Matches in SemOpenAlex for { <https://semopenalex.org/work/W4362704177> ?p ?o ?g. }
- W4362704177 abstract "Abstract In vitro maturation of porcine oocytes is characterized by asynchronous cytoplasmic and nuclear maturation, leading to less competent oocytes supporting embryo development. The purpose of this study was to evaluate the combined effect of rolipram and cilostamide as cyclic Adenine monophosphate (cAMP) modulators to find the maximum cAMP levels that temporarily arrest meiosis. We determined the optimal time to maintain functional gap junction communication during pre-in vitro maturation to be four hours. Oocyte competence was evaluated by the level of glutathione, reactive oxygen species, meiotic progression, and gene expression. We evaluated embryonic developmental competence after parthenogenetic activation and somatic cell nuclear transfer. The combined treatment group showed significantly higher glutathione and lower reactive oxygen species levels and a higher maturation rate than the control and single treatment groups. Cleavage and blastocyst formation rates in parthenogenetic activation and somatic cell nuclear transfer embryos were higher in two-phase in vitro maturation than in the other groups. The relative levels of BMP15 and GDF9 expression were increased in two-phase in vitro maturation. Somatic cell nuclear transfer blastocysts from two-phase in vitro maturation oocytes showed a lower level of expression of apoptotic genes than the control, indicating better pre-implantation developmental competence. The combination of rolipram and cilostamide resulted in optimal synchrony of cytoplasmic and nuclear maturation in porcine in vitro matured oocytes and there by enhanced the developmental competence of pre-implantation embryos." @default.
- W4362704177 created "2023-04-09" @default.
- W4362704177 creator A5001967366 @default.
- W4362704177 creator A5014682862 @default.
- W4362704177 creator A5016666849 @default.
- W4362704177 creator A5017008084 @default.
- W4362704177 creator A5020531875 @default.
- W4362704177 creator A5025178482 @default.
- W4362704177 creator A5029656238 @default.
- W4362704177 creator A5032813464 @default.
- W4362704177 creator A5033427257 @default.
- W4362704177 creator A5033960616 @default.
- W4362704177 creator A5054483572 @default.
- W4362704177 creator A5079279871 @default.
- W4362704177 date "2023-04-07" @default.
- W4362704177 modified "2023-10-14" @default.
- W4362704177 title "The combination of rolipram and cilostamide improved the developmental competence of cloned porcine embryos" @default.
- W4362704177 cites W1484481964 @default.
- W4362704177 cites W1567416057 @default.
- W4362704177 cites W1916885428 @default.
- W4362704177 cites W1963495485 @default.
- W4362704177 cites W1963832918 @default.
- W4362704177 cites W1964881942 @default.
- W4362704177 cites W1965593095 @default.
- W4362704177 cites W1972501524 @default.
- W4362704177 cites W1973690432 @default.
- W4362704177 cites W1978592193 @default.
- W4362704177 cites W1978687637 @default.
- W4362704177 cites W1980855623 @default.
- W4362704177 cites W1985816197 @default.
- W4362704177 cites W1987053150 @default.
- W4362704177 cites W1989696051 @default.
- W4362704177 cites W1992816621 @default.
- W4362704177 cites W2000039835 @default.
- W4362704177 cites W2002488124 @default.
- W4362704177 cites W2006460317 @default.
- W4362704177 cites W2010348828 @default.
- W4362704177 cites W2011844493 @default.
- W4362704177 cites W2017217180 @default.
- W4362704177 cites W2025336888 @default.
- W4362704177 cites W2028044019 @default.
- W4362704177 cites W2030061481 @default.
- W4362704177 cites W2030621462 @default.
- W4362704177 cites W2031661391 @default.
- W4362704177 cites W2038632075 @default.
- W4362704177 cites W2040392785 @default.
- W4362704177 cites W2041579008 @default.
- W4362704177 cites W2050014442 @default.
- W4362704177 cites W2054167608 @default.
- W4362704177 cites W2055950362 @default.
- W4362704177 cites W2057403288 @default.
- W4362704177 cites W2058954610 @default.
- W4362704177 cites W2071060609 @default.
- W4362704177 cites W2071423215 @default.
- W4362704177 cites W2072318929 @default.
- W4362704177 cites W2074582018 @default.
- W4362704177 cites W2083682485 @default.
- W4362704177 cites W2085684986 @default.
- W4362704177 cites W2092094963 @default.
- W4362704177 cites W2099096389 @default.
- W4362704177 cites W2102560485 @default.
- W4362704177 cites W2106654447 @default.
- W4362704177 cites W2107759031 @default.
- W4362704177 cites W2114504796 @default.
- W4362704177 cites W2117116006 @default.
- W4362704177 cites W2126364346 @default.
- W4362704177 cites W2128801396 @default.
- W4362704177 cites W2130047944 @default.
- W4362704177 cites W2131314599 @default.
- W4362704177 cites W2136201122 @default.
- W4362704177 cites W2144573151 @default.
- W4362704177 cites W2144883045 @default.
- W4362704177 cites W2147940687 @default.
- W4362704177 cites W2149237119 @default.
- W4362704177 cites W2150679731 @default.
- W4362704177 cites W2155323291 @default.
- W4362704177 cites W2156992491 @default.
- W4362704177 cites W2163649189 @default.
- W4362704177 cites W2170530908 @default.
- W4362704177 cites W2171161531 @default.
- W4362704177 cites W2174846695 @default.
- W4362704177 cites W2294744052 @default.
- W4362704177 cites W2316678914 @default.
- W4362704177 cites W2461066519 @default.
- W4362704177 cites W2473572099 @default.
- W4362704177 cites W2485681905 @default.
- W4362704177 cites W2580767461 @default.
- W4362704177 cites W2620885215 @default.
- W4362704177 cites W2622160583 @default.
- W4362704177 cites W2754792405 @default.
- W4362704177 cites W2770284256 @default.
- W4362704177 cites W2790644375 @default.
- W4362704177 cites W2803502119 @default.
- W4362704177 cites W2811289742 @default.
- W4362704177 cites W2899544616 @default.
- W4362704177 cites W2915005347 @default.
- W4362704177 cites W2946366105 @default.
- W4362704177 cites W2949432513 @default.
- W4362704177 cites W2969755598 @default.
- W4362704177 cites W2974464589 @default.