Matches in SemOpenAlex for { <https://semopenalex.org/work/W4362721829> ?p ?o ?g. }
- W4362721829 endingPage "106533" @default.
- W4362721829 startingPage "106533" @default.
- W4362721829 abstract "Penicillin G acylase (PGA) is a key biocatalyst for the enzymatic production of β-lactam antibiotics, which can not only catalyze the synthesis of β-lactam antibiotics but also catalyze the hydrolysis of the products to prepare semi-synthetic antibiotic intermediates. However, the high hydrolysis and low synthesis activities of natural PGAs severely hinder their industrial application. In this study, a combinatorial directed evolution strategy was employed to obtain new PGAs with outstanding performances. The best mutant βF24G/βW154G was obtained from the PGA of Achromobacter sp., which exhibited approximately a 129.62-fold and a 52.55-fold increase in specific activity and synthesis/hydrolysis ratio, respectively, compared to the wild-type AsPGA. Thereafter, this mutant was used to synthesize amoxicillin, cefadroxil, and ampicillin; all conversions > 99% were accomplished in 90–135 min with almost no secondary hydrolysis byproducts produced in the reaction. Molecular dynamics simulation and substrate pocket calculation revealed that substitution of the smallest glycine residue at βF24 and βW154 expanded the binding pocket, thereby facilitating the entry and release of substrates and products. Therefore, this novel mutant is a promising catalyst for the large-scale production of β-lactam antibiotics." @default.
- W4362721829 created "2023-04-09" @default.
- W4362721829 creator A5011549562 @default.
- W4362721829 creator A5019176281 @default.
- W4362721829 creator A5024015895 @default.
- W4362721829 creator A5024415495 @default.
- W4362721829 creator A5050302262 @default.
- W4362721829 creator A5070804547 @default.
- W4362721829 creator A5075363504 @default.
- W4362721829 creator A5078323046 @default.
- W4362721829 creator A5082156574 @default.
- W4362721829 date "2023-07-01" @default.
- W4362721829 modified "2023-10-18" @default.
- W4362721829 title "Enlarging the substrate binding pocket of penicillin G acylase from Achromobacter sp. for highly efficient biosynthesis of β-lactam antibiotics" @default.
- W4362721829 cites W1535989353 @default.
- W4362721829 cites W1970579816 @default.
- W4362721829 cites W1999421576 @default.
- W4362721829 cites W2012816104 @default.
- W4362721829 cites W2017898579 @default.
- W4362721829 cites W2024797379 @default.
- W4362721829 cites W2026043734 @default.
- W4362721829 cites W2034030618 @default.
- W4362721829 cites W2040262026 @default.
- W4362721829 cites W2048593042 @default.
- W4362721829 cites W2049708958 @default.
- W4362721829 cites W2058995008 @default.
- W4362721829 cites W2060493351 @default.
- W4362721829 cites W2077238505 @default.
- W4362721829 cites W2114367386 @default.
- W4362721829 cites W2143407053 @default.
- W4362721829 cites W2152321334 @default.
- W4362721829 cites W2158261322 @default.
- W4362721829 cites W2170711116 @default.
- W4362721829 cites W2242374221 @default.
- W4362721829 cites W2284465547 @default.
- W4362721829 cites W2295000077 @default.
- W4362721829 cites W24471328 @default.
- W4362721829 cites W2551493597 @default.
- W4362721829 cites W2742394249 @default.
- W4362721829 cites W2782093836 @default.
- W4362721829 cites W2810117176 @default.
- W4362721829 cites W2883202213 @default.
- W4362721829 cites W2888371121 @default.
- W4362721829 cites W2901818893 @default.
- W4362721829 cites W2946262315 @default.
- W4362721829 cites W2987860459 @default.
- W4362721829 cites W3006734129 @default.
- W4362721829 cites W3012272961 @default.
- W4362721829 cites W3087318638 @default.
- W4362721829 cites W3129180401 @default.
- W4362721829 cites W3148772518 @default.
- W4362721829 cites W3191391635 @default.
- W4362721829 cites W4285801616 @default.
- W4362721829 cites W4293382948 @default.
- W4362721829 cites W4297023518 @default.
- W4362721829 cites W4313334417 @default.
- W4362721829 doi "https://doi.org/10.1016/j.bioorg.2023.106533" @default.
- W4362721829 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37084587" @default.
- W4362721829 hasPublicationYear "2023" @default.
- W4362721829 type Work @default.
- W4362721829 citedByCount "0" @default.
- W4362721829 crossrefType "journal-article" @default.
- W4362721829 hasAuthorship W4362721829A5011549562 @default.
- W4362721829 hasAuthorship W4362721829A5019176281 @default.
- W4362721829 hasAuthorship W4362721829A5024015895 @default.
- W4362721829 hasAuthorship W4362721829A5024415495 @default.
- W4362721829 hasAuthorship W4362721829A5050302262 @default.
- W4362721829 hasAuthorship W4362721829A5070804547 @default.
- W4362721829 hasAuthorship W4362721829A5075363504 @default.
- W4362721829 hasAuthorship W4362721829A5078323046 @default.
- W4362721829 hasAuthorship W4362721829A5082156574 @default.
- W4362721829 hasConcept C161790260 @default.
- W4362721829 hasConcept C174802600 @default.
- W4362721829 hasConcept C185592680 @default.
- W4362721829 hasConcept C191503008 @default.
- W4362721829 hasConcept C21951064 @default.
- W4362721829 hasConcept C2776067932 @default.
- W4362721829 hasConcept C2776131168 @default.
- W4362721829 hasConcept C2776341680 @default.
- W4362721829 hasConcept C2777451570 @default.
- W4362721829 hasConcept C2779793070 @default.
- W4362721829 hasConcept C501593827 @default.
- W4362721829 hasConcept C55493867 @default.
- W4362721829 hasConcept C64778159 @default.
- W4362721829 hasConcept C65024703 @default.
- W4362721829 hasConcept C71240020 @default.
- W4362721829 hasConcept C94412978 @default.
- W4362721829 hasConceptScore W4362721829C161790260 @default.
- W4362721829 hasConceptScore W4362721829C174802600 @default.
- W4362721829 hasConceptScore W4362721829C185592680 @default.
- W4362721829 hasConceptScore W4362721829C191503008 @default.
- W4362721829 hasConceptScore W4362721829C21951064 @default.
- W4362721829 hasConceptScore W4362721829C2776067932 @default.
- W4362721829 hasConceptScore W4362721829C2776131168 @default.
- W4362721829 hasConceptScore W4362721829C2776341680 @default.
- W4362721829 hasConceptScore W4362721829C2777451570 @default.
- W4362721829 hasConceptScore W4362721829C2779793070 @default.
- W4362721829 hasConceptScore W4362721829C501593827 @default.