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- W4362704921 abstract "Diabetes increases the risk of cognitive decline and impairs brain function. Whether or not this relationship between high glucose and cognitive deficits is causal remains elusive. Moreover, whether these deficits are mediated by an increase in glucose levels in cerebrospinal fluid (CSF) and/or blood is also unclear. There are very few studies investigating the direct effects of high CSF glucose levels on central nervous system (CNS) function, especially on learning and memory, since current diabetes models are not sufficiently developed to address such research questions. This article describes a method to chronically increase CSF glucose levels for 4 weeks by continuously infusing glucose into the lateral ventricle using osmotic minipumps in mice. The protocol was validated by measuring glucose levels in CSF. This protocol increased CSF glucose levels to ~328 mg/dL after infusion of a 50% glucose solution at a 0.25 µL/h flow rate, compared to a CSF glucose concentration of ~56 mg/dL in mice that received artificial cerebrospinal fluid (aCSF). Furthermore, this protocol did not affect blood glucose levels. Therefore, this method can be used to determine the direct effects of high CSF glucose on brain function or a specific neural pathway independently of changes in blood glucose levels. Overall, the approach described here will facilitate the development of animal models for testing the role of high CSF glucose in mediating features of Alzheimer's disease and/or other neurodegenerative disorders associated with diabetes." @default.
- W4362704921 created "2023-04-09" @default.
- W4362704921 creator A5002504323 @default.
- W4362704921 creator A5028698153 @default.
- W4362704921 date "2023-04-07" @default.
- W4362704921 modified "2023-10-16" @default.
- W4362704921 title "Osmotic Minipump Implantation for Increasing Glucose Concentration in Mouse Cerebrospinal Fluid" @default.
- W4362704921 doi "https://doi.org/10.3791/65169" @default.
- W4362704921 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37092835" @default.
- W4362704921 hasPublicationYear "2023" @default.
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