877 cites W2135973319 @default.
- W4362700877 cites W214395474 @default.
- W4362700877 cites W2148534563 @default.
- W4362700877 cites W2150685231 @default.
- W4362700877 cites W2152056221 @default.
- W4362700877 cites W2160023115 @default.
- W4362700877 cites W2160882127 @default.
- W4362700877 cites W2167949866 @default.
- W4362700877 cites W2168539681 @default.
- W4362700877 cites W2169097541 @default.
- W4362700877 cites W2170498291 @default.
- W4362700877 cites W2172793319 @default.
- W4362700877 cites W2178577279 @default.
- W4362700877 cites W2188039324 @default.
- W4362700877 cites W2278493172 @default.
- W4362700877 cites W2292205094 @default.
- W4362700877 cites W2301088222 @default.
- W4362700877 cites W2317797579 @default.
- W4362700877 cites W2325796265 @default.
- W4362700877 cites W2384752277 @default.
- W4362700877 cites W2399599124 @default.
- W4362700877 cites W2400109791 @default.
- W4362700877 cites W2477334841 @default.
- W4362700877 cites W2497317386 @default.
- W4362700877 cites W2515453911 @default.
- W4362700877 cites W2526158453 @default.
- W4362700877 cites W2543579241 @default.
- W4362700877 cites W2549976854 @default.