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• W4362720544 abstract "Proteomic analysis of human plasma by LC-ESI-MS/MS has discovered a limited number of new cellular protein biomarkers that may be confirmed by independent biochemical methods. Analysis of COVID-19 plasma has indicated the re-purposing of known biomarkers that might be used as prognostic markers of COVID-19 infection. However, multiple molecular approaches have previously indicated that the SARS-COV2 infection cycle is linked to the biology of mitochondria and that the response to infections may involve the action of heme containing oxidative enzymes.Human plasma from COVID-19 and ICU-ARDS was analyzed by classical analytical biochemistry techniques and classical frequency-based statistical approaches to look for prognostic markers of severe COVID-19 lung damage. Plasma proteins from COVID-19 and ICU-ARDS were identified and enumerated versus the controls of normal human plasma (NHP) by LC-ESI-MS/MS. The observation frequency of proteins detected in COVID-19 and ICU-ARDS patients were compared to normal human plasma, alongside random and noise MS/MS spectra controls, using the Chi Square (χ2) distribution.PCR showed the presence of MT-ND1 DNA in the plasma of COVID-19, ICU-ARDS, as well as normal human plasma. Mitochondrial proteins such as MRPL, L2HGDH, ATP, CYB, CYTB, CYP, NDUF and others, were increased in COVID-19 and ICU-ARDS plasma. The apparent activity of the cytochrome components were tested alongside NHP by dot blotting on PVDF against a purified cytochrome c standard preparation for H2O2 dependent reaction with luminol as measured by enhanced chemiluminescence (ECL) that showed increased activity in COVID-19 and ICU-ARDS patients.The results from PCR, LC-ESI-MS/MS of tryptic peptides, and cytochrome ECL assays confirmed that mitochondrial components were present in the plasma, in agreement with the established central role of the mitochondria in SARS-COV-2 biology. The cytochrome activity assay showed that there was the equivalent of at least nanogram amounts of cytochrome(s) in the plasma sample that should be clearly detectable by LC-ESI-MS/MS. The release of the luminol oxidase activity from cells into plasma forms the basis of a simple and rapid test for the severity of cell damage and lung injury in COVID-19 infection and ICU-ARDS." @default.
• W4362720544 created "2023-04-09" @default.
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• W4362720544 date "2023-04-08" @default.
• W4362720544 modified "2023-10-14" @default.
• W4362720544 title "Mitochondria and cytochrome components released into the plasma of severe COVID-19 and ICU acute respiratory distress syndrome patients" @default.
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• W4362720544 cites W1998741437 @default.
• W4362720544 cites W2002439304 @default.
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• W4362720544 cites W2030997256 @default.
• W4362720544 cites W2033492963 @default.
• W4362720544 cites W2033571031 @default.
• W4362720544 cites W2043155515 @default.
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• W4362720544 cites W2151282768 @default.
• W4362720544 cites W2153658751 @default.
• W4362720544 cites W2157144491 @default.
• W4362720544 cites W2566102821 @default.
• W4362720544 cites W2581455066 @default.
• W4362720544 cites W2583541072 @default.
• W4362720544 cites W2624338235 @default.
• W4362720544 cites W2765472463 @default.
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• W4362720544 cites W3131820551 @default.
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• W4362720544 doi "https://doi.org/10.1186/s12014-023-09394-0" @default.
• W4362720544 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37031181" @default.
• W4362720544 hasPublicationYear "2023" @default.
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